Connective tissue composition and collagen synthesis in diseased and normal gingiva of adult dogs with spontaneous periodontitis

Page, Roy C.; Narayanan, A. Sampath; Schroeder, Hubert E.

doi:10.1016/0003-9969(80)90126-0

Cited by 14 publications

(4 citation statements)

References 21 publications

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“…In fact, the concentrations of prostaglandin E2 and cAMP increase in the gingival tissues with increasing severity of the disease (33,34,35). In addition, collagen-poor, cell-rich gingival connective tissue incorporates more [G-'H]-proline in vitro than does healthy tissue and a larger portion of the protein-bound activity is found in the culture medium (36) suggesting an increased synthesis of collagen in the diseased tissue. Since there is no accumulation of collagen, there is reason to believe that the reported increase in synthesis is counterbalanced by an enhanced intracellular degradation of nascent collagen.…”

Section: Discussionmentioning

confidence: 99%

Hydroxyproline determination in serum and gingival crevicular fluid

Svanberg

1987

J of Periodontal Research

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A high-performance hquid chromatographic assay for total hydroxyproline in serum and gingival crevicular fiuid has been developed. The Clq component of the first complement factor was precipitated out of the samples with a 0.02 M sodium acetate solution. Ahquots of the supernatants were dried, vapor hydrolyzed with 6 M hydrochloric acid at 105°C for 24 hours and derivatized with phenylisothiocyanate. The derivatives were chromatographed using a multilinear solvent gradient with detection at 254 nm. The regression of peak areas on the concentrations of hydroxyproline was linear with a correlation coefficient of 0,99 within the range of assayed quantities (2.5 to 25 ng). Analytical recovery was 90.5 ±2.54% (X + SD) and the determination level was 2.5 ng (19.1 pmol). The precision of the assay as determined by the coefficient of variation for five consecutive runs of six concentrations of hydroxyproline in serum hydrolysate ("within-run" precision) and five series run on different days ("between-run" precision) was 1.8+1.28% and 4.2 + 2.59% (X + SD), respectively. Hydroxyproline concentrations in GCF from single sites during developing experimental gingivitis in the beagle dog showed an irregular pattern of low and high concentrations ranging from 5.2 to 17.4 ng/fA.

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Section: Discussionmentioning

confidence: 99%

Hydroxyproline determination in serum and gingival crevicular fluid

Svanberg

1987

J of Periodontal Research

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“…The increased synthesis of enzymes related to the degradation of collagen provokes the initiation and advancing of periodontal disease accompanied by a 70% loss of the collagen. It is established that disintegration of collagen can be associated with the release of hydroxyproline (Hyp), which represents approximately 10% of this protein (43)(44)(45)(46)(47)(48). About 60-70 % of the collagen is destroyed in the region affected by gingival inflammation.…”

Section: Salivary Amino Acids and Periodontal Health Statusmentioning

confidence: 99%

“…The quantity of collagen-derived Hyp in gingival crevicular fluid (GCF) corresponds to the intensity of collagen loss and the activity of the pathological process in periodontal diseases. Researchers focus on the potentials of Hyp in GCF as an indicative parameter for loss of collagen in progressive periodontal disease (43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57).…”

Section: Salivary Amino Acids and Periodontal Health Statusmentioning

confidence: 99%

The role and significance of some salivary amino acids in periodontal diseases, including plaque-induced gingivitis—a literature review

Angelova,

Salim,

Kiselova-Kaneva

et al. 2021

Scripta Scientifica Medicinae Dentalis

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Nowadays the problem of plaque-induced gingivitis, including in the different periods of childhood, is marked by a considerable long-term sociomedical impact on individual, group, population and community levels globally. The purpose of this investigation is to study, analyze and summarize various scientific sources on the role and significance of some salivary amino acids in the presence of periodontal diseases, including plaque-induced gingivitis. This manuscript is based on data obtained from research articles, randomized trials, and systemic reviews published in PubMed and Google Scholar. Different salivary markers are characterized by potential for earlier diagnosis of the progression of oral diseases. We can conclude that some salivary amino acids play a considerable role in the periodontal status, including in plaque-induced gingivitis.

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“…Tissue changes due to plaque irritation could be the result of an unbalaneed synthesis of eonnective tissue components. In experimental periodontitis including its spontaneous form in dogs, the numher of fibrobiasts per volume of tissue is not significantly altered although their proportion from total eell population is decreased due to the high number of inflammatory cells (Schroeder & Lindhe 1980, Page, Narayanan & Schroeder 1980. The purpose of this study was to elucidate the meehanisms by which the induced synthesis of hyaluronic acid is regulated in gingival fibroblasts exposed to dental plaque extract.…”

Section: Introductionmentioning

confidence: 98%

Metabolic change in cultured gingival fibroblasts exposed to bacterial extracts

Larjava

1984

J of Periodontal Research

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Hyaluronic acid synthesis was studied in human gingival fibroblast cultures exposed to bacterial extracts from dental plaque, Bacillus cereus, and Escherichia coli (lipopolysaccharide, LPS). Dental plaque extract was the best stimulant of hyaluronic acid synthesis. B. cereus extract was almost as effective, but the effect of LPS was insubstantial. This indicates that gram‐positive microorganisms of dental plaque, as well as gram‐negative bacteria, may be involved in the stimulation of hyaluronic acid synthesis in gingival fibroblasts. The effect of indomethacin, cortisol, and cycloheximide on hyaluronic acid synthesis were studied in control and plaque extract treated cultures. Indomethacin had no effect whereas cortisol inhibited the synthesis both in control and treated cultures. Cycloheximide prevented the stimulation of hyaluronic acid synthesis in treated cultures. This suggests that protein synthesis is required for the stimulation of hyaluronic acid synthesis in the presence of plaque extract. The altered metabolism of connective tissue cells due to dental plaque may be important in the development of periodontal disease.

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Connective tissue composition and collagen synthesis in diseased and normal gingiva of adult dogs with spontaneous periodontitis

Cited by 14 publications

References 21 publications

Hydroxyproline determination in serum and gingival crevicular fluid

Hydroxyproline determination in serum and gingival crevicular fluid

The role and significance of some salivary amino acids in periodontal diseases, including plaque-induced gingivitis—a literature review

Metabolic change in cultured gingival fibroblasts exposed to bacterial extracts

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