Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in mouse skin: does S. aureus generally produce a biofilm on damaged skin?

Akiyama, Hisanori; Huh, Wook Kang; Yamada, Osamu; Oono, Takashi; Iwatsuki, Keiji

doi:10.1046/j.1365-2133.2002.04962.x

Cited by 63 publications

(46 citation statements)

References 20 publications

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“…These studies firmly establish that biofilm-like structures are present in the murine wound infection model. [14][15][16][17][18] More recently, Parsek and Singh 19 have proposed criteria to help translate biofilm research into the clinical arena. The criteria they established are as follows: (a) The infecting bacteria are adherent to some substratum or are surface associated, (b) Direct examination of infected tissue shows bacteria living in cell clusters, or microcolonies, encased in an ECM.…”

Section: Discussionmentioning

confidence: 99%

Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo

Davis

Ricotti

Cazzaniga

et al. 2007

Wound Repair Regeneration

322

252

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A biofilm is a collection of microbial cells that are attached to a surface and embedded in a self-produced extrapolymeric substance. The understanding of the biofilm phenotype is important in the understanding of bacteria in vitro but it has been difficult to translate biofilm science to the clinical setting. More recently, preliminary criteria for defining biofilm associated diseases have been proposed and the purpose of this study was to create a biofilm-associated wound model based on these criteria. Using a porcine model, partial thickness wounds were inoculated with a wound isolate Staphylococcus aureus strain. Wounds were then treated with either one of two topical antimicrobial agents (mupriocin cream or triple antibiotic ointment) within 15 minutes to represent planktonic bacteria or 48 hours after initial inoculation to represent biofilm-associated wound infection. Using light microscopy, scanning electron microscopy and epifluorescence microscopy, we were able to observe biofilm-like structures in wounds after 48 hours of inoculation and occlusion. The in vivo antimicrobial assay was used to demonstrate that both mupirocin cream and the triple antibiotic ointment were effective in reducing planktonic S. aureus but had reduced efficacy against biofilm-embedded S. aureus. Our results demonstrated that S. aureus form firmly attached microcolonies and colonies of bacteria encased in an extracellular matrix on the surface of the wounds. These biofilm-like communities also demonstrated increased antimicrobial resistance when compared with their planktonic phenotype in vivo. The structural and physiological results support the hypothesis that bacterial biofilms play a role in wound colonization and infection.

show abstract

Section: Discussionmentioning

confidence: 99%

Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo

Davis

Ricotti

Cazzaniga

et al. 2007

Wound Repair Regeneration

322

252

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show abstract

“…It has been observed previously that S. aureus can produce a biofilm on damaged rat skin tissue, which plays a crucial role in colonization and adherence to dermal epithelium. 14 In addition, biofilm-forming bacteria are more resistant to antibiotics and are even resistant to immunological attack. 14,15 Our findings have shown that damage to the intact skin following CO 2 laser application might partially be compensated by the significant reduction in the levels of microbial flora.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Effect of Carbon Dioxide Laser-Skin Resurfacing and Mechanical Abrasion on the Skin's Microbial Flora in an Animal Model

Manolis

Tsakris

Kaklamanos

et al. 2006

Dermatologic Surgery

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“…Since S. aureus can generally produce a biofilm on damaged skin tissue, antimicrobial agents may not eradicate S. aureus without the help of neutrophils. The S. aureus glycocalyx may play a crucial role in colonization and adherence to damaged skin tissue [40].…”

Section: Biofilm Formationmentioning

confidence: 99%

Staphylococcal cutaneous infections: Invasion, evasion and aggression

Iwatsuki

Morizane

Oono

2006

Journal of Dermatological Science

148

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Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in mouse skin: does S. aureus generally produce a biofilm on damaged skin?

Abstract: As S. aureus cells can generally produce a biofilm on damaged skin tissues, antimicrobial agents may not eradicate S. aureus cells without the help of PMNs. S. aureus glycocalyx may play a crucial role in colonization and adherence to damaged skin tissues.

Cited by 63 publications

References 20 publications

Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo

Microscopic and physiologic evidence for biofilm‐associated wound colonization in vivo

In Vivo Effect of Carbon Dioxide Laser-Skin Resurfacing and Mechanical Abrasion on the Skin's Microbial Flora in an Animal Model

Staphylococcal cutaneous infections: Invasion, evasion and aggression

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