Conservation genetics of xerothermic beetles in Europe: the case of Centricnemus leucogrammus

Kajtoch, Łukasz

doi:10.1007/s10841-011-9377-8

Cited by 17 publications

(30 citation statements)

References 51 publications

(49 reference statements)

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“…The diversity of the nuclear EF1‐α gene is low in P. inustus , but this gene has only one single variant in E. ovulum . The interpretation of these results needs a broader context, eg through comparison with some sexual species such as the weevil Centricnemus leucogrammus , which also belongs to Entiminae and inhabits steppes (Kajtoch & Lachowska‐Cierlik, ; Kajtoch, ). This species has much higher genetic diversity in both mitochondrial and nuclear DNA (nucDNA), which supports the statement that parthenogenetic weevils have reduced genetic diversity compared with bisexual species.…”

Section: Discussionmentioning

confidence: 99%

Selective sweep of Wolbachia and parthenogenetic host genomes – the example of the weevil Eusomus ovulum

Mazur

Holecová

Lachowska‐Cierlik

et al. 2016

Insect Molecular Biology

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Most parthenogenetic weevil species are postulated to have originated via hybridization, but Wolbachia has also been speculated to play a role via the induction of parthenogenesis. Here, we examine the molecular diversity of Wolbachia and parthenogenetic host genomes. The host species studied here, Eusomus ovulum, is known to be exclusively parthenogenetic and triploid. The E. ovulum populations that we examined had a low genetic diversity of mitochondrial (cytochrome oxidase I gene) and nuclear markers (internal transcribed spacer 2 and elongation factor 1-α gene), and they all were infected by only single bacteria strains (genotyped for five genes according to the multilocus sequence typing system). We found significant signs of linkage disequilibrium and a lack of recombination amongst all of the examined genomes (bacteria and host), which strongly indicates a selective sweep. The lack of heterozygosity in host nuclear genes, missing bisexual populations and selective sweep between the parthenogenetic host and bacteria genomes suggest that parthenogenesis in this species could have originated as a result of infection rather than hybridization. However, the finding that highly similar Wolbachia strains are also present in other parthenogenetic weevils from the same habitat suggests the opposite scenario: bacteria may have infected the already parthenogenetic lineage and taken advantage of the host's unisexual reproduction.

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Section: Discussionmentioning

confidence: 99%

Selective sweep of Wolbachia and parthenogenetic host genomes – the example of the weevil Eusomus ovulum

Mazur

Holecová

Lachowska‐Cierlik

et al. 2016

Insect Molecular Biology

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“…The concentration of the reactives used for the amplifications and the cycling profiles for the polymerase chain reaction (PCR) of COII, ITS2 and EF1‐α markers were as in Kajtoch et al . (2009) and Kajtoch (2011). Wolbachia genes were screened as in Lachowska et al .…”

Section: Methodsmentioning

confidence: 99%

Genetic distinctness of parthenogenetic forms of European Polydrusus weevils of the subgenus Scythodrusus

2011

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Among eight species of Polydrusus weevils which belong to subgenus Scythodrusus, at least two possess parthenogenetic forms: P. (S.) inustus and P. (S.) pilifer. Both of these species consist of dioecious populations in the Caspian area and of parthenogenetic populations in Eastern Europe (P. (S.) inustus), the Caucasus region (both species) and Middle Asia (P. (S.) pilifer). The origin of parthenogenesis in this subgenus is unresolved; however some data suggest that the parthenogenetic forms are of hybrid ancestry. The genetic distinctness of parthenogenetic Scythodrusus was assessed on the basis of COII, ITS2, EF1‐α and Wolbachiawsp, 16S ribosomal DNA, ftsZ and hcpA sequence comparisons. Both taxa turned out to be monophyletic for all markers, which is an evidence against hybridization of their dioecious ancestors. On the other hand, a high frequency of heterozygous P. (S.) inustus females suggests an origin resulting from hybridization between genetically distinct dioecious representatives of this species. Very similar strains of Wolbachia supergroup A were found in both species, indicating that they have been either inherited from a common ancestor or were transmitted between parthenogenetic Scythodrusus weevils and probably spread randomly across their ranges.

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“…Additionally, EF1-α and Internal Transcribed Spacer 1 (ITS1) nuclear markers, also frequently used in phylogenetic studies of insects, were amplified using primer pairs EFs149 and EFα1R [30,31] and ITS1 and ITS2 [32]. The concentration of the reagents used for the amplification of COI, ITS1 and EF1-α markers and the cycling profile for PCR have been described previously [33,34]. After purification (NucleoSpin Extract II, Macherey-Nagel), the PCR fragments were sequenced using a BigDye Terminator v.3.1.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Molecular barcoding for central-eastern European Crioceris leaf-beetles (Coleoptera: Chrysomelidae)

et al. 2011

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Among Crioceris leaf-beetles, the two most widespread species (Crioceris asparagi and C. duodecimpunctata) are serious invasive plant pests, while another two (C. quatuordecimpunctata and C. quinquepunctata) are rare species restricted to steppe-like habitats in Eurasia. The aim of the research was to check the genetic distinctiveness of these four species and develop barcodes for their molecular identification using the mitochondrial Cytochrome Oxidase I (COI) gene and two nuclear markers: Elongation Factor 1-α (EF1-α) and Internal Transcribed Spacer 1 (ITS1). The identification of each species was possible and reliable with the use of COI and ITS1 markers. EF1-α was omitted in analyses due to its high level of heterozygosity (presence of multiple PCR products). C. duodecimpunctata and C. quatuordecimpunctata were shown to be sister taxa, but the similar genetic distances between all of the species indicate that these species originated almost simultaneously from a common ancestor. Identification of two separate clades in populations of C. quatuordecimpunctata suggested that the clades are isolated and can be considered as separate conservation units.

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Conservation genetics of xerothermic beetles in Europe: the case of Centricnemus leucogrammus

Cited by 17 publications

References 51 publications

Selective sweep of Wolbachia and parthenogenetic host genomes – the example of the weevil Eusomus ovulum

Selective sweep of Wolbachia and parthenogenetic host genomes – the example of the weevil Eusomus ovulum

Genetic distinctness of parthenogenetic forms of European Polydrusus weevils of the subgenus Scythodrusus

Molecular barcoding for central-eastern European Crioceris leaf-beetles (Coleoptera: Chrysomelidae)

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