Confocal Micro-Raman Spectroscopy: Theory and Application to a Hybrid Polymer Coating

Baia, Lucian; Gigant, K.; Posset, Uwe; Schottner, G.; Kiefer, W.; Popp, Jürgen

doi:10.1366/0003702021955006

Cited by 59 publications

(44 citation statements)

References 16 publications

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“…Due to the coherent nature of the CARS process, the resultant signal strength is boosted compared to linear confocal Raman microscopy and, hence, CARS microscopy enables fast image acquisition (Potma et al, 2001). However, in contrast to conventional Raman microspectroscopy (Baia et al, 2002;Rö sch et al, 2003;Harz et al, 2005), single-mode CARS microscopy yields images based on univariant results. Thereby, the information content to be obtained is limited to the spatial distribution of a single preselected Raman-active mode.…”

mentioning

confidence: 99%

Toward in Vivo Chemical Imaging of Epicuticular Waxes

Weissflog

Vogler²,

Akimov³

et al. 2010

Plant Physiology

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Epicuticular waxes, which are found on the outer surface of plant cuticles, are difficult to study in vivo. To monitor the growth, development, and structural alterations of epicuticular wax layers, coherent anti-Stokes Raman scattering (CARS) might be used. CARS, as a Raman-based technique, not only provides structural insight but also chemical information by imaging the spatial distribution of Raman-active vibrations. Here, we present a comparative study using CARS and scanning electron microscopy to characterize the structure of epicuticular waxes. The ability of CARS to provide detailed structural information on the biologically important wax layer was detailed on the examples of cherry laurel (Prunus laurocerasus), hoya (Hoya carnosa), and ceriman/Swiss cheese plant (Monstera sp. aff. deliciosa). We anticipate that the work presented will open a doorway for online monitoring of formation and alterations of epicuticular wax layers.

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confidence: 99%

Toward in Vivo Chemical Imaging of Epicuticular Waxes

Weissflog

Vogler²,

Akimov³

et al. 2010

Plant Physiology

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“…As Raman spectroscopy relies on the inelastic scattering of light, detection of the Raman signal in back-scattering geometry is possible, which is beneficial for in-vivo clinical applications. Though Raman microspectroscopy, in particular in confocal geometry, provides a high chemical specificity with good spatial resolution in the order of below 500 nm [25,26], i.e. roughly half the wavelength when using near-infrared lasers for excitation.…”

Section: Biophotonicsmentioning

confidence: 99%

Multimodal imaging to study the morphochemistry of basal cell carcinoma

Vogler

Meyer

Akimov

et al. 2010

Journal of Biophotonics

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Basal cell carcinoma is the most abundant malignant neoplasm in humans, the pathology of which is characterized by an abnormal proliferation of basal cells. Basal cell carcinoma can show a variety of different morphologies, which are based on different cellular biology. Furthermore, the carcinoma often grows invisibly to the eye imbedded in the surrounding skin. Therefore, in some cases its clinical detection is challenging. Thus, our work aims at establishing an unsupervised tissue classification method based on multimodal imaging and the application of chemometrics to discriminate basal cell carcinoma from non‐diseased tissue. A case study applying multimodal imaging to ex‐vivo sections of basal cell carcinoma is presented. In doing so, we apply a combination of various linear and non‐linear imaging modalities, i.e. fluorescence, Raman and second‐harmonic generation microscopy, to study the morphochemistry of basal cell carcinoma. The joint information content obtained by such multimodal approach in studying various aspects of the malignant tissue alterations associated with basal cell carcinoma is discussed. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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“…45 This, however, does not take into account the diffraction and the effect of the confocal aperture, which has been calculated by various authors to different levels. 41,42,46,47 The work of Sourisseau and Maraval, 48 generally thought to be the most complete model, reduces the compression to ×1.7. In general, it has usually been found to be between ×1.5 and ×2.…”

Section: Depth Resolutionmentioning

confidence: 99%

The Role of ConfocalRaman Spectroscopy in Food Science

Pudney

Hancewicz

2001

Handbook of Vibrational Spectroscopy

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This chapter outlines why confocal Raman has developed into a tool that can offer unique insights into food science. It shows how the inherent strengths of Raman spectroscopy of chemical specificity and structural sensitivity can be exploited on the micron length scale. It covers the essentials elements of how a confocal Raman microscope system works. This is followed by a practical description of the way in which a confocal Raman system should be used and also how to avoid some of the common pitfalls, especially with regard to depth resolution. Raman spectra can contain a mass of information and, in order to fully use this, considerable effort needs to put into data analysis. Appropriate methodologies are discussed in some detail with discussion on the strategies one can employ, with enlightening examples to show practically the results that can be obtained.

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Confocal Micro-Raman Spectroscopy: Theory and Application to a Hybrid Polymer Coating

Cited by 59 publications

References 16 publications

Toward in Vivo Chemical Imaging of Epicuticular Waxes

Toward in Vivo Chemical Imaging of Epicuticular Waxes

Multimodal imaging to study the morphochemistry of basal cell carcinoma

The Role of ConfocalRaman Spectroscopy in Food Science

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