“…Nevertheless, no rationale for the selection of the female dog was immediately apparent [2][3][4][5][6][7][8][9][10][11], However, Sm ith [12] in his textbook Principals o f Renal Physiology stated that the reasons female dogs are used are (1) they are 'fairly resistant to bladder infection' and (2) 'rigid sterile technique' is unnecessary. However, the use of female dogs has serious disadvantages; dogs have to be restrained and trained [1,3,4,8,[11][12][13]; in addition, an episiotomy must be performed [9,10]. Due to the nature of our experiment, valuable time was lost and the condition of the dogs deteriorated before renal clearances could be instituted.…”