“…In addition, AO collection requires general anaesthesia in adults. Larger volumes of CSF are readily obtained from the AO site in comparison with the LS site, although large volumes are rarely required (except when performing myelography), but removing a large volume can lead to brain herniation and death in animals with increased intracranial pressure (Lorenz and Kornegay 2004). As a result, AO taps are usually contraindicated when the clinician considers that intracranial pressure may be raised (e.g.…”