Conjunctival cytology assessment in dogs and cats. Sampling, diagnostic techniques and findings

Athanasiou, Labrini V.; Psemmas, D. Ε.; Papaioannou, N.

doi:10.12681/jhvms.16382

Cited by 9 publications

(18 citation statements)

References 16 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of binucleated cells is considered as an indicator of reactive changes in areas of severe chronic in ammation. On the other hand, the cytological analysis of the sample taken from the left eye of the same animal demonstrated normal ocular surface cells, similar to descriptions made in previous literature reports (Bolzan et al 2005;Athanasiou et al 2018). In the present study, based on histopathological examination (Fig.…”

Section: Discussionsupporting

confidence: 89%

“…In agreement with the ndings of previous reports for cytospin slides prepared from samples of animals with keratoconjunctivitis(Cakir et al 2014), the present study revealed the presence of binucleated epithelial cells.The presence of binucleated cells is considered as an indicator of reactive changes in areas of severe chronic in ammation. On the other hand, the cytological analysis of the sample taken from the left eye of the same animal demonstrated normal ocular surface cells, similar to descriptions made in previous literature reports(Bolzan et al 2005;Athanasiou et al 2018). In the present study, based on histopathological examination…”

supporting

confidence: 91%

See 1 more Smart Citation

Panophthalmitis in a Gentoo Penguin (Pygoscelis Papua) From The Antarctic Peninsula: Evaluation of Microbiological and Histopathological Analysis Outcomes

Bayram¹,

Abay²,

Satıcıoğlu³

et al. 2021

Preprint

View full text Add to dashboard Cite

In this study, it was aimed to present the results of microbiological, cytological, histopathological, and immunohistochemical analyses of ocular samples from an Antarctic (Ardley Island, King George Island) Gentoo penguin chick (Pygoscelis papua) with pyogranulomatous panophthalmitis in the right eye. Samples were taken from both the healthy left eye and the lesion in the right eye. Conventional culture methods and phenotypic and molecular tests were used for bacterial isolation and identification, respectively. None of the isolates could be identified phenotypically. As a result, four of the five isolates obtained from the right eye were considered to belong to putative novel bacterial species and taxa as their similarity to GenBank data was below 98.75%. The isolates were considered to be Pasteurellaceae bacterium, Corynebacterium ciconiae, Cardiobacteriaceae bacterium, Actinomyces sp., and Dermabacteraceae bacterium. The only isolate from the left eye was identified as Psychrobacter pygoscelis. The cytological analysis demonstrated cell infiltrates composed mostly of degenerate heterophils, reactive macrophages, plasma cells, lymphocytes, and eosinophils. Based on histopathological findings, the lesion was defined as typical pyogranulomatous panophthalmitis. Immunohistochemistry demonstrated that the granuloma was positive for TNF-α, IL-4, MMP-9, IL-1β, and IL-6. This is the first documented report of unilateral pyogranulomatous panophthalmitis in a Gentoo penguin chick, living in its natural habitat in Antarctica. This report also describes the isolation of four bacteria from the infected eye, which are considered to belong to novel Genus, species, or taxa. The primary bacterial pathogen that caused the ocular lesion was not able to be detected and remains unclear.

show abstract

Section: Discussionsupporting

confidence: 89%

supporting

confidence: 91%

Panophthalmitis in a Gentoo Penguin (Pygoscelis Papua) From The Antarctic Peninsula: Evaluation of Microbiological and Histopathological Analysis Outcomes

Bayram¹,

Abay²,

Satıcıoğlu³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The slide was air-dried and stained using Dip Quik (JorVet, USA). Evaluator I (2 nd author) mostly had theoretical experience in ocular cytology that comprised reviewing of books, pictures, atlases, and online materials [2,[5][6][7][8]. Evaluator II (1 st author) had undergone clinical pathology residency training and had 16 years of practical experience.…”

Section: Animals and Samplingmentioning

confidence: 99%

“…Ophthalmological examination of varying complexity is performed by veterinarians in general practice and veterinary ophthalmologists [1,2]. Microscopic examination is relatively easy to perform, but the usefulness of this test is affected by the sample quality, such as the cellularity, cell preservation, and the evaluator's experience [3,4].…”

Section: Introductionmentioning

confidence: 99%

Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators

2022

View full text Add to dashboard Cite

Background and Aim: Cytology investigations are a frequent part of ophthalmological examination. We aimed to assess whether the cytological findings of healthy and conjunctivitis/keratoconjunctivitis samples differed based on the evaluator's experience. Materials and Methods: A study evaluated healthy eyes (n = 40) and eyes affected with keratoconjunctivitis and/ or conjunctivitis (n = 28) in dogs. An ophthalmological examination was performed before sampling the eyes using a sterile cotton swab. An evaluator with theoretical experience and one with undergone clinical pathology residency training performed cytology blinded to the clinical findings. Results: In the healthy eyes group, the agreement between the evaluators for cellularity was nonexistent, while that for cell preservation and mucus content was fair. In the affected eyes group, the agreement for cellularity and mucus content was moderate, while that for cell preservation was fair. The inadequate sample rate differed significantly between the two evaluators in the healthy eyes group (p = 0.006) but not in the affected eyes group (p = 0.083). Bacterial presence was detected by both evaluators, and the findings did not differ statistically from the bacteriology results (p = 0.05). Significant variations were noted in the differential cell count; the mean count of the superficial epithelial cells and goblet cells of the healthy eyes group (p < 0.05) and that of the basal/intermediate cells and neutrophils of the affected eyes (p < 0.05) showed significant differences. Conclusion: The evaluator's experience significantly affected the differential cell count in both the healthy and affected eyes groups. Neutrophil degeneration was not observed by the less experienced evaluator, whereas bacteria were detected equally well by both the evaluators.

show abstract

“…The use of noninvasive sampling techniques is of interest, especially in feline medicine (Athanasiou et al, 2018). Recently Leishmania DNA was detected from conjunctival (Benassi et al, 2017) and oral swabs of cats (Persichetti et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Serological study of feline leishmaniasis and molecular detection of Leishmania infantum and Leishmania braziliensis in cats (Felis catus)

Costa-Val

Coura

Barbieri

et al. 2020

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Abstract Blood samples and swabs from ocular conjunctiva and mouth were obtained from 64 cats. Of 64 serum samples, 19 were positive for Leishmania antibodies by ELISA (29.80%). Eight cats were positive by PCR (12.5%) in swab samples from mouth and/or ocular mucosa. Poor kappa agreement between serological and molecular results (k = 0.16) was obtained. From five positive PCR samples one was L. braziliensis and four were L. infantum. Phylogenetic analysis performed with the five isolates of Leishmania, showed that samples of L. infantum isolated from the cats were phylogenetically close to those isolated from domestic dogs in Brazil, while the L. braziliensis is very similar to the one described in humans in Venezuela. The study demonstrated that, despite high seropositivity for Leishmania in cats living in the study region, poor agreement between serological and molecular results indicate that positive serology is not indicative of Leishmania infection in cats. Parasite DNA can be detected in ocular conjunctiva and oral swabs from cats, indicating that such samples could be used for diagnosis. Results of phylogenetic analyzes show that L. infantum circulating in Brazil is capable of infecting different hosts, demonstrating the parasite's ability to overcome the interspecies barrier.

show abstract

Conjunctival cytology assessment in dogs and cats. Sampling, diagnostic techniques and findings

Cited by 9 publications

References 16 publications

Panophthalmitis in a Gentoo Penguin (Pygoscelis Papua) From The Antarctic Peninsula: Evaluation of Microbiological and Histopathological Analysis Outcomes

Panophthalmitis in a Gentoo Penguin (Pygoscelis Papua) From The Antarctic Peninsula: Evaluation of Microbiological and Histopathological Analysis Outcomes

Difference in cytological findings of healthy and conjunctivitis/keratoconjunctivitis affected canine eyes between variably experienced evaluators

Serological study of feline leishmaniasis and molecular detection of Leishmania infantum and Leishmania braziliensis in cats (Felis catus)

Contact Info

Product

Resources

About