Connection of Hippocampal Neurons by Magnetically Controlled Movement of Short Electrospun Polymer Fibers—A Route to Magnetic Micromanipulators

Kriha, Olaf; Becker, Mathias; Lehmann, Martina; Kriha, Dorothee; Krieglstein, Josef; Yosef, Maekele; Schlecht, Sabine; Wehrspohn, Ralf B.; Wendorff, Joachim H.; Greiner, Andreas

doi:10.1002/adma.200601937

Cited by 73 publications

(64 citation statements)

References 17 publications

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“…55 Using an external magnetic field, dispersed fibers could undergo translational movement in water by moving the magnet horizontally, or fibers could be rotated by rotating the external magnet. The high control over fiber movement even allowed the researchers to position fibers so that a single fiber connected two primary hippocampal neurons within less than 30 seconds (Fig.…”

Section: Magnetic Field-responsive Electrospun Fibersmentioning

confidence: 99%

Stimuli-responsive electrospun fibers and their applications

et al. 2011

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In this critical review, an overview is given on recent advances in the development and application of stimuliresponsive electrospun nanofibers.Please check this proof carefully. Our staff will not read it in detail after you have returned it. Translation errors between word-processor files and typesetting systems can occur so the whole proof needs to be read. Please pay particular attention to: tabulated material; equations; numerical data; figures and graphics; and references. If you have not already indicated the corresponding author(s) please mark their name(s) with an asterisk. Please e-mail a list of corrections or the PDF with electronic notes attached --do not change the text within the PDF file or send a revised manuscript.Please bear in mind that minor layout improvements, e.g. in line breaking, table widths and graphic placement, are routinely applied to the final version.Please note that, in the typefaces we use, an italic vee looks like this: n, and a Greek nu looks like this: n.We will publish articles on the web as soon as possible after receiving your corrections; no late corrections will be made.Please return your final corrections, where possible within 48 hours of receipt, by e-mail to: chemsocrev@rsc.org Reprints-Electronic (PDF) reprints will be provided free of charge to the corresponding author. Enquiries about purchasing paper reprints should be addressed via: http://www.rsc.org/publishing/journals/guidelines/paperreprints/. Costs for reprints are below: Stimuli-responsive electrospun nanofibers are gaining considerable attention as highly versatile tools which offer great potential in the biomedical field. In this critical review, an overview is given on recent advances made in the development and application of stimuli-responsive fibers. The specific features of these electrospun fibers are highlighted and discussed in view of the properties required for the diverse applications. Furthermore, several novel biomedical applications are discussed and the respective advantages and shortcomings inherent to stimuli-responsive electrospun fibers are addressed (136 references).

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Section: Magnetic Field-responsive Electrospun Fibersmentioning

confidence: 99%

Stimuli-responsive electrospun fibers and their applications

et al. 2011

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“…180 For instance, Kriha et al has previously described the production of electrospun fibers containing MNPs whose movements can be controlled to establish connections between hippocampal neurons to facilitate neural repair. 181 Similarly, Sakar and colleagues also utilized magnetic forces to manipulate the positions of neurons with micro precision and to deliver microgels to patterned neurons. 182 Xie et al have also covalently linked nerve growth factor to magnetic nanotubes to induce differentiation in PC-12 cells.…”

Section: Discussionmentioning

confidence: 99%

Acute and Chronic Neural Stimulation via Mechano-Sensitive Ion Channels

Tay

2018

Springer Theses

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Neural stimulation techniques for eliciting calcium influx can elucidate the physiological roles of specific neural populations. To overcome some of the limitations of existing techniques such as poor specificity and noxious effects of heat, I developed a technology for non-invasive control of neural activities using magnetic forces and magnetic nanoparticles (MNPs) which offer deep tissue penetration and controllable dosage. Extensive investigations with different neurotoxins and experimental conditions support that the mechanism of magnetic stimulation that involves membrane-bound MNPs transducing magnetic forces into mechanical stretching of the cell membrane to enhance the opening probability of mechano-sensitive N-type calcium ion channels to induce calcium influx.Making use of the ability of neural networks to actively regulate their ratio of excitatory to inhibitory ion channel/receptor, I also performed chronic magnetic stimulation on fragile X iii syndrome (FXS) model neural networks. We found that chronic magnetic stimulation reduced the density of N-type calcium ion channels whose expression is increased in FXS. This technique demonstrates the potential of using bio-magnetic/mechanical forces to modulate expressions of mechano-sensitive ion channels where they are over-expressed in diseases such as abnormal nociception.Nonetheless, there are still a few areas where the technique can be improved. Firstly, it is the use of MNPs with more uniform properties to have greater control on magnetic stimulations.Secondly, the technique needs to be useful for in vivo studies. Therefore, I started researching on magnetotactic bacteria (MTB) which produce biological MNPs with superior properties such as uniform sizes and highly homogenous magnetic properties with the goal of harvesting MNPs from them.MTB, however, grow extremely slowly and the number of MNPs produced/bacterium is low. One way to overcome this problem is to evolve MTB over-producers of MNPs but this strategy is constrained by the absence of a selection platform that is quantitative and offer high throughput. To overcome this problem, I combined random chemical mutagenesis and selection using a magnetic ratcheting platform to generate and isolate MTB over-producers that produce twice as many MNPs/bacterium after 5 rounds of mutation/selection. I next designed a magnetic microfluidic device and demonstrate as a proof of concept, that it can be coupled to a bioreactor for high throughput microfluidic selection of MTB over-producers.iv

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“…In such materials different types of polymers including natural polymers, biopolymers and synthetic polymers, have been combined with magnetic nanoparticles (MNPs) including iron oxide (magnetite) (Fe3O4) [60,[104][105][106], maghemite (γ-Fe2O3) [107][108][109], cobalt (Co) [110], nickel (Ni) [111], iron-platinum (FePt) [112,113] NPs etc. From all the above, iron oxide NPs have been the most widely studied due to their biocompatibility, nontoxicity, and stability and are, by far, the most commonly employed MNPs in biomedical applications [114].…”

Section: Electrospun Magnetoactive Nanocomposites In Tissue Engineeringmentioning

confidence: 99%