Metal oxide nanoparticles interact with immune cells and activate different cellular responses

Simón‐Vázquez, Rosana; Lozano‐Fernández, Tamara; Dávila-Grana, Angela; González-Fernández, África

doi:10.2147/ijn.s110465

Cited by 31 publications

(25 citation statements)

References 43 publications

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“…Cultured cells were exposed to different concentrations of APS-MNPs (ranging from 10 to 500 µg Fe/ml) for 24 h. At all tested concentrations viability was over 100% compared to untreated cells maintained at standard culture conditions, although no statistically significant differences were found. This result suggests a slight induction of T cell proliferation by MNPs, as described for other metal oxide nanoparticles 65 . .…”

Section: Influence Of Subcellular Localization On Mhtsupporting

confidence: 67%

Cell-Promoted Nanoparticle Aggregation Decreases Nanoparticle-Induced Hyperthermia under an Alternating Magnetic Field Independently of Nanoparticle Coating, Core Size, and Subcellular Localization

Mejías

Flores

Talelli

et al. 2018

ACS Appl. Mater. Interfaces

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Magnetic hyperthermia has a significant potential to be a new breakthrough for cancer treatment. The simple concept of nanoparticle-induced heating by the application of an alternating magnetic field has attracted much attention, as it allows the local heating of cancer cells, which are considered more susceptible to hyperthermia than healthy cells, while avoiding the side effects of traditional hyperthermia. Despite the potential of this therapeutic approach, the idea that local heating effects due to the application of alternating magnetic fields on magnetic nanoparticle-loaded cancer cells can be used as a treatment is controversial. Several studies indicate that the heating capacity of magnetic nanoparticles is largely reduced in the cellular environment due to increased viscosity, aggregation and dipolar interactions. However, an increasing number of studies, both in vitro and in vivo, show evidence of successful magnetic hyperthermia treatment on several different types of cancer cells. This apparent contradiction might be due to the use of different experimental conditions. Here we analyze the effects of several parameters on the cytotoxic efficiency of magnetic nanoparticles as heat inductors under an alternating magnetic field. Our results indicate that cell-nanoparticle interaction reduces the cytotoxic effects of magnetic hyperthermia, independently of nanoparticle coating and core size, the cell line used and the subcellular localization of nanoparticles. However, there seems to occur a synergistic effect between the application of an external source of heat and the presence of magnetic nanoparticles, leading to higher toxicities than those induced by heat alone, or the accumulation of nanoparticles within cells.

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Section: Influence Of Subcellular Localization On Mhtsupporting

confidence: 67%

Cell-Promoted Nanoparticle Aggregation Decreases Nanoparticle-Induced Hyperthermia under an Alternating Magnetic Field Independently of Nanoparticle Coating, Core Size, and Subcellular Localization

Mejías

Flores

Talelli

et al. 2018

ACS Appl. Mater. Interfaces

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“…Nevertheless, other studies have shown that MNPs can induce some intracellular activation markers in certain cases [61,62], although this depends mainly on NP design [63].…”

Section: Dicussionmentioning

confidence: 92%

Magnetic targeting of adoptively transferred tumour-specific nanoparticle-loaded CD8+ T cells does not improve their tumour infiltration in a mouse model of cancer but promotes the retention of these cells in tumour-draining lymph nodes

et al. 2019

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Background: Adoptive T cell-transfer (ATC) therapy is a highly promising cancer-treatment approach. However, in vivo-administered T cells tend to disperse, with only a small proportion reaching the tumour. To remedy this, magnetic targeting of T cells has been recently explored. Magnetic nanoparticles (MNPs) functionalised with antibodies were attached to effector T cells and magnetically recruited to tumour sites under MRI guidance. In this study, we investigated whether 3-aminopropyl-triethoxysilane (APS)-coated MNPs directly attached to CD8 + T cell membranes could also magnetically target and accumulate tumour-specific CD8 + T cells in solid tumours using an external magnetic field (EMF). As it has been shown that T cells associated with APS-coated MNPs are retained in lymph nodes (LNs), and tumour-draining LNs are the most common sites of solid-tumour metastases, we further evaluated whether magnetic targeting of APS-MNP-loaded CD8 + T cells could cause them to accumulate in tumour-draining LNs. Results: First, we show that antigen-specific CD8 + T cells preserve their antitumor activity in vitro when associated with APS-MNPs. Next, we demonstrate that the application of a magnetic field enhanced the retention of APS-MNPloaded OT-I CD8 + T cells under flow conditions in vitro. Using a syngeneic mouse model, we found similar numbers of APS-MNP-loaded OT-I CD8 + T cells and OT-I CD8 + T cells infiltrating the tumour 14 days after cell transfer. However, when a magnet was placed near the tumour during the transfer of tumour-specific APS-MNP-loaded CD8 + T cells to improve tumour infiltration, a reduced percentage of tumour-specific T cells was found infiltrating the tumour 14 days after cell transfer, which was reflected in a smaller reduction in tumour size compared to tumour-specific CD8 + T cells transferred with or without MNPs in the absence of a magnetic field. Nonetheless, magnet placement near the tumour site during cell transfer induced infiltration of activated tumour-specific CD8 + T cells in tumour-draining LNs, which remained 14 days after cell transfer. Conclusions: The use of an EMF to improve targeting of tumour-specific T cells modified with APS-MNPs reduced the percentage of these cells infiltrating the tumour, but promoted the retention and the persistence of these cells in the tumour-draining LNs.

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“…Preliminary tests conducted in our laboratory on Jurkat cells proved that ZnO (50 and 100 µg/mL) causes the activation of three mitogen-activated protein kinases (MAPKs), thus inducing the phosphorylation of p38, ERK 1/2 and SAPK/JNK, together with the degradation of IκBα, the NFκB inhibitor. In contrast, Al 2 O 3 , CeO 2 and TiO 2 only showed activation of some of these routes and the degradation of IκBα at high concentrations (100 µg/mL) [ 24 ]. Moreover, Jurkat cells were more sensitive to activation of these signalling proteins when compared to NCI-H460 cells [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…In contrast to Jurkat cells, where only ERK1/2 and p38 were activated by CeO 2 Nps mainly after pre-stimulation of the cells, in human hepatoma cells the SAPK/JNK was also activated by these Nps and activation of the three MAPKs was associated with oxidative stress and reduced cell viability at concentrations ≥50 µg/mL [ 27 ]. Interestingly, TiO 2 Nps induced activation of ERK1/2 and p38 in a human neutrophil [ 29 ] and a bronchial epithelial cell line [ 30 ] as described for Jurkat cells [ 24 ]. However, the activation of these signalling proteins was associated with the inhibition of apoptosis in the lymphocytic and neutrophil cell line, while in the bronchial epithelial cells the Nps induced apoptosis and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Metal Oxide Nanoparticles on Cell Viability and Activation of MAP Kinases and NFκB

Dávila-Grana

Diego-González

González-Fernández

et al. 2018

IJMS

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In recent years, there has been an increase in the production of several types of nanoparticles (Nps) for different purposes. Several studies have been performed to analyse the toxicity induced by some of these individual Nps, but data are scarce on the potential hazards or beneficial effects induced by a range of nanomaterials in the same environment. The purpose of the study described here was to evaluate the toxicological effects induced by in vitro exposure of human cells to ZnO Nps in combination with different concentrations of other metal oxide Nps (Al2O3, CeO2, TiO2 and Y2O3). The results indicate that the presence of these Nps has synergistic or antagonistic effects on the cell death induced by ZnO Nps, with a quite marked beneficial effect observed when high concentrations of Nps were tested. Moreover, analysis by Western blot of the main components of the intracellular activation routes (MAPKs and NFκB) again showed that the presence of other Nps can affect cell activation. In conclusion, the presence of several Nps in the same environment modifies the functional activity of one individual Np. Further studies are required in order to elucidate the effects induced by combinations of nanomaterials.

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Metal oxide nanoparticles interact with immune cells and activate different cellular responses

Cited by 31 publications

References 43 publications

Cell-Promoted Nanoparticle Aggregation Decreases Nanoparticle-Induced Hyperthermia under an Alternating Magnetic Field Independently of Nanoparticle Coating, Core Size, and Subcellular Localization

Cell-Promoted Nanoparticle Aggregation Decreases Nanoparticle-Induced Hyperthermia under an Alternating Magnetic Field Independently of Nanoparticle Coating, Core Size, and Subcellular Localization

Magnetic targeting of adoptively transferred tumour-specific nanoparticle-loaded CD8+ T cells does not improve their tumour infiltration in a mouse model of cancer but promotes the retention of these cells in tumour-draining lymph nodes

Synergistic Effect of Metal Oxide Nanoparticles on Cell Viability and Activation of MAP Kinases and NFκB

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