Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach

Chabaud, Stéphane; Simard, Marie; Gendreau, Isabelle; Pouliot, Roxane; Bolduc, Stéphane

doi:10.1155/2016/3825645

Cited by 4 publications

(5 citation statements)

References 32 publications

(38 reference statements)

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“…Interestingly, the BF-derived tissues produced using NBCS were weaker ( Figure 6 A and Figure 7 A). Combined with the previously obtained results for the minimal differentiation of urothelial cells on the top of such a stroma [ 24 ], NBCS should be avoided to reconstruct urologic tissues. Such a result could be, in part, explained by the nature of BFs.…”

Section: Discussionmentioning

confidence: 92%

“…Adhesion, proliferation, and metabolism of nine fibroblast populations from three different organs were evaluated in 2D cell cultures. Fibroblasts from the skin, bladder, and vagina were chosen because the reconstruction of substitutes of these source organs using the self-assembly method that has been published [ 22 , 24 , 25 , 26 ]. The diversity of primary fibroblast populations in terms of organ type, anatomical sites, sex, and age constitutes one of the limitations of this article.…”

Section: Discussionmentioning

confidence: 99%

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Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts

et al. 2021

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Heat inactivation of bovine sera is routinely performed in cell culture laboratories. Nevertheless, it remains debatable whether it is still necessary due to the improvement of the production process of bovine sera. Do the benefits balance the loss of many proteins, such as hormones and growth factors, that are very useful for cell culture? This is even truer in the case of tissue engineering, the processes of which is often very demanding. This balance is examined here, from nine populations of fibroblasts originating from three different organs, by comparing the capacity of adhesion and proliferation of cells, their metabolism, and the capacity to produce the stroma; their histological appearance, thickness, and mechanical properties were also evaluated. Overall, serum inactivation does not appear to provide a significant benefit.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts

et al. 2021

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“…4 Our results showed that the source of serum influenced the production of ECM by cells in long-term cultures in a clearly species-dependent manner. Similarly, Chabaud et al 30 looked at the influence of eight different serum sources on the ECM production by human and rabbit dermal fibroblasts cultured for 4 weeks. Both species showed high serum-source dependency when final tissue thickness was evaluated.…”

Section: Discussionmentioning

confidence: 99%

Cell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells

et al. 2021

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We have created entirely biological tissue-engineered vascular grafts (TEVGs) using sheets of cell-assembled extracellular matrix (CAM) produced by human fibroblasts in vitro. A large animal TEVG would allow long-term pre-clinical studies in a clinically relevant setting (graft size and allogeneic setting). Therefore, canine, porcine, ovine, and human skin fibroblasts were compared for their ability to form CAM sheets. Serum sourcing greatly influenced CAM production in a species-dependent manner. Ovine cells produced the most homogenous and strongest animal CAM sheets but remained ≈3-fold weaker than human sheets despite variations of serum, ascorbate, insulin, or growth factor supplementations. Key differences in cell growth dynamics, tissue development, and tissue architecture and composition were observed between human and ovine. This study demonstrates critical species-to-species differences in fibroblast behavior and how they pose a challenge when attempting to substitute animal cells for human cells during the development of tissue-engineered constructs that require long-term cultures.

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“…In 2011, our group reported that a self-assembled human skin model, composed of healthy fibroblasts and keratinocytes, could produce a well-differentiated epidermis independently of the presence or absence of serum during the culture process [29] . These results suggest that using calf serum at an early state during the production of the substitutes is suitable to allow cell growth while using serum-deprived medium at an advanced state is acceptable to reduce unforeseen interactions and to avoid individual differences between serum lots [30] .…”

Section: Introductionmentioning

confidence: 90%

A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions

Duque-Fernandez

Gauthier

Simard

et al. 2016

Biochemistry and Biophysics Reports

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BackgroundInadequate representation of the human tissue environment during a preclinical screen can result in inaccurate predictions of compound effects. Consequently, pharmaceutical investigators are searching for preclinical models that closely resemble original tissue for predicting clinical outcomes.MethodsThe current research aims to compare the impact of using serum-free medium instead of complete culture medium during the last step of psoriatic skin substitute reconstruction. Skin substitutes were produced according to the self-assembly approach.ResultsSerum-free conditions have no negative impact on the reconstruction of healthy or psoriatic skin substitutes presented in this study regarding their macroscopic or histological appearances. ATR-FTIR results showed no significant differences in the CH2 bands between psoriatic substitutes cultured with or without serum, thus suggesting that serum deprivation did not have a negative impact on the lipid organization of their stratum corneum. Serum deprivation could even lead to a better organization of healthy skin substitute lipids. Percutaneous analyses demonstrated that psoriatic substitutes cultured in serum-free conditions showed a higher permeability to hydrocortisone compared to controls, while no significant differences in benzoic acid and caffeine penetration profiles were observed.ConclusionsResults obtained with this 3D-psoriatic skin substitute demonstrate the potential and versatility of the model. It could offer good prediction of drug related toxicities at preclinical stages performed in order to avoid unexpected and costly findings in the clinic.General significanceTogether, these findings offer a new approach for one of the most important challenges of the 21st century, namely, prediction of drug toxicity.

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Origin of Serum Affects Quality of Engineered Tissues Produced by the Self-Assembly Approach

Cited by 4 publications

References 32 publications

Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts

Heat-Inactivation of Fetal and Newborn Sera Did Not Impair the Expansion and Scaffold Engineering Potentials of Fibroblasts

Cell-assembled extracellular matrix (CAM) sheet production: Translation from using human to large animal cells

A 3D-psoriatic skin model for dermatological testing: The impact of culture conditions

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