Connections between the Cell Cycle and the DNA Damage Response in Plants

Gentric, Naomie; Genschik, Pascal; Noir, Sandra

doi:10.3390/ijms22179558

Cited by 16 publications

(10 citation statements)

References 143 publications

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“…Here, CDKB2;2 was selected as a marker for cell division in apple fruit and cell culture. CDKA and CDKB are core cell cycle genes [17,32]. Two types of CDKBs i.e.…”

Section: Phylogenetic Analysis Of Cdk Genesmentioning

confidence: 99%

“…CDKB1 and CDKB2 are plant-specific. CDKB1 is expressed from the late S to M phases of the cell cycle, whereas CDKB2 is expressed specifically from the G2 to M phases [17,32]. Like Arabidopsis, the apple plant possesses two types of CDKB1 (CDKB1;1 and CDKB1;2) and two types of CDKB2 (CDKB2;1 and CDKB2;2), all of which are homologous to each other [12,17,32].…”

Section: Phylogenetic Analysis Of Cdk Genesmentioning

confidence: 99%

“…CDKB1 is expressed from the late S to M phases of the cell cycle, whereas CDKB2 is expressed specifically from the G2 to M phases [17,32]. Like Arabidopsis, the apple plant possesses two types of CDKB1 (CDKB1;1 and CDKB1;2) and two types of CDKB2 (CDKB2;1 and CDKB2;2), all of which are homologous to each other [12,17,32]. Microarray expression data from previous studies have determined that CDKB2;2 form a more distinct peak during early apple fruit development compared to CDKB2;1 [13,23].…”

Section: Phylogenetic Analysis Of Cdk Genesmentioning

confidence: 99%

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Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Karim¹,

Allan²,

Schaffer³

et al. 2022

Preprint

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Apple (Malus x domestica) fruit size is dependent on cell division and cell expansion, processes which are subsequently regulated by plant hormones such as auxins, gibberellins, and cytokinins. In this study, we investigated the role of cell division and cell expansion in apple growth and identified which of the two was more deterministic to final fruit size. Three cultivars of different sizes were selected, namely, ‘Twenty Ounce’ (large-sized), ‘Royal Gala’ (medium-sized), and ‘Crabapple’ (small-sized). Gene expression and cell size analyses were conducted over the course of two consecutive seasons. The expression patterns of three classes of genes were markedly similar across all cultivars. Two cell division markers, namely, MdCDKB2;2 and MdANT2, were discovered to be correlatively expressed as both displayed initially high expression levels, which gradually declined from the early to late stages of growth time course. For cell expansion markers, MdEXP3 was upregulated as the cells expanded, while MdARF106 was expressed in both the cell division and expansion stages. Meanwhile, the ripening related gene MdACO1 was expectedly expressed only during the ending stages associated with ripening. Interestingly, the cell measurements taken regularly from each cultivar throughout the same eperimental timespan showed that cell sizes were unaltered and remained constant from initial pollination at the zeroth Days After Pollination (DAP), to ripening at 120 Days After Full Bloom (DAFB).

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“…Here, CDKB2;2 was selected as a marker for cell division in apple fruit and cell culture. CDKA and CDKB are core cell cycle genes [17,32]. Two types of CDKBs i.e.…”

Section: Phylogenetic Analysis Of Cdk Genesmentioning

confidence: 99%

Section: Phylogenetic Analysis Of Cdk Genesmentioning

confidence: 99%

Section: Phylogenetic Analysis Of Cdk Genesmentioning

confidence: 99%

See 1 more Smart Citation

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Karim¹,

Allan²,

Schaffer³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The cell cycle consists of four main phases—G1 (pre-replicative), S (synthesis), G2 (post-replicative), and M (mitosis and cell division)—mainly controlled by the activity of cyclins, cyclin-dependent kinases (CDKs), and CDK inhibitors (CDIs) [ 59 , 60 ]. In between each of these phases there are checkpoints that evaluate whether the DNA replication was successful and decide whether the cell will continue to the next phase or if the cycle will be interrupted [ 61 , 62 ]. These cell cycle control mechanisms have been shown to have genes that can be regulated by CGs, called clock-controlled genes (CCGs) [ 63 ].…”

Section: Circadian Clock Genesmentioning

confidence: 99%

Circadian Rhythm Dysregulation and Leukemia Development: The Role of Clock Genes as Promising Biomarkers

Sanford

Cunha

Machado

et al. 2022

IJMS

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The circadian clock (CC) is a daily system that regulates the oscillations of physiological processes and can respond to the external environment in order to maintain internal homeostasis. For the functioning of the CC, the clock genes (CG) act in different metabolic pathways through the clock-controlled genes (CCG), providing cellular regulation. The CC’s interruption can result in the development of different diseases, such as neurodegenerative and metabolic disorders, as well as cancer. Leukemias correspond to a group of malignancies of the blood and bone marrow that occur when alterations in normal cellular regulatory processes cause the uncontrolled proliferation of hematopoietic stem cells. This review aimed to associate a deregulated CC with the manifestation of leukemia, looking for possible pathways involving CG and their possible role as leukemic biomarkers.

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“…Besides the recruitment at DSBs of BREAST CANCER SUSCEPTIBILITY1 (BRCA1), RADIATION SENSITIVE51 (RAD51), and RAD54 which are involved in HR (Biedermann et al, 2017;Hirakawa et al, 2017;Horvath et al, 2017), the RETINOBLASTOMA-RELATED 1 (RBR1) and the transcription factors E2Fa are also found at DSBs to promote HR (Lang et al, 2012;Biedermann et al, 2017;Horvath et al, 2017;Raynaud and Nisa, 2020). More recently, the important cell-cycle regulatory protein F-BOX-LIKE17 (FBL17) was shown to be a regulator of the DDR and to colocalize as well with RBR1 and γ-H2AX at DNA lesions, suggesting connections between cell cycle and DDR in plants (Gentric et al, 2020(Gentric et al, , 2021.…”

Section: Introductionmentioning

confidence: 99%

GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery

et al. 2022

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The maintenance of genetic information is important in eukaryotes notably through mechanisms occurring at the nuclear periphery where inner nuclear membrane proteins and nuclear pore-associated components are key factors regulating the DNA damage response (DDR). However, this aspect of DDR regulation is still poorly documented in plants. We addressed here how genomic stability is impaired in the gamma-tubulin complex component 3-interacting protein (gip1gip2) double mutants showing defective nuclear shaping. Using neutral comet assays for DNA double-strand breaks (DSBs) detection, we showed that GIP1 and GIP2 act redundantly to maintain genome stability. At the cellular level, γ-H2AX foci in gip1gip2 were more abundant and heterogeneous in their size compared to wild-type (WT) in root meristematic nuclei, indicative of constitutive DNA damage. This was linked to a constitutive activation of the DDR in the gip1gip2 mutant, with more emphasis on the homologous recombination (HR) repair pathway. In addition, we noticed the presence of numerous RAD51 foci which did not colocalize with γ-H2AX foci. The expression of GIP1-GFP in the double mutant rescued the cellular response to DNA damage, leading to the systematic colocalization of RAD51 and γ-H2AX foci. Interestingly, a significant proportion of RAD51 foci colocalized with GIP1-GFP at the nuclear periphery. Altogether, our data suggest that GIPs may partly contribute to the spatio-temporal recruitment of RAD51 at the nuclear periphery.

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Connections between the Cell Cycle and the DNA Damage Response in Plants

Cited by 16 publications

References 143 publications

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Cell division controls final fruit size in three apple (Malus x domestica) cultivars

Circadian Rhythm Dysregulation and Leukemia Development: The Role of Clock Genes as Promising Biomarkers

GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery

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