Consequence of Lepidium Sativum Seeds on Flat of Biochemical Parameters in Albino Male Rats Treated with Acetaminophen

Abstract

Experiment is to assess efficiency of alcoholic extract of Lepidium sativum seeds in improving erythropoietin levels and some other biological parameters against acetaminophen-induced hepatotoxicity and nephrotoxicity. The study was worked in Labs / Unive. Kufa for the period from 1/10/2021 to 1/12/2021, with a follow-up of (42) three-month-old male albino rats, their weights ranged between 215-242 g to assess the protective role of the alcoholic extract of Lepidium sativum seeds in inhibiting acetaminophen-induced hepatotoxicity and nephrotoxicity, the rats were divided into six groups with seven animals for each group. The first group, which is the control group, and they were dosed with tap water once a day, the second group, that were orally dosed with acetaminophen 1000mg\kg once, and the third group was treated with the alcoholic extract of Lepidium sativum seeds at a concentration of 250 mg/kg and an hour later was given acetaminophen 1000 mg/kg orally, while the fourth group was treated with alcoholic extract of Lepidium sativum seeds at a concentration of 500 mg/kg and an hour later they were given acetaminophen 1000 mg/kg orally, and the fifth group was treated with alcoholic extract of Lepidium sativum seeds at a concentration of 250 mg/kg orally only, and with regard to the sixth group, it was treated orally with the alcoholic extract of Lepidium sativum seeds at a concentration of 500 mg/kg only, and the treatment continued for a period of 60 days, once a day, in the morning (at half past eight). The study included evaluation of some biochemical parameters such as levels of erythropoietin hormone, iron ion, total iron binding capacity, total bilirubin, creatinine, urea, reduced and oxidized glutathione, malondialdehyde and lipid peroxide, decreasing of erythropoietin, iron ion, total iron binding capacity, reduced and oxidized glutathione, and important increase (P<0.05) in the levels of total bilirubin, creatinine, urea, malondialdehyde and lipid peroxidation in the group that was treated with the drug compared to the control group. As for the group that was given the alcoholic extract of Lepidium sativum seeds at a concentration of 250 mg/kg + acetaminophen, it did not show a protective role for this concentration against the toxic effects of the drug, as it showed important differences (P<0.05) in all parameters included in the current study compared with the control group. Contrary to above, the group that was given the alcoholic extract of Lepidium sativum seeds at a concentration of 500 mg/kg + acetaminophen did not reveal important alterations (P 0.05) in the study criteria compared with the control group. Concern to the group of rats treated with alcoholic extract of Lepidium sativum seeds concentration 250 mg/kg only, it did not give important differences in the study parameters compared with the control group, except for the level of reduced glutathione, which showed important increase (P<0.05). Finally, the group that orally dosed the alcoholic extract of Lepidium sativum seeds at a concentration of 500 mg/kg only, recorded important elevation (P<0.05) in the levels of erythropoietin hormone, iron ion, total iron binding capacity, reduced and oxidized glutathione, and in contrast important reduction (P<0.05) in the levels of total bilirubin, creatinine, urea, malondialdehyde and lipid peroxide in comparison with the control group.