Considerations in the choice of a skin donor site for harvesting keratinocytes containing a high proportion of stem cells for culture in vitro

Lavoie, Arnelie; Fugère, Claudia; Fradette, Julie; Larouche, Danielle; Paquet, Claudie; Beauparlant, Annie; Gauvin, Robert; Tetu, Felix-Andre; Roy, Alphonse; Bouchard, Maurice; Genest, Hervé; Auger, François A.; Germain, Lucie

doi:10.1016/j.burns.2010.09.004

Cited by 10 publications

(4 citation statements)

References 30 publications

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“…A suitable alternative donor site for female paediatric burn patients remains an open question, such as the pre‐ or postauricular areas, as suggested by Lavoie et al . (). In vitro comparative studies of the proliferative and RHE reconstructive capacities of cells from different body sites could be helpful to select an optimal site for keratinocyte isolation before considering graft protocols in girls.…”

Section: Resultsmentioning

confidence: 97%

“…However, it appears difficult at the present time to extract the epithelial cell population from the hair follicles for clinical practice. These stem cells are deeply located in the basal layer of the outer root sheath (ORS), at the level of the hair follicle bulge (Lavoie et al , ). Given the difficulties of harvesting deep scalp in children, and given our in vitro results on foreskin keratinocyte regenerative capacities, we have proposed to graft foreskin keratinocytes (Mcheik et al , ).…”

Section: Resultsmentioning

confidence: 99%

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Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Mcheik

Barrault²,

Pedretti³

et al. 2013

J Tissue Eng Regen Med

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Severe burns in children are conventionally treated with split-thickness skin autografts or epidermal sheets. However, neither early complete healing nor quality of epithelialization is satisfactory. An alternative approach is to graft isolated keratinocytes. We evaluated paediatric foreskin and auricular skin as donor sources, autologous keratinocyte transplantation, and compared the graft efficiency to the in vitro capacities of isolated keratinocytes to divide and reconstitute epidermal tissue. Keratinocytes were isolated from surgical samples by enzymatic digestion. Living cell recovery, in vitro proliferation and epidermal reconstruction capacities were evaluated. Differentiation status was analysed, using qRT-PCR and immunolabelling. Eleven children were grafted with foreskin-derived (boys) or auricular (girls) keratinocyte suspensions dripped onto deep severe burns. The aesthetic and functional quality of epithelialization was monitored in a standardized way. Foreskin keratinocyte graft in male children provides for the re-epithelialization of partial deep severe burns and accelerates wound healing, thus allowing successful wound closure, and improves the quality of scars. In accordance, in vitro studies have revealed a high yield of living keratinocyte recovery from foreskin and their potential in terms of regeneration and differentiation. We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. In vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Mcheik

Barrault²,

Pedretti³

et al. 2013

J Tissue Eng Regen Med

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“…These results indicate that in addition to the choice of an adult anatomic site featuring a high number of stem cells in situ, the quality of the cultures greatly depends on the ability to extract stem cells from the skin biopsy. 82 …”

Section: Donor Sites: a Critical Choice For Successful Graftingmentioning

confidence: 99%

Epidermal Healing in Burns

Mcheik

Barrault²,

Levard

et al. 2014

Plastic and Reconstructive Surgery Global Open

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Background:Treatment of burned patients is a tricky clinical problem not only because of the extent of the physiologic abnormalities but also because of the limited area of normal skin available.Methods:Literature indexed in the National Center (PubMed) has been reviewed using combinations of key words (burns, children, skin graft, tissue engineering, and keratinocyte grafts). Articles investigating the association between burns and graft therapeutic modalities have been considered. Further literature has been obtained by analysis of references listed in reviewed articles.Results:Severe burns are conventionally treated with split-thickness skin autografts. However, there are usually not enough skin donor sites. For years, the question of how covering the wound surface became one of the major challenges in clinical research area and several procedures were proposed. The microskin graft is one of the oldest methods to cover extensive burns. This technique of skin expansion is efficient, but results remain inconsistent. An alternative is to graft cultured human epidermal keratinocytes. However, because of several complications and labor-intensive process of preparing grafts, the initial optimism for cultured epithelial autograft has gradually declined. In an effort to solve these drawbacks, isolated epithelial cells from selecting donor site were introduced in skin transplantation.Conclusions:Cell suspensions transplanted directly to the wound is an attractive process, removing the need for attachment to a membrane before transfer and avoiding one potential source of inefficiency. Choosing an optimal donor site containing cells with high proliferative capacity is essential for graft success in burns.

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“…In the self-assembly approach, two steps are affected by the serum used: ECM deposition to produce the stromal sheets as well as epithelium growth and differentiation. Studies have examined the effects of the origin of the mesenchymal [ 6 , 22 ] and epithelial cells [ 23 ] on the quality of tissues produced by the self-assembly technique but not the effects of the origin of the serum. The aim of this study was to determine if the serum origin had a significant impact on the quality of bladder and skin substitutes produced by the self-assembly technique.…”

Section: Introductionmentioning

confidence: 99%

Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach

et al. 2016

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Despite the emergence of serum-free media for cell culture, the use of serum to supplement the culture media is still essential in order to produce engineered urologic tissues using the self-assembly approach, not only for the stromal compartment but also for the uroepithelium. Effects of sera on thickness of these two compartments were measured and quality of the epithelial differentiation was evaluated. For bladder mucosa substitute reconstruction, the use of postnatal sera failed to produce an adequate uroepithelium whereas the fetal sera supplementation did. Postnatal sera also provided thinner stromal compartments than the one obtained using fetal sera, no matter if the fibroblasts from healthy or psoriatic donors were used to reconstruct human skin substitutes.

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Considerations in the choice of a skin donor site for harvesting keratinocytes containing a high proportion of stem cells for culture in vitro

Cited by 10 publications

References 30 publications

Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts

Epidermal Healing in Burns

Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach

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