Pericentric satellite DNA sequences in Pipistrellus pipistrellus (Vespertilionidae; Chiroptera)

Barragán, María J. López; Martínez, Sérgio; Marchal, Juan A.; Fernández, Rosa; Bullejos, Mónica; Guardia, R. Díaz de la; Sánchez, Antonio

doi:10.1038/sj.hdy.6800303

Cited by 5 publications

(8 citation statements)

References 41 publications

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“…The FISH procedure followed was essentially that of Barragán et al (2003). The probe (clone R.hip-14) was PCR-labelled with biotin-16-dUTP (Roche) following the protocol described by Lo et al (1990).…”

Section: Chromosome Preparations and Fluorescence In Situ Hybridizatimentioning

confidence: 99%

The karyotype and 5S rRNA genes from Spanish individuals of the bat species Rhinolophus hipposideros (Rhinolophidae; Chiroptera)

et al. 2007

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The karyotype of individuals of the species Rhinolophus hipposideros from Spain present a chromosome number of 2n = 54 (NFa = 62). The described karyotype for these specimens is very similar to another previously described in individual from Bulgaria. However, the presence of one additional pair of autosomal acrocentric chromosomes in the Bulgarian karyotype and the differences in X chromosome morphology indicated that we have described a new karyotype variant in this species. In addition, we have analyzed several clones of 1.4 and 1 kb of a PstI repeated DNA sequence from the genome of R. hipposideros. The repeated sequence included a region with high identity with the 5S rDNA genes and flanking regions, with no homology with GenBank sequences. Search for polymerase III regulatory elements demonstrated the presence of type I promoter elements (A-box, Intermediate Element and C-box) in the 5S rDNA region. In addition, upstream regulatory elements, as a D-box and Sp1 binding sequences, were present in flanking regions. All data indicated that the cloned repeated sequences are the functional rDNA genes from this species. Finally, FISH demonstrated the presence of rDNA in nine chromosome pairs, which is surprising as most mammals have only one carrier chromosome pair.

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Section: Chromosome Preparations and Fluorescence In Situ Hybridizatimentioning

confidence: 99%

The karyotype and 5S rRNA genes from Spanish individuals of the bat species Rhinolophus hipposideros (Rhinolophidae; Chiroptera)

et al. 2007

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“…Although not common, other repeated DNA sequences have also been described in Y chromosome pericentromeric heterochromatin of Microtus species, and in this sense, Msat-160 is present in that Y chromosome region of M. miurus and M. guentheri Modi et al, 2003). However, the distribution on the centromeres of autosomes and X chromosomes but not on the Y chromosome centromere is a common feature of satellite DNAs in most mammalian species (Lee and Lin, 1996;Kunze et al, 1999;Fernández et al, 2001;Barragán et al, 2003).…”

Section: Mth-alu900-con Actggtcaaaccggttta---------------------------mentioning

confidence: 99%

A new pericentromeric repeated DNA sequence in <i>Microtus thomasi</i>

Acosta

Marchal

Mitsainas

et al. 2009

Cytogenet Genome Res

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Several karyotypic forms have been previously described in populations of the vole species Microtus thomasi from Greece. In particular, the karyomorphs Microtus thomasi ‘thomasi’ and ‘atticus’ differ in X chromosome morphology, being acrocentric and subtelocentric, respectively. Furthermore, remarkable heterochromatin content variability has been described in sex chromosomes of both karyomorphs. Genomic DNA digestion with AluI allowed us to clone an 884 bp long repeated DNA sequence (Mth-Alu900) from the karyomorph M. thomasi ‘atticus’. This repeated DNA is AT rich and seems to be organized mainly as a dimer of the 884-bp unit, which presents three simple repeats (CAAAT, CAGAT and CAGAC) that constitute 80% of the total unit length. This repeated DNA is exclusive to M. thomasi, since it is absent from the genome of other studied Arvicolinae species. The chromosomal location of Mth-Alu900 was analyzed on M. thomasi ‘thomasi’ and M. thomasi ‘atticus’ karyomorphs, with different sex chromosome constitution. It was mainly located on the pericentromeric heterochromatin of most autosomes and X chromosomes on both karyomorphs. Results are also discussed in relation to karyotypic and sex chromosome variations in M. thomasi. To our knowledge, Mth-Alu900 constitutes a new – the third discovered so far – pericentromeric repeated DNA sequence described in Microtus species.

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“…Furthermore, both clones showed a lower percentage of identity versus the consensus sequence (87%), whereas for the remaining clones this percentage was always over 97%. The presence of specific mutations in the same position as several repeats have been described in certain satellite DNAs in several bat species [12][13][14]. Many authors consider these characteristics the result of concerted evolution, which can act in the several ways in the satellite DNA evolution.…”

Section: Sequence Organization and Intraspecific Variabilitymentioning

confidence: 99%

“…In support of PettigrewÕs ''flying DNA'' hypothesis, Van Den Bussche et al [11] have documented that the events that have achieved or maintained the small genome size in the bat Macrotus waterhousii have resulted in a lower copy number of interspersed and tandemly repetitive elements. On the other hand, the existence of one or more families of repetitive DNA has been described in several bat species [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the first satellite DNA with CENP‐B and CDEIII motifs in the bat Pipistrellus kuhli

Fantaccione¹,

Pontecorvo²,

Zampella³

2005

FEBS Letters

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The centromere is an essential structure in the chromosomes of all eukariotes and is central to the mechanism that ensures proper segregation during mitosis and meiosis. The comparison of DNA sequence motifs, organization and kinetocore components from yeast to man is beginning to indicate that, although centromeres are highly variable DNA elements, a conserved pattern of sequence arrangement and function is emerging. We have identified and characterized the first satellite DNA (P.k.SAT) from microbat species Pipistrellus kuhli. The presence of mammalian CENP-B box and yeast CDEIII box could indicate the participation of P.k.SAT in centromere organization.

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Pericentric satellite DNA sequences in Pipistrellus pipistrellus (Vespertilionidae; Chiroptera)

Cited by 5 publications

References 41 publications

The karyotype and 5S rRNA genes from Spanish individuals of the bat species Rhinolophus hipposideros (Rhinolophidae; Chiroptera)

The karyotype and 5S rRNA genes from Spanish individuals of the bat species Rhinolophus hipposideros (Rhinolophidae; Chiroptera)

A new pericentromeric repeated DNA sequence in <i>Microtus thomasi</i>

Molecular characterization of the first satellite DNA with CENP‐B and CDEIII motifs in the bat Pipistrellus kuhli

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