Conidium production by insect pathogenic fungi on commercially available agars

Abstract

Aims: Conidium production by three species of insect pathogenic fungi, Metarhizium anisopliae , Beauveria bassiana and Verticillium lecanii , was assessed on various depths and types of commercially available agars. Methods: Conidium production was assessed after 14 d of growth on commercially available media as well as at three different agar depths. Results: Metarhizium anisopliae and B. bassiana isolates showed greatest conidium production on potato dextrose agar (PDA) at a depth of 2 mm, whereas V. lecanii showed greatest conidium production on yeast extract‐peptone‐dextrose agar (YPDA) regardless of agar depth. Optimum conidium production for M. anisopliae and B. bassiana was not only dependent upon the isolate used but also on the medium type and agar depth. Significance and Impact of the Study: Conidia are the infective structures for insect pathogenic fungi and this study suggests a rationale basis for consistent conidium production for laboratory and commercial practices.

Effect of moisture content and inoculum on the growth and conidia production by Beauveria bassiana on wheat bran

“…The values for radial extension rate markedly enhanced (between 10 and 15%) after the addition of liquid media reported by Alves et al (2002) or a standard Dextrose Sabouraud broth. This behavior was similar to that reported by Kamp and Bidochka (2002) who found better growth for B. bassiana in more complex media. Our B. bassiana strain was very sensitive to variation in water activity (a w ) since radial extension rate decreased from 77 µm/h (at a w = 1.0) down to 15 µm/h (at a w = 0.981) in sole wheat bran medium (Fig.…”

Newly Isolated Paecilomyces lilacinus and Paecilomyces javanicus as Novel Biocontrol Agents for Plutella xylostella and Spodoptera litura

“…A control industrial residues or agricultural products is a great alternative to achieve a competitive price, resulting in the utilization of agro-industrial residues with added value (Soccol and Vandenberghe, 2003). Sorghum, white rice, wheat, coffee husks, rye, barley, sugarcane, cassava bagasse, refused potatoes, corn, beans, soy, glucose syrup, and grapes have been used as materials for spore production (Buzzini and Martini, 1999;Pandey et al, 2000;Kamp and Bidochka, 2002;Santa et al, 2005;Robl et al, 2009;Mishra and Thawani, 2016). Solidstate fermentation of these crop residues is advantageous because it is easy to carry out, and raw materials are cheap (Mishra and Thawani, 2016).…”

Identification of novel genes associated with conidiation inBeauveria bassianawith suppression subtractive hybridization

“…A better understanding of the genetics of conidiation in Beauveria may assist in consistent production of conidia. Most research has been focused on production method development and fermentation optimization to enhance sporulation (Wraight et al 2001, Kemp andBidochka 2002). Little is known about the mechanism of sporulation in B. bassiana or the genes involved in conidiation.…”