Conservation of a 31-bp bovine subrepeat in centromeric satellite DNA monomers ofCervus elaphus and other cervid species

Abstract

A centromeric satellite DNA clone was isolated from the genome of the European red deer (Cervus elaphus hippelaphus) and designated Ce-Pst1. This clone was localized to the centromeric region of all red deer chromosomes with the exception of a single pair of metacentric autosomes and the Y chromosome. DNA sequence analysis of the 806-bp Ce-Pst1 clone showed 73.0-78.9% sequence homology to four previously isolated cervid centromeric satellite DNA clones, suggesting that the Ce-Pst1 clone is yet another member of the major cervid centromeric satellite DNA family. Using a DNA sequence comparison system, internal 31-bp tandem subrepeats were found in the Ce-Pst1 clone as well as in the other previously reported cervid centromeric satellite DNA monomer sequences. A 31-bp consensus sequence was constructed for each cervid monomer clone and shown to be highly homologous to the 31-bp subrepeat consensus sequence found in bovine 1.715 centromeric satellite DNA. The identification of internal subrepeats in the satellite monomers studied could suggest that amplification of an ancestral 31-bp DNA sequence may have contributed to the genesis of major cervid centromeric satellite DNA. The homology between the 31-bp subrepeats found in cervid and bovid centromeric satellite DNAs substantiates the theory that amplification of this 31-bp DNA sequence may have occurred before the evolutionary separation of these two families 20-25 million years ago.

Cloning, Characterization, and FISH Mapping of Four Satellite DNAs from Black Muntjac (<I>Muntiacus crinifrons</I>)and Fea's Muntjac (<I>M. feae</I>)

“…Some studies suggested that the repetitive DNA families at or near the centromeric and telomeric regions might facilitate illegitimate recombination between non-homologous chromosomes of muntjacs (Brinkley et al, 1984;Bogenberger et al, 1985Bogenberger et al, , 1987Benedum et al, 1986;Lin et al, 1991Lin et al, , 2004Lee et al, 1994Lee et al, , 1997Scherthan, 1995;Lee & Lin, 1996;Yang et al, 1997b;Li et al, 2000aLi et al, , b, 2002Hartmann & Scherthan, 2004). At present, four satellite DNA families are found in Muntiacus: satellite DNA families I, II, IV and V (Bogenberger et al, 1985;Lin et al, 1991Lin et al, , 2004Li et al, 2000bLi et al, , 2005.…”

Centromere Evolution: Digging into Mammalian Primary Constriction

“…Quite common features of satellite DNA are: i) the presence of internal direct and inverted subrepeats (Bogenberger et al, 1985;Lee, C. & Lin, 1996;Zhang & Horz, 1984); ii) the presence of the same satellite DNA families at the centromeres of autosomes and the X chromosome, but not at the Y chromosome centromere, like in mouse, sheep, and bat; iii) a greater divergence of the Y centromere sequence in comparison to the other centromeres, like in mouse and primates. Sheep, swine, and horse have a different satellite content in acrocentric and metacentric chromosomes.…”

“…The presence of this 31 bp motif across the entire length of the satellite I repeat suggests that its present structure could have arisen from a tandemly amplification of an ancestral ~31 bp unit. The 31-mer motif sequence has been found also in other bovine satellites, like satellite III (Plucienniczak et al, 1982;Taparowsky & Gerbi, 1982), and in the deer, muntjac, and pronghorn centromeric satellites (Bogenberger et al, 1985;Denome et al, 1994;Lee, C. & Lin, 1996), arguing that the amplification of the ~31 bp unit may have occurred in their common ancestral.…”

Pericentric satellite DNA sequences in Pipistrellus pipistrellus (Vespertilionidae; Chiroptera)

“…A very common feature of satellite DNAs is the presence of internal direct and inverted subrepeats (Zhang and Horz, 1984;Bogenberger et al, 1985;Lee and Lin, 1996). Nevertheless, no internal subrepeats were found in this satellite of P. pipistrellus.…”

“…Hence, the location of this repetitive DNA coincides with the location of the pericentromeric constitutive heterochromatin previously described. The presence of satellite DNA in the centromeres of autosomes and X chromosomes and its absence from the Y chromosome centromere is a common feature of satellite DNAs from other mammal species (Lee and Lin, 1996;Kunze et al, 1999;Fernández et al, 2001).…”