Conservation of microsatellite regions across legume genera enhances marker repertoire and genetic diversity study in Phaseolus genotypes

Abstract

To study the conservation of microsatellite regions, a set of 137 microsatellite markers developed from Phaseolus, Cajanus, Lens and Cicer genera of Leguminosae family were tested for their transferability across 16 genotypes of Phaseolus belonging to diverse collections from South America and Asia. Considerable transferability was observed with markers derived from Cajanus (60%), Lens (46%) and Cicer (28%). Of the total 122 loci were amplified, 82 cross-species polymorphic amplicons were obtained. Maximum number of alleles per marker was six (Cicer markers). Polymorphism information content values ranged from 0.12 to 0.96 with Cajanus, 0.13 to 0.74 with Lens and 0.30 to 0.93 with Cicer markers. Unweighted pair group method employing arithmetic averages cluster analysis of Phaseolus genotypes showed clear demarcation between the commercial cultivars falling in separate cluster with respect to their seed size and maturity. Transferability of genomic SSRs was different from that of expressed sequence tag-derived genic microsatellite markers.

Potential use of SSR and ISSR markers in estimation of DNA polymorphism within genus Vicia

“…nigra and V. grandiflora. Average transferability of SSR markers was 56.25% which was in concordance with the previously published results on legumes (Reddy et al 2010, Datta et al 2012, Gupta et al 2013). Figure 2), with exception of Vicia narbonensis and Vicia faba, which had the same banding pattern with primer (GA)8T (Table 4).…”

Detection and characterization of polymorphic simple sequence repeats markers for the analysis of genetic diversity in Indian mungbean [Vigna radiata (L.) Wilczek]

“…Thus there is a need to screen and identify polymorphic SSR markers in greater numbers to facilitate analyses of genetic diversity in mungbean. The process may include transferring the SSR markers from other closely related legumes such as moth bean, adzuki bean, cowpea and rice bean as this will also facilitate correlated studies in across related crops (Datta et al 2013;Jingade et al 2014;Shivakumar et al 2017).…”

Cross Legume Species/Genera Transferability of SSR Markers and their Utility in Assessing Polymorphism among Advanced Breeding Lines in Dolichos Bean (Lablab purpureus L.)

“…(Gupta et al, 2012) have reported 92 per cent transferability of greengram SSR markers, 91 per cent of adjuki bean SSR markers and 86 per cent of cowpea SSR markers to black gram. (Datta et al, 2013) reported highest transferability of SSR markers from common bean followed by lentil, field pea and chickpea to pigeonpea. (Agbagwa et al, 2015) reported successful transferability of SSR markers from Vigna savi to pigeonpea.…”

“…The estimates of PIC of transferable SSR markers from Vigna savi to cultivated and wild relatives of Cajanus adams ranged from 0.24 to 0.69 with an average of 0.47 (Agbagwa et al, 2015). Datta et al, (2013) reported a fairly high estimates of PIC (ranging from 0.5 to 0.6) of cross transferable SSR markers from common bean, lentil, field pea and chickpea to pigeonpea. The reported significant differences in quantitative traits means of 14 ABL were amply reflected at transferable SSR marker allelic diversity in the present study.…”