Confocal Raman mapping of collagen cross-link and crystallinity of human dentin–enamel junction

Slimani, Amel; Nouioua, Fares; Desoutter, Alban; Levallois, Bernard; Cuisinier, Frédéric; Tassery, Hervé; Terrer, Elodie; Salehi, Hamideh

doi:10.1117/1.jbo.22.8.086003

Cited by 24 publications

(34 citation statements)

References 28 publications

(32 reference statements)

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“…The main advantage of Raman microscopy in this study was to obtain the chemical composition at a microscopic scale and the orientation of the crystals using polarized light simultaneously. Composition and structure study of sound or carious enamel were already published by our group . Confocal polarized Raman spectroscopy allows to assess the c‐axis orientation variation following modified intensity of some Raman peaks (OH 3572 cm −1 , ν 1 PO 4 3− 960 cm −1 , ν 2 PO 4 3− 432 cm −1 , and ν 3 PO 4 3− 1028 cm −1 ).…”

Section: Introductionmentioning

confidence: 99%

Cross striation in human permanent and deciduous enamel measured with confocal Raman microscopy

Desoutter

Slimani

Al-Obaidi

et al. 2019

J Raman Spectroscopy

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Dental enamel is the most mineralized tissue in the human body and has a very complex organization. The aim of the present study was to evaluate chemical composition and orientation of crystals in human enamel cross striation using confocal Raman microscope. Slices of teeth were prepared and scanned using a confocal Raman microscope. Cross striation spacing was calculated according to the variation of PO43− peak intensity. The measurements showed a characteristic length between two cross striations of 2.70 (SD 0.43) μm for permanent teeth and 1.75 (SD 0.37) for deciduous teeth. Ratios between OH and PO43− peaks, for 0 and 90 degrees angulation, are, respectively, 9.18 (SD 0.80) and 44.74 (SD 3.12) for decidual teeth, and 11.72 (SD 1.46) and 22.12 (SD 3.15) for adult teeth. This confirmed that the hydroxyapatite crystal is well oriented along the enamel prism, as reported in the literature, but with a significant increase in deciduous teeth. K‐mean cluster binary images were calculated to compare the ratio of the centroid peaks of the cross striation zone and inter cross striation material. The regions corresponding to inner prism, included cross striations, are more mineralized than the outer part of enamel prism. In the prism, the Pearson correlation test was performed based on the intensity of hydroxyapatite characteristic peaks (ν1 PO43−, ν1 CO32−, and OH). The variations were well correlated: These results indicated that cross striations corresponded to the variation of hydroxyapatite concentration and not to a variation of crystals orientation.

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Section: Introductionmentioning

confidence: 99%

Cross striation in human permanent and deciduous enamel measured with confocal Raman microscopy

Desoutter

Slimani

Al-Obaidi

et al. 2019

J Raman Spectroscopy

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“…Crystallinity degree in enamel is determined by measuring changes in peaks ratio of symmetric mode of phosphate at 960 cm −1 over 950 cm −1 20 .…”

Section: Methodsmentioning

confidence: 99%

Chemical & Nano-mechanical Study of Artificial Human Enamel Subsurface Lesions

Al-Obaidi

Salehi

Desoutter

et al. 2018

Sci Rep

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White lesions represent an early phase of caries formation. 20 human sound premolars were subjected to pH cycling procedure to induce subsurface lesions (SLs) in vitro. In addition, 2 teeth with naturally developed white spot lesions (WSLs) were used as references. All specimens characterized by confocal Raman microscopy being used for the first time in examining white & subsurface lesions and providing a high resolution chemical and morphological map based on phosphate peak intensity alterations at 960 cm−1. Nanoindentation technique was used to measure Hardness (H) and Young’s modulus (E) of enamel. Phosphate map of examined samples exhibited presence of intact surface layer (ISL) followed by severe depletion in (PO43−) peak in the area corresponding to the body of the lesion. In all examined groups, the mechanical properties of enamel were decreased in lesion area and found to be inversely related to penetration depth of indenter owing to enamel hierarchical structure. By combining the above two techniques, we linked mechanical properties of enamel to its chemical composition and ensured that the two methods are highly sensitive to detect small changes in enamel composition. Further work is required to bring these two excellent tools to clinical application to perceive carious lesions at an early stage of development.

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“…Dentin crystallinity refers to the degree of mineral crystal in hydroxyapatite phase within the dentin structure [20]. It is related to calcium phosphate crystals deposition inside or between collagen fibers [21]. Both mineral and organic composition have an impact on the mechanical properties of dental structure [20].…”

Section: Discussionmentioning

confidence: 99%

Long-Term Impact of New Calcium-Silicate-Based Sealer on Mineral Contents and Crystallinity of Radicular Dentin: An Ex Vivo Study

Zeid

Saif

Alsofi

et al. 2020

Journal of Spectroscopy

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Introduction. This study aimed to evaluate the long-term impact of new calcium silicate-based sealer (HiFlow Bioceramic) compared to calcium hydroxide-based sealer (Sealapex) on mineral constituents and crystallinity of radicular dentin. Methods. Roots of multirooted maxillary molars were denuded their dentin from all covered cementum then longitudinally split. The dentin segments were divided into three groups covered with either HiFlow root canal sealer or Sealapex root canal sealer or kept untreated (control group). After complete setting of sealers, the radicular dentin of each group was stored in phosphate buffer solution or deionized water for 60 days and then examined with Fourier Infrared Spectroscopy, scanning electron microscope/energy dispersed X-ray and X-ray diffraction (XRD). Results. When the dentin was stored in phosphate buffer solution, compared with untreated dentin, Fourier transform infrared spectra showed insignificant increase in the amide I and phosphate, significant increase in carbonate area, and carbonate/phosphate ratio, while there was insignificant change in phosphate/amide I ratio of HiFlow-treated dentin, whereas Sealapex induced significant decrease in amide I area, phosphate area, and phosphate/amide ratio. Both sealers induced significant increase in crystallinity index and significant decrease in crystallinity percent. Energy dispersed X-ray showed decrease in calcium content by both sealers. The phosphate content was increased by HiFlow and decreased by Sealapex. Conclusions. The mineral composition and crystallinity of dentin were insignificantly changed by the long-term HiFlow treatment, whereas, markedly changes by Sealapex.

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Confocal Raman mapping of collagen cross-link and crystallinity of human dentin–enamel junction

Cited by 24 publications

References 28 publications

Cross striation in human permanent and deciduous enamel measured with confocal Raman microscopy

Cross striation in human permanent and deciduous enamel measured with confocal Raman microscopy

Chemical & Nano-mechanical Study of Artificial Human Enamel Subsurface Lesions

Long-Term Impact of New Calcium-Silicate-Based Sealer on Mineral Contents and Crystallinity of Radicular Dentin: An Ex Vivo Study

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