Conformation changes of albumin and lysozyme on electrospun TiO2 nanofibers and its effects on MSC behaviors

Zhao, Fenghua; Chen, Y.M.; Hu, Yanyan; Lu, Xiong Gang; Xiong, Shibing; Wu, Boyao; Guo, Yang; Huang, Ping; Yang, Bang Cheng

doi:10.1016/j.colsurfb.2019.110604

Cited by 10 publications

(8 citation statements)

References 37 publications

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“…HSA is in general a soft protein. Experimentally it was found that after immobilization or adsorption on TiO 2 surfaces [49] this soft blood protein undergoes conformational changes induced by the surface topography affecting also its tertiary structure, then the functionality [50][51][52][53][54][55][56][57][58]. These studies confirm the importance not only of the similar surface chemistry of TiO 2 surfaces but also the influence of the surface curvature on the protein-biomaterial interaction process.…”

Section: Introductionmentioning

confidence: 70%

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Raffaini

2021

Coatings

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TiO2 is widely used in biomaterial implants. The topography, chemical and structural properties of titania surfaces are an important aspect to study. The size of TiO2 nanoparticles synthetized by sol–gel method can influence the responses in the biological environment, and by using appropriate heat treatments different contents of different polymorphs can be formed. Protein adsorption is a crucial step for the biological responses, involving, in particular, albumin, the most abundant blood protein. In this theoretical work, using molecular mechanics and molecular dynamics methods, the adsorption process of an albumin subdomain is reported both onto specific different crystallographic faces of TiO2 anatase and also on its ideal three-dimensional nanosized crystal, using the simulation protocol proposed in my previous theoretical studies about the adsorption process on hydrophobic ordered graphene-like or hydrophilic amorphous polymeric surfaces. The different surface chemistry of anatase crystalline faces and the nanocrystal topography influence the adsorption process, in particular the interaction strength and protein fragment conformation, then its biological activity. This theoretical study can be a useful tool to better understand how the surface chemistry, crystal structure, size and topography play a key role in protein adsorption process onto anatase surface so widely used as biomaterial.

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Section: Introductionmentioning

confidence: 70%

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Raffaini

2021

Coatings

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“…The surface morphology, crystal structure, and surface hydroxyl groups of TiO 2 fibers can promote the adhesion and growth of MSCs on the material surface by affecting protein's adsorption and their conformational changes. 43 2.8. Osteogenic Differentiation of MSCs In Vitro.…”

Section: Migration Of Mscsmentioning

confidence: 99%

Aptamer/Hydroxyapatite-Functionalized Titanium Substrate Promotes Implant Osseointegration via Recruiting Mesenchymal Stem Cells

Wei

Chen

et al. 2022

ACS Appl. Mater. Interfaces

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Endowing bone regeneration materials with both stem cell recruitment and osteoinduction properties is a key factor in promoting osseointegration of titanium (Ti) implants. In this study, Apt19s-grafted oxidized hyaluronic acid (OHA) was deposited onto a protein-mediated biomineralization hydroxyapatite (HAp) coating of Ti. HAp was achieved by the treatment of lysozyme and tris(2-carboxyethyl) phosphonate mixture and then soaked in calcium ion (Ca2+) solution to obtain functional Ti substrate (Ti/HAp/OHA-Apt). In vitro studies confirmed that Ti/HAp/OHA-Apt could effectively maintain the sustained release of Apt19s from Ti for 7 days. The released Apt19s significantly enhanced the migration of bone marrow mesenchymal stem cells (MSCs), which was reflected by the experiment of transwell assay, wound healing, and zymogram detection. Compared with pure Ti, Ti/HAp/OHA-Apt was able to adjust the adsorption of functional proteins at the Ti-based interface to expose their active sites, which significantly increased the expression of adhesion-associated proteins (vinculin and tensin) in MSCs to promote their adhesion on Ti-based interface. In vitro cell experiments of alkaline phosphatase activity staining, mineralization detection, and expression of osteogenesis-related genes showed that Ti/HAp/OHA-Apt significantly enhanced the osteogenic differentiation ability of MSCs, which may be highly related to the porous structure of hydroxyapatite on Ti interface. In vivo test of Micro-CT, H&E staining, and histochemical staining further confirmed that Ti/HAp/OHA-Apt was able to promote MSC recruitment at the peri-implant interface to form new bone. This work provides a new approach to develop functional Ti-based materials for bone defect repair.

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“…The extent of changes in the secondary structure of BSA was not directly correlated with the WCA of the surfaces and BSA has undergone significant conformational changes even on the hydrophilic anodized surfaces. It is generally recognized that proteins undergo less denaturation upon adsorption on hydrophilic surfaces and the restriction of protein-surface interactions due to water-surface interactions on hydrophilic TiO2 surfaces were reported to cause less denaturation compared to hydrophobic surfaces (Zhao et al, 2020). However, this conclusion doesn't take the effect of surface morphology into consideration.…”

Section: Protein Conformationmentioning

confidence: 99%

“…Rockwell et al (2012), on the other hand, reported that increased surface curvature increases protein adsorption, and Lu et al (2012) reported that increasing surface roughness between 1-11 nm increases protein adsorption due to an increase in the surface free energy (Lu et al, 2012). Zhao et al (2020) reported that upon BSA adsorption on TiO 2 nanofibers, a significant increase in β-turn and β-sheet content was observed while the random coil structure disappeared. The diameters of these structures varied between 184 ± 8 nm and 511 ± 17 nm.…”

Section: Protein Conformationmentioning

confidence: 99%

“…The diameters of these structures varied between 184 ± 8 nm and 511 ± 17 nm. They interpreted that the dipole moment provided by the surface OH groups, which are strong enough to disrupt the H bonds in the protein structure, may have been responsible for the formation of a new secondary structure in the protein (Zhao et al, 2020). Yang et al (2021) investigated the adsorption of globular proteins on smooth and nano-rippled TiOx/Ti and SiOx/Si.…”

Section: Protein Conformationmentioning

confidence: 99%

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The Effect of Nanostructured Titanium Surface on Protein Adsorption

Şirin

Akdoğan

2022

Gazi University Journal of Science Part A: Engineering and Innovation

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The amount and conformation of bovine serum albumin upon adsorption on titanium (Ti) surfaces containing nanotubes with different pore sizes were investigated. Nanotubes were created on the surfaces via anodization. Protein adsorption behavior on anodized surfaces were compared with the adsorption behavior on smooth and sanded Ti surfaces. The conformational changes in surface adsorbed proteins were evaluated using the second derivative and curve fitting methods applied to the Fourier transform infrared spectra of the surfaces. Results showed that the amount of protein adsorbed on the surfaces increased significantly with increasing surface roughness and a significant change in the conformation of the adsorbed protein occurred on every surface albeit in a different fashion. When anodized samples were considered, it was observed that the changes in the secondary structure seemed to be correlated with to the pore size of the nanotubes rather than the surface roughness.

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Conformation changes of albumin and lysozyme on electrospun TiO2 nanofibers and its effects on MSC behaviors

Cited by 10 publications

References 37 publications

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Surface Chemistry, Crystal Structure, Size and Topography Role in the Albumin Adsorption Process on TiO2 Anatase Crystallographic Faces and Its 3D-Nanocrystal: A Molecular Dynamics Study

Aptamer/Hydroxyapatite-Functionalized Titanium Substrate Promotes Implant Osseointegration via Recruiting Mesenchymal Stem Cells

The Effect of Nanostructured Titanium Surface on Protein Adsorption

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