Confocal Raman Microscopy for Monitoring Chemical Reactions on Single Optically Trapped, Solid-Phase Support Particles

Houlne, Michael P.; Sjostrom, Christopher M.; Uibel, Rory H.; Kleimeyer, James A.; Harris, Joel M.

doi:10.1021/ac020325t

Cited by 89 publications

(89 citation statements)

References 51 publications

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“…Both Raman spectra and the size of the trapped droplet were used to monitor the uptake of p-nonylphenol into the toluene droplet and calculate the distribution coefficient of p-nonylphenol between both environments. Houlne et al used a silica microparticle trapped in solution far away from the sample chamber boundaries as a solid-phase support for model peptide synthesis reaction and monitored its kinetics without the disturbing influence of the reaction container surfaces on the free diffusion of the reagents into the reaction center [415]. One of the fastest developing and most successful applications of the RT is the study of individual living cells and artificial cell model systems.…”

Section: Raman Spectroscopy Of Optically Trapped Objects: Raman Tweezersmentioning

confidence: 99%

Light at work: The use of optical forces for particle manipulation, sorting, and analysis

2008

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We review the combinations of optical micro-manipulation with other techniques and their classical and emerging applications to non-contact optical separation and sorting of micro- and nanoparticle suspensions, compositional and structural analysis of specimens, and quantification of force interactions at the microscopic scale. The review aims at inspiring researchers, especially those working outside the optical micro-manipulation field, to find new and interesting applications of these methods.

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Section: Raman Spectroscopy Of Optically Trapped Objects: Raman Tweezersmentioning

confidence: 99%

Light at work: The use of optical forces for particle manipulation, sorting, and analysis

2008

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“…Through serial recording of the Raman spectra, the transformation of a biochemical reaction can thus be dynamically monitored. [15] By using confocal Raman spectroscopy, the chemical transformation of cellular bioactivity has been studied. [16,17] Single fission yeasts under different nutrients, stresses, and atmospheric conditions have also been examined to elucidate the role of a metabolic activity-related Raman band.…”

Section: Introductionmentioning

confidence: 99%

Real‐time molecular assessment on oxidative injury of single cells using Raman spectroscopy

Chang

Lin

Chen

et al. 2009

J Raman Spectroscopy

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Oxidative stress is encountered in many biological systems; the resultant oxidative injury plays a significant role in the pathogenesis of diverse diseases. Conventional measurements on oxidative injury are employed almost exclusively on a large population of cells either by counting the fraction of cell death or by observing the fluorometric change resulting from exogenous reagents, thereby lacking in molecular detail and temporal specificity. In this work we combine laser tweezers and Raman spectroscopy to observe the response of single cells to oxidative stress. By measuring the temporal changes of vibrational spectra of single optically trapped cells, we demonstrate a molecular-level assessment of cellular oxidative injury in real time, both qualitatively and quantitatively, without the introduction of exogenous reagents. The main experimental findings are supported by the observation of Raman spectra of intermediates and downstream products. The abrogation of the above changes by ascorbic acid further illustrates the therapeutic effect of antioxidants against cellular oxidative injury. This approach is extensible to studies exploring the biochemical transformation of single cells or intracellular organelles in response to various chemical or physical stimuli. With the aid of 'molecular fingerprints', single-cell Raman spectroscopy exhibits a great potential for accessing the chemical aspects of cellular bioactivity, yielding insight into pathophysiological processes and assisting the development of novel therapeutic interventions against diseases.

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“…Optical trapping on the other hand, being compatible with micro-or nanofluidic systems, is a well-established technique that has been widely applied to singlemolecule force and optical spectroscopy, non-invasive manipulation, and chemical reactions monitoring . [8][9][10][11][12][13] More recently, optical trapping has been applied to plasmonic noble metal nanoparticles. [14][15][16] Due to their localized surface plasmon resonances, noble metal nanoparticles provide strongly enhanced and highly localized electromagnetic fields close to their surface.…”

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confidence: 99%

Enhancing Single-Nanoparticle Surface-Chemistry by Plasmonic Overheating in an Optical Trap

Lutich

et al. 2012

Nano Lett.

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Surface-chemistry of individual, optically trapped plasmonic nanoparticles is modified and accelerated by plasmonic overheating. Depending on the optical trapping power, gold nanorods can exhibit red-shifts of their plasmon resonance (i.e. increasing aspect ratio) under oxidative conditions. In contrast, in bulk exclusively blue shifts (decreasing aspect ratios) are observed. Supported by calculations, we explain this finding by local temperatures in the trap exceeding the boiling point of the solvent which can not be achieved in bulk.Keywords noble metal nanoparticles; plasmon resonance; colloidal chemistry; nano-/microfluidics; gold nanorods; plasmonic heating Miniaturization is a successful approach to faster, more efficient applications in physics and chemistry. In chemistry, the reduction of reaction volumes is expected to lead to highthroughput, portable analytical and sensing applications, and fundamental insights into single-molecule chemical reactions. [1][2][3] Lab-on-a-chip applications frequently employ microor nanofluidic structures. [4][5][6][7] However, these approaches do not change the outcome of a chemical reaction. Optical trapping on the other hand, being compatible with micro-or nanofluidic systems, is a well-established technique that has been widely applied to singlemolecule force and optical spectroscopy, non-invasive manipulation, and chemical reactions monitoring . [8][9][10][11][12][13] More recently, optical trapping has been applied to plasmonic noble metal nanoparticles. [14][15][16] Due to their localized surface plasmon resonances, noble metal nanoparticles provide strongly enhanced and highly localized electromagnetic fields close to their surface. 17 Their optical and field-enhancing properties allow for manipulating and enhancing fluorescence, Raman scattering, charge and energy transfer, and local temperature. [18][19][20][21] The surface plasmon resonances can be tuned through controlling size, shape and material of the nanoparticles via advanced colloidal chemistry. 22 Gold nanorods for instance, display two different plasmon modes: one transversal, in which the electrons oscillate perpendicular to the long axis of the rod, and the other red shifted longitudinal, in which the electrons oscillate along the long axis of the nanorod. 23 Europe PMC Funders Author Manuscripts sensitively depends on the nanorod's aspect ratio: the higher the aspect ratio, the more red shifted the longitudinal plasmon resonance.Here we apply the plasmonic heating effect of a single gold nanoparticle in an optical trap to both accelerate and modify chemical reactions on the surface of the particle. We show that individual optically trapped plasmonic particles modify and accelerate surface-chemistry by plasmonic overheating to yield different and faster results than the corresponding bulk reactions. Individual gold nanorods under oxidative conditions can display a red-shift of their localized surface plasmon resonances corresponding to an increasing aspect ratio, while in bulk exclusively bl...

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Confocal Raman Microscopy for Monitoring Chemical Reactions on Single Optically Trapped, Solid-Phase Support Particles

Cited by 89 publications

References 51 publications

Light at work: The use of optical forces for particle manipulation, sorting, and analysis

Light at work: The use of optical forces for particle manipulation, sorting, and analysis

Real‐time molecular assessment on oxidative injury of single cells using Raman spectroscopy

Enhancing Single-Nanoparticle Surface-Chemistry by Plasmonic Overheating in an Optical Trap

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