A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (<i>Danio rerio</i>) development

Beasley, Amy; Graham, Chris; Otter, Ryan R.; Elrod-Erickson, Matthew

doi:10.1002/etc.2428

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…The inclusion of molecular endpoints in toxicity testing methods that utilize early–life stage fishes has been advocated for previously . In the present study, we sought to determine whether zebrafish and fathead minnows from the FET and LGS tests experienced alterations in the expression of genes related to growth ( igf1 and igf2 ) and toxicity ( hsp70 , cyp1A , glulb ).…”

Section: Discussionmentioning

confidence: 99%

The fish embryo toxicity test as a replacement for the larval growth and survival test: A comparison of test sensitivity and identification of alternative endpoints in zebrafish and fathead minnows

Jeffries

Stultz

Smith

et al. 2015

Enviro Toxic and Chemistry

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The fish embryo toxicity (FET) test has been proposed as an alternative to the larval growth and survival (LGS) test. The objectives of the present study were to evaluate the sensitivity of the FET and LGS tests in fathead minnows (Pimephales promelas) and zebrafish (Danio rerio) and to determine if the inclusion of sublethal metrics as test endpoints could enhance test utility. In both species, LGS and FET tests were conducted using 2 simulated effluents. A comparison of median lethal concentrations determined via each test revealed significant differences between test types; however, it could not be determined which test was the least and/or most sensitive. At the conclusion of each test, developmental abnormalities and the expression of genes related to growth and toxicity were evaluated. Fathead minnows and zebrafish exposed to mock municipal wastewater-treatment plant effluent in a FET test experienced an increased incidence of pericardial edema and significant alterations in the expression of genes including insulin-like growth factors 1 and 2, heat shock protein 70, and cytochrome P4501A, suggesting that the inclusion of these endpoints could enhance test utility. The results not only show the utility of the fathead minnow FET test as a replacement for the LGS test but also provide evidence that inclusion of additional endpoints could improve the predictive power of the FET test.

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Section: Discussionmentioning

confidence: 99%

The fish embryo toxicity test as a replacement for the larval growth and survival test: A comparison of test sensitivity and identification of alternative endpoints in zebrafish and fathead minnows

Jeffries

Stultz

Smith

et al. 2015

Enviro Toxic and Chemistry

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“…By so doing, we were able to observe an increase in sensitivity of >30%. Other studies have reported relevant increases in sensitivity after addition of molecular (Hermsen et al ; Beasley et al ; Jeffries et al ), morphological (Lammer et al ; Brannen et al ; Di Paolo et al ), and behavioral endpoints to the FET (Di Paolo et al ), and thus this could be a fruitful solution to the problem of overcoming OECD 236 sensitivity issues. In addition to comparing LC50 values across fish tests for a specific effluent, we have shown ways to improve the sensitivity of the FET without extra costs or administrative burdens.…”

Section: Discussionmentioning

confidence: 95%

Is fish embryo test (FET) according to OECD 236 sensible enough for delivering quality data for effluent risk assessment?

Stelzer

Rosin

Bauer

et al. 2018

Enviro Toxic and Chemistry

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Over the past few years, the fish embryo test (FET) has become widely accepted as an animal-friendly protocol for ecotoxicological research. As Organisation for Economic Co-operation and Development (OECD) 236, the FET has been widely applied for simple mixture exposures under the Registration, Evaluation, Authorisation, and Restriction of Chemicals regulation of the European Union; and now its use is spreading worldwide as a supposedly reliable whole-effluent test (i.e., the testing of complex mixture exposures). However, comparative peer-reviewed data regarding the FET's efficiency for whole-effluent tests are virtually nonexistent. The primary objective of the present study was to make the first comparative test between the FET according to OECD 236 and other standard and slightly modified standard fish protocols used worldwide for whole-effluent tests. For that, we used an untreated hospital effluent considered to be highly toxic but disposed of in municipal sewerage. The base methods were OECD 203 (juvenile), US Environmental Protection Agency method 2000.0 (larvae), and OECD 236 (embryo). We also evaluated the addition of 3 virtually costless sublethal metrics (immobility, nonhatching, and pericardial edema) that could enhance the sensitivity of OECD 236. We observed acute toxicity in all 8 methodologies tested, with a clear escalation in sensitivity (larvae > juvenile ≥ embryo). Larvae were the most sensitive life stage for whole-effluent tests. The addition of sublethal metrics to OECD 236 enhanced its previous sensitivity in over 30%. Thus we conclude that OECD 236 acts below its potential and that the embryonic stage (as used in the FET) may not be the most sensitive life stage for whole-effluent tests. Environ Toxicol Chem 2018;37:2925-2932. © 2018 SETAC.

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Omics tools: New challenges in aquatic nanotoxicology?

2017

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A molecular method for assessing the effects of potential contaminants on the rate of zebrafish (Danio rerio) development

Cited by 4 publications

References 34 publications

The fish embryo toxicity test as a replacement for the larval growth and survival test: A comparison of test sensitivity and identification of alternative endpoints in zebrafish and fathead minnows

The fish embryo toxicity test as a replacement for the larval growth and survival test: A comparison of test sensitivity and identification of alternative endpoints in zebrafish and fathead minnows

Is fish embryo test (FET) according to OECD 236 sensible enough for delivering quality data for effluent risk assessment?

Omics tools: New challenges in aquatic nanotoxicology?

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