Identification of novel genes associated with conidiation in<i>Beauveria bassiana</i>with suppression subtractive hybridization

Wu, Jiang; Ridgway, Hayley J.; Carpenter, Margaret A.; Glare, Travis R.

doi:10.1080/15572536.2008.11832496

Cited by 5 publications

(1 citation statement)

References 45 publications

(10 reference statements)

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“…Investigation on this fungus covered the molecular basis of development and virulence has extended our knowledge of fungus-insect interaction mechanism in recent years [6]. Moreover, several genetic transformation systems [7] and gene knockout techniques have been developed in this species to facilitate the study of gene function in vivo.…”

Section: Introductionmentioning

confidence: 99%

Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans

Hasan

Singh

2011

Indian J Microbiol

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A heterologous transformation system was developed for V. lecanii based on the complementation of a nitrate reductase mutant. Nitrate reductase mutants were obtained by resistance to chlorate in a rate of 23.24% when compared to other mutations that lead to the chlorate resistance. Mutant no. 01 and 04 was chosen for the transformation experiments. Plasmid pBT was used as transformation vector containing the Aspergillus nidulans nitrate reductase gene. A frequency of approximately 3 transformants/lg DNA was obtained using the circular vector pBT. The establishment of a transformation system for V. lecanii is fundamental for genetic manipulation of this microorganism.

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Section: Introductionmentioning

confidence: 99%

Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans

Hasan

Singh

2011

Indian J Microbiol

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Comparative Transcriptomic and Proteomic Analyses Reveal a FluG‐Mediated Signaling Pathway Relating to Asexual Sporulation of Antrodia camphorata

Lü

Zhu

et al. 2017

Proteomics

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Medicinal mushroom Antrodia camphorata sporulate large numbers of arthroconidia in submerged fermentation, which is rarely reported in basidiomycetous fungi. Nevertheless, the molecular mechanisms underlying this asexual sporulation (conidiation) remain unclear. Here, we used comparative transcriptomic and proteomic approaches to elucidate possible signaling pathway relating to the asexual sporulation of A. camphorata. First, 104 differentially expressed proteins and 2586 differential cDNA sequences during the culture process of A. camphorata were identified by 2DE and RNA-seq, respectively. By applying bioinformatics analysis, a total of 67 genes which might play roles in the sporulation were obtained, and 18 of these genes, including fluG, sfgA, SfaD, flbA, flbB, flbC, flbD, nsdD, brlA, abaA, wetA, ganB, fadA, PkaA, veA, velB, vosA, and stuA might be involved in a potential FluG-mediated signaling pathway. Furthermore, the mRNA expression levels of the 18 genes in the proposed FluG-mediated signaling pathway were analyzed by quantitative real-time PCR. In summary, our study helps elucidate the molecular mechanisms underlying the asexual sporulation of A. camphorata, and provides also useful transcripts and proteome for further bioinformatics study of this valuable medicinal mushroom.

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Identification of genes that are preferentially expressed in conidiogenous cell development of Metarhizium anisopliae by suppression subtractive hybridization

Peng

Xie

et al. 2009

Curr Genet

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The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a powerful strategy for obtaining insight into the molecular events that regulate conidiation. We isolated genes that are preferentially expressed in the developing conidiophores of the common fungal locust pathogen M. anisopliae CQMa102 using suppression subtractive hybridization. Based on the results of cDNA array dot blotting, we identified 109 unique expressed sequence tags (ESTs) that were up-regulated more than fivefold during conidiophore formation. Among these 109 ESTs were 45 (41.3%) with significant similarity to NCBI annotated hypothetical proteins, 35 (32.1%) with low similarity to known or predicted genes that might represent novel genes, and 29 (26.6%) with significant similarity to known proteins involved in various cell and molecular processes, such as ell structure and function, cell metabolism, protein metabolism, stress response, nucleic acid metabolism, and cell cycle and growth. We confirmed the up-regulation of 11 randomly selected genes with real-time reverse transcriptase-PCR analysis. The results of this study provide a preliminary description of genes that may be involved in the molecular regulation of fungal conidiogenesis.

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Identification of novel genes associated with conidiation inBeauveria bassianawith suppression subtractive hybridization

Cited by 5 publications

References 45 publications

Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans

Development of Transformation System of Verticillium lecanii (Lecanicillium spp.) (Deuteromycotina: Hyphomycetes) Based on Nitrate Reductase Gene of Aspergillus nidulans

Comparative Transcriptomic and Proteomic Analyses Reveal a FluG‐Mediated Signaling Pathway Relating to Asexual Sporulation of Antrodia camphorata

Identification of genes that are preferentially expressed in conidiogenous cell development of Metarhizium anisopliae by suppression subtractive hybridization

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