Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding

Rukke, Bjørn Arne; Cholidis, Symira; Johnsen, Arild; Ottesen, Preben

doi:10.2478/s11686-014-0242-2

Cited by 9 publications

(4 citation statements)

References 21 publications

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“…Many researchers reported Hypoderma tarandi, the reindeer warble fly, as the causative agent (Kearney et al 1991;Lagace-Wiens et al 2008). Larval DNA barcoding was used to confirm H. tarandi as the causative agent in one of the studies (Rukke et al 2014). Cases of ophthalmomyiasis due to C. bezziana, the screwworm fly had been reported from Malaysia (Alhady et al 2008) and from India (Nene et al 2015).…”

Section: Ophthalmomyiasismentioning

confidence: 99%

Incidence of myiasis among humans—a review

Singh

2015

Parasitol Res

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Myiasis, the infestation of tissues of live humans and other vertebrate animals with dipteran larvae (maggots), is a phenomenon of widespread occurrence throughout the tropical regions of the world. It is commonly seen in domestic and wild animals but occurs rarely in man also. The tissue invasion in man by maggots is generally a well-recognized complication of neglected wounds. The condition may be asymptomatic but occasionally results in more or less severe problems and even death when larvae invade body cavities or areas that forbid their direct visual examination. Many cases of myiasis, however, usually do not reach the attention of medical practitioners because of the tendency of the mature larvae to migrate out of the lesion for pupation. In the present study, the authors reviewed the past literature describing the cases of human myiasis, causative fly species and predisposing factors for the onset of the same. The aim of the present manuscript is to provide a database for the future reference of medical entomologists, medical practitioners and veterinarians. Review of literature revealed that 37 fly species belonging to ten families were responsible worldwide for the onset of human myiasis. The incidence of myiasis among humans can be correlated to increasing fly populations, poor hygienic conditions and presence of domestic animals in the close vicinity. Other factors responsible for myiasis include neglected open wounds and foul-smelling discharge from natural body openings. Moreover, ignorance plays a key role in its occurrence since people are generally unaware about the actual cause and often correlate the condition to their superstitious beliefs. It is emphasized that the patients should not neglect their wounds and should take proper medical care; otherwise, myiasis may supervene.

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Section: Ophthalmomyiasismentioning

confidence: 99%

Incidence of myiasis among humans—a review

Singh

2015

Parasitol Res

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“…However, these traditional methods of identification pose problems in case of closely related species. In this context, DNA barcoding is a practical tool for species identification, when morphological classification of an organism is difficult (Bjorn et al, 2014). Species-level characterisation can explain more about its pathogenicity, host specificity, life cycle and so on.…”

Section: Discussionmentioning

confidence: 99%

Classical morphology and DNA barcoding based identification of freshwater ectoparasite, Argulus foliaceus in rohu Labeo rohita

Mohanty¹,

Mohanty²,

Sarma

et al. 2023

Indian J. Fish.

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DNA barcoding coupled with classical morphological description have become promising approach for the species levelidentification. In the present study, 278 numbers of rohu Labeo rohita were screened, for Argulus infestation, amongwhich 167 fish (60.07%) were found infested with Argulus. Morphological attributes of the parasites were studied by lightmicroscope (LM) and Scanning Electron Microscope (SEM) which showed dorsoventrally flattened body comprising ofhead, thorax, abdomen, a pair of large compound eyes, suctorial organs with sclerotised support structures and two openingsof spermatheca at posterior end. Posterior incision of abdomen do not reach the mid-line and the cephalo-thoracic carapacehave not extended beyond the beginning of abdomen. Further validation was done using molecular tools for accurateparasite identification. The pair-wise genetic distance value, using Kimura-2 parameter showed a species level variationof 0.001 (1%) with that of A. foliaceus, while 0.083 and 0.052 (i.e. more than 2%) with that of A. indicus and A. japonicusrespectively. Phylogenetic tree generated using Neighbour-Joining (NJ) and Maximum-Likelihood (ML) methods, withKimura-2 parameter were also in agreement with pairwise distance values. The mitochondrial cytochrome c oxidase subunit1 (COI) sequences of A. foliaceus formed one cluster with the present studied samples, whereas sequences of A. japonicusformed a sister group. Integrating morphological and rapid DNA barcoding tools, the species was delineated as A. foliaceus. Keywords: Argulus foliaceus, COI gene, DNA barcoding, Labeo rohita, Morphology

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“…Morfologické vlastnosti larvy Oestrus ovis jsou poměrně typické (Obrázky 8 a 9), ale mohou se lišit a je doporučováno molekulární ověřovací testování [12]. To však bylo jen zřídka aplikováno v humánní medicíně [13]. Z hlediska veřejného zdraví, je také důležité vzhledem k rychlé diagnostice humánních a veterinárních případů myiasis mimo endemické oblasti, aby se zabránilo rozvoji mouchy v nových regionech, kde se dříve nevyskytovaly [7].…”

Section: Diskuseunclassified

External Ophthalmomyiasis Caused by Oestrus Ovis (A Case Report)

Hartmannová

Mach

Záruba

et al. 2020

CSO

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The work deals with atypical conjunctival infection of Czech patient with Oestrus ovis larvae. Ophthalmomyiasis is infestation of mammalian eyes by the larvae or worms of some flies. The most common cause of human myiasis is the Sheep. Shepherds are infected in habitats, but human eye disease outside the areas of abundant hamsters is rare. We describe a case of eye disease in a middle-aged man from the Czech Republic who spent a summer holiday seven weeks before examination in the north of Greece. During the first examination he was completely treated and no further problems were reported. Ophthalmomyiasis externa should be considered as a possible infection of travelers to the southern endemic regions when returning with an acute causeless onset of a one-sided foreign body sensation in the eye.

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Confirming Hypoderma tarandi (Diptera: Oestridae) human ophthalmomyiasis by larval DNA barcoding

Cited by 9 publications

References 21 publications

Incidence of myiasis among humans—a review

Incidence of myiasis among humans—a review

Classical morphology and DNA barcoding based identification of freshwater ectoparasite, Argulus foliaceus in rohu Labeo rohita

External Ophthalmomyiasis Caused by Oestrus Ovis (A Case Report)

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