Congenital toxoplasmosis: the importance of implementing clinical practice guidelines

Amendoeira, Maria Regina Reis; Millar, Patricia Riddell

doi:10.1016/j.lana.2021.100023

Cited by 3 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7,8 The importance of diagnosing active infection by T. gondii during pregnancy and of confirming congenital transmission in newborns (NBs) cannot be overstated, because it allows for the adoption of measures of primary and secondary care, minimizing serious impairments caused by congenital transmission. 9 In the routine laboratory tests offered by the Brazilian Unified Health System (SUS, in the Portuguese acronym), toxoplasmosis is diagnosed by means of serological tests based on the detection of specific antibodies of the classes of immunoglobulin M (IgM) and immunoglobulin G (IgG), mainly by means of the enzyme-linked immunosorbent assay (ELISA) method. 10 However, assistance provided to pregnant women during prenatal care is still not satisfactory.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IgG Avidity in Samples Collected on Filter Paper: Importance of The Early Diagnosis of Congenital Toxoplasmosis

Souza

Gomes

Rezende

et al. 2021

Rev Bras Ginecol Obstet

View full text Add to dashboard Cite

Objective The purpose of the present study is to standardize and evaluate the use of the immunoglobulin G (IgG) antibody avidity test on blood samples from newborns collected on filter paper to perform the heel test aiming at its implementation in ongoing programs. Methods Blood samples from newborns were collected on filter paper simultaneously with the heel prick test. All samples were subjected to immunoglobulin M IgM and IgG enzyme-linked immunosorbent assays (ELISA). Peripheral blood was collected again in the traditional way and on filter paper from newborns with high IgG levels (33). Three types of techniques were performed, the standard for measuring IgG in serum, adapted for filter paper and the technique of IgG avidity in serum and on filter paper. The results of the avidity test were classified according to the Rahbari protocol. Results Among the 177 samples, 17 were collected in duplicate from the same child, 1 of peripheral blood and 1 on filter paper. In this analysis, 1 (5.88%) of the 17 samples collected in duplicate also exhibited low IgG avidity, suggesting congenital infection. In addition, the results obtained from serum and filter paper were in agreement, that is, 16 (94.12%) samples presented high avidity, with 100% agreement between the results obtained from serum and from filter paper. Conclusion The results of the present study indicate that the avidity test may be another valuable method for the diagnosis of congenital toxoplasmosis in newborns.

show abstract

Section: Introductionmentioning

confidence: 99%

“…The importance of diagnosing active infection by T. gondii during pregnancy and of confirming congenital transmission in newborns (NBs) cannot be overstated, because it allows for the adoption of measures of primary and secondary care, minimizing serious impairments caused by congenital transmission. 9 …”

Section: Introductionmentioning

confidence: 99%