Conformational changes in human plasma proteins induced by metal oxide nanoparticles

Simón‐Vázquez, Rosana; Lozano‐Fernández, Tamara; Olmedo, Mercedes Peleteiro; González-Fernández, África

doi:10.1016/j.colsurfb.2013.08.047

Cited by 55 publications

(64 citation statements)

References 47 publications

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“…In contrast, a slight red region-shift on the emission band of the globulins suggests an adsorption of these proteins exclusively on the MIL-100(Fe) surface, which may exhibit a more unfolded conformation with more exposed tryptophan residues. [ 58 ] Interestingly, transferrin, an iron-binding blood plasma glycoprotein belonging to the globulin family, could be the responsible of this slight conformational change, only visible at high NP concentration (100 µg mL −1 ), as a consequence of their interaction with iron. Conversely, the heparin-coated MIL-100(Fe) NPs did not show any signifi cant shift, in agreement with the absence of conformational changes of globulins.…”

Section: Uptake By Macrophages Cell Viability Ros Production and Cmentioning

confidence: 99%

“…As recently shown by some of us, NPs can induce conformational changes on proteins, altering also their function. [ 58 ] To gain further understanding, we investigated if upon adsorption of the main plasma proteins (fi brinogen, albumin, and globulins) on both the uncoated and heparin-coated NPs, the proteins could undergo conformational changes. Thus, both NPs were incubated in presence of increasing concentration of each protein fraction, analyzing the conformational changes by using fl uorescence spectroscopy (see Experimental Section).…”

Section: Uptake By Macrophages Cell Viability Ros Production and Cmentioning

confidence: 99%

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Heparin‐Engineered Mesoporous Iron Metal‐Organic Framework Nanoparticles: Toward Stealth Drug Nanocarriers

Bellido

Hidalgo

Lozano

et al. 2015

Adv Healthcare Materials

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191

152

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The specific modification of the outer surface of the promising porous metal-organic framework nanocarriers (nanoMOFs) preserving their characteristic porosity is still a major challenge. Here a simple, fast, and biofriendly method for the external functionalization of the benchmarked mesoporous iron(III) trimesate nanoparticles MIL-100(Fe) with heparin, a biopolymer associated with longer-blood circulation times is reported. First, the coated nanoparticles showed intact crystalline structure and porosity with improved colloidal stability under simulated physiological conditions, preserving in addition its encapsulation and controlled release capacities. The effect of the heparin coating on the nanoMOF interactions with the biological environment is evaluated through cell uptake, cytotoxicity, oxidative stress, cytokine production, complement activation, and protein adsorption analysis. These results confirmed that the heparin coating endowed the nanoMOFs with improved biological properties, such as reduced cell recognition, lack of complement activation, and reactive oxygen species production. Overall, the ability to coat the surface of the nanoMOFs using a simple and straight-forward approach could be taken as a way to enhance the versatility and, thus, the potential of porous MOF nanoparticles in biomedicine.

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Section: Uptake By Macrophages Cell Viability Ros Production and Cmentioning

confidence: 99%

Section: Uptake By Macrophages Cell Viability Ros Production and Cmentioning

confidence: 99%

Heparin‐Engineered Mesoporous Iron Metal‐Organic Framework Nanoparticles: Toward Stealth Drug Nanocarriers

Bellido

Hidalgo

Lozano

et al. 2015

Adv Healthcare Materials

Self Cite

191

152

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“…The Np size was determined by high-resolution transmission electron microscopy, and all of the Nps were uncoated and free of surfactant. The transmission electron microscopy images and dynamic light scattering characterization have been described previously 26 and they are shown in Figure S1. The Nps were resuspended in milli-Q water at 10 mg/mL and, after sonication, 10% fetal bovine serum was added to reduce the aggregation of the Nps.…”

Section: Introductionmentioning

confidence: 99%

Metal oxide nanoparticles interact with immune cells and activate different cellular responses

Simón‐Vázquez¹,

Lozano‐Fernández²,

Dávila-Grana³

et al. 2016

IJN

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Besides cell death, nanoparticles (Nps) can induce other cellular responses such as inflammation. The potential immune response mediated by the exposure of human lymphoid cells to metal oxide Nps (moNps) was characterized using four different moNps (CeO 2 , TiO 2 , Al 2 O 3 , and ZnO) to study the three most relevant mitogen-activated protein kinase subfamilies and the nuclear factor kappa-light-chain-enhancer of the activated B-cell inhibitor, IκBα, as well as the expression of several genes by immune cells incubated with these Nps. The moNps activated different signaling pathways and altered the gene expression in human lymphocyte cells. The ZnO Nps were the most active and the release of Zn 2+ ions was the main mechanism of toxicity. CeO 2 Nps induced the smallest changes in gene expression and in the IκBα protein. The effects of the particles were strongly dependent on the type and concentration of the Nps and on the cell activation status prior to Np exposure.

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“…Also, whether the particles are agglomerated or not on the biological fluids [33,101] could strongly modify their uptake. Moreover, nanoparticles can induce conformational changes in proteins, affecting their physiological function [147]. Thus, in vivo fate of nanostructures and the biological responses could be altered when compared with in vitro studies.…”

Section: Protein Coronamentioning

confidence: 99%

“…Possible nanostructure-cellular interactions may induce cytotoxicity and other physio-logical outcomes to GI tract. For example, oxidative stress, inflammation and immune responses (phagocytosis, complement activation and recruitment of inflammatory cells) [187], mitochondrial perturbation, ''corona'' formation (please see ''Protein corona'' section), protein denaturation [147], DNA damage and cell death [33,81,97]. Although some of these responses could be beneficial for some purposes, such as the activation of specific immune responses by nanostructures carrying antigens on vaccines [128,168], some of those could have harmful effects.…”

Section: Nanotoxicity Assessment In Gi Tractmentioning

confidence: 99%

Edible Bio-Based Nanostructures: Delivery, Absorption and Potential Toxicity

et al. 2015

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The development of bio-based nanostructures as nanocarriers of bioactive compounds to specific body sites has been presented as a hot topic in food, pharmaceutical and nanotechnology fields. Food and pharmaceutical industries seek to explore the huge potential of these nanostructures, once they can be entirely composed of biocompatible and non-toxic materials. At the same time, they allow the incorporation of lipophilic and hydrophilic bioactive compounds protecting them against degradation, maintaining its active and functional performance. Nevertheless, the physicochemical properties of such structures (e.g., size and charge) could change significantly their behavior in the gastrointestinal (GI) tract. The main challenges in the development of these nanostructures are the proper characterization and understanding of the processes occurring at their surface, when in contact with living systems. This is crucial to 2 understand their delivery and absorption behavior as well as to recognize potential toxicological effects. This review will provide an insight into the recent innovations and challenges in the field of delivery via GI tract using bio-based nanostructures. Also, an overview of the approaches followed to ensure an effective deliver (e.g., avoiding physiological barriers) and to enhance stability and absorptive intestinal uptake of bioactive compounds will be provided. Information about nanostructures' potential toxicity and a concise description of the in vitro and in vivo toxicity studies will also be given.

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Conformational changes in human plasma proteins induced by metal oxide nanoparticles

Cited by 55 publications

References 47 publications

Heparin‐Engineered Mesoporous Iron Metal‐Organic Framework Nanoparticles: Toward Stealth Drug Nanocarriers

Heparin‐Engineered Mesoporous Iron Metal‐Organic Framework Nanoparticles: Toward Stealth Drug Nanocarriers

Metal oxide nanoparticles interact with immune cells and activate different cellular responses

Edible Bio-Based Nanostructures: Delivery, Absorption and Potential Toxicity

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