Conjunctival Impression Cytology for the Diagnosis, Follow-up, and Treatment in a Patient With Severe Keratoconjunctivitis Caused by Alkali Injury

Pastor, Silvia; Rodríguez‐Prats, José Luís; Walewska-Szafran, Anna; Rodrı́guez, Alejandra; Alió, Jorge L.

doi:10.1097/ico.0b013e318190755e

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Unlike conjunctival epithelium, the normal corneal epithelium does not contain goblet cells. One way of detecting conjunctivalisation of the corneal surface is therefore to stain the sample for goblet cells (Puangsricharern and Tseng, 1995; Pastor et al, 2009). In addition, conjunctival and corneal epithelial cells differ in their cytokeratin profile.…”

Section: Limbal Stem Cell Deficiencymentioning

confidence: 99%

The culture and transplantation of human limbal stem cells

Ahmad

Osei-Bempong

Dana

et al. 2010

Journal Cellular Physiology

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The cornea is the clear front of the eye and its surface is composed of an epithelium. This is renewed by stem cells located at the limbus, which encircles the periphery of the cornea. These limbal stem cells become lost or deficient in the blinding disease of limbal stem cell deficiency. In this review article, we discuss the historical perspective in managing limbal stem cell deficiency as well as describing the more contemporary treatment options, and in particular the culture and transplantation of human limbal stem cells. This treatment was first proposed 13 years ago and many case series have been presented to date showing promising outcomes of this technique. However, challenges still remain in treating the debilitating disease of limbal stem cell deficiency. Here we discuss some of the questions, which remain to be answered in this field.

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Section: Limbal Stem Cell Deficiencymentioning

confidence: 99%

The culture and transplantation of human limbal stem cells

Ahmad

Osei-Bempong

Dana

et al. 2010

Journal Cellular Physiology

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“…Introduced by Thatcher et al [1] and Egbert et al [2], IC is an easy-to-use technique of directly retrieving (mainly conjunctival) cells, using various filter types which are gently applied to exposed (interpalpebral) conjunctival surfaces. The filters used include cellulose acetate [3], polycarbonate (Biopore, Millipore UK Ltd, Watford, UK) [4], nitrocellulose [5] or polyethersulfone (PES) [6] which are gently applied to exposed (interpalpebral) conjunctival surfaces. Recently, a more standardized tool for IC sampling has been introduced (Figures 1 & 2).…”

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confidence: 99%

Biomarkers of Ocular Surface Disease Using Impression Cytology

Hagan

2017

Biomark. Med.

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Impression cytology (IC) is a technique which permits the retrieval of the outermost layer of ocular surface cells via the use of various types of filters. It is a minimally invasive method of evaluating human conjunctival epithelial cell morphology in the diagnosis of dry eye disease, a common and distressing disorder associated with ageing, contact lens wear, autoimmune disorders and refractive (LASIK) surgery. IC may also be utilized in the diagnosis of other ocular diseases, such as keratoconus and thyroid orbitopathy. More recently, IC has been utilized for the subsequent investigation of gene and protein expression of conjunctival cells in order to identify novel diagnostic biomarkers and to further our understanding of the mechanisms underlying ocular surface disease. This review will therefore examine the literature concerning the role of IC in identifying cellular markers of eye disease, systemic diseases with ocular involvement and potential novel therapeutic targets. Impression cytology (IC) is a minimally invasive method of evaluating human ocular surface cells and is considered as the 'gold standard' technique for assessing cell morphology. Introduced by Thatcher et al. [1] and Egbert et al. [2], IC is an easy-to-use technique of directly retrieving (mainly conjunctival) cells, using various filter types which are gently applied to exposed (interpalpebral) conjunctival surfaces. The filters used include cellulose acetate [3], polycarbonate (Biopore, Millipore UK Ltd, Watford, UK) [4], nitrocellulose [5] or polyethersulfone (PES) [6] which are gently applied to exposed (interpalpebral) conjunctival surfaces. Recently, a more standardized tool for IC sampling has been introduced (Figures 1 & 2). The Eyeprim is a new single-use PES filter sampling medical device for retrieval of conjunctival cells (Opia Technologies, France) and a recent study by Lopez-Miguel et al. [7] performed an analysis of this tool versus the traditional IC method (PES). On a cohort of 20 healthy individuals, they extracted and compared RNA from both devices and found no significant differences in the mean yield (p ≥ 0.45), the total RNA amount (μg), or the amount normalized to membrane area [7]. Although an earlier study of the Eyeprim [8], suggested that it recovered more cells than traditional IC, Lopez-Miguel et al. [7] did not observe differences in the RNA collected between this device and PES.Historically, IC has been used to analyze pathological changes to conjunctival cells for indicators of squamous metaplasia in ocular surface disease (OSD) [9]. More recently, however, researchers have used IC to examine specific markers of inflammation, for example, HLA-DR, ICAM-1 and CD8 [10][11][12], and to examine novel biomarkers of dry eye disease (DED). The latter is performed in an attempt to understand the mechanisms underpinning DED pathophysiology and is now feasible, due to vast improvements in the assay sensitivities for gene and protein examination [13]. As a result, IC membranes can be subsequently analyzed using techniq...

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Conjunctival Impression Cytology for the Diagnosis, Follow-up, and Treatment in a Patient With Severe Keratoconjunctivitis Caused by Alkali Injury

Cited by 2 publications

References 12 publications

The culture and transplantation of human limbal stem cells

The culture and transplantation of human limbal stem cells

Biomarkers of Ocular Surface Disease Using Impression Cytology

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