1987
DOI: 10.1111/j.1600-0765.1987.tb01552.x
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Hydroxyproline determination in serum and gingival crevicular fluid

Abstract: A high-performance hquid chromatographic assay for total hydroxyproline in serum and gingival crevicular fiuid has been developed. The Clq component of the first complement factor was precipitated out of the samples with a 0.02 M sodium acetate solution. Ahquots of the supernatants were dried, vapor hydrolyzed with 6 M hydrochloric acid at 105°C for 24 hours and derivatized with phenylisothiocyanate. The derivatives were chromatographed using a multilinear solvent gradient with detection at 254 nm. The regress… Show more

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Cited by 21 publications
(6 citation statements)
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“…These constituents include physiologically active substances and enzymes such as prostaglandins,[1819] collagenase,[20] hydroxyproline,[21] β-glucuronidase,[22] lactate dehydrogenase,[22] glycosaminoglycans,[23] aspartate aminotransferase,[24] and alkaline phosphatase (ALP). [171925]…”
Section: Discussionmentioning
confidence: 99%
“…These constituents include physiologically active substances and enzymes such as prostaglandins,[1819] collagenase,[20] hydroxyproline,[21] β-glucuronidase,[22] lactate dehydrogenase,[22] glycosaminoglycans,[23] aspartate aminotransferase,[24] and alkaline phosphatase (ALP). [171925]…”
Section: Discussionmentioning
confidence: 99%
“…Alternative markers of collagen degradation in GCF such as hydroxyproline and the carboxy‐terminal telopeptide of type I collagen (ICTP) have also been evaluated (20–22). The pyridinoline crosslinked ICTP in particular has been suggested to offer a promising option to detect active periodontal destruction (23–27).…”
Section: Discussionmentioning
confidence: 99%
“…Gingival crevicular fluid (GCF) carries most of the breakdown products out of tissues, and these subsequently appear in the gingival sulcus [19]. There should be a small amount of GCF even in healthy gingiva, according to the osmotic gradient theory of ALFANO [20,21].…”
Section: Introductionmentioning
confidence: 99%