Confirmation of multiple tetracycline residues in milk and oxytetracycline in shrimp by liquid chromatography–particle beam mass spectrometry

Carson, Mary C; Ngoh, Maureen A.; Hadley, Stephen W

doi:10.1016/s0378-4347(98)00171-6

Cited by 59 publications

(33 citation statements)

References 14 publications

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“…Nine closely related compounds-tetracycline, rolitetracycline, oxytetracycline, chlortetracycline, demeclocycline, meclocycline, methacycline, doxycycline, and minocycline-are widely used in human and veterinary medicine. The tetracyclines have been monitored by high pressure liquid chromatography (HPLC) with ultraviolet or fluorescence detection [29], thin layer chromatography and LC/FAB/MS [30,31], LC/particle beam/MS [32], and ESI/MS [33][34][35][36][37][38][39][40]. Mass spectrometric techniques for the analysis of tetracycline antibiotics in foods have been reviewed [41].…”

mentioning

confidence: 99%

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Kamel

Fouda

Brown

et al. 2002

J. Am. Soc. Mass Spectrom.

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Electrospray ionization (ESI) and collisionally induced dissociation (CID) mass spectra were obtained for five tetracyclines and the corresponding compounds in which the labile hydrogens were replaced by deuterium by either gas phase or liquid phase exchange. The number of labile hydrogens, x, could easily be determined from a comparison of ESI spectra obtained with N 2 and with ND 3 as the nebulizer gas. CID mass spectra were obtained forϪ , and produced by ESI using a Sciex API-III plus and a Finnigan LCQ ion trap mass spectrometer. Compositions of product ions and mechanisms of decomposition were determined by comparison of the MS N spectra of the un-deuterated and deuterated species. Protonated tetracyclines dissociate initially by loss of H 2 O (D 2 O) and NH 3 (ND 3 ) if there is a tertiary OH at C-6. The loss of H 2 O (D 2 O) is the lower energy process. Tetracyclines without the tertiary OH at C-6 lose only NH 3 (ND 3 ) initially. MS N experiments showed easily understandable losses of HDO, HN(CH 3 ) 2 , CH 3 Ϫ N¢CH 2 , and CO from fragment ions. The major fragment ions do not come from cleavage reactions of the species protonated at the most basic site. Deprotonated tetracyclines had similar CID spectra, with less fragmentation than those observed for the protonated tetracyclines. The lowest energy decomposition paths for the deprotonated tetracyclines are the competitive loss of NH 3 (ND 3 ) or HNCO (DNCO). Product ions appear to be formed by charge remote decompositions of species de-protonated at the C-10 phenol. (J Am Soc Mass Spectrom 2002, 13, 543-557)

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confidence: 99%

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Kamel

Fouda

Brown

et al. 2002

J. Am. Soc. Mass Spectrom.

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“…Therefore, the easy availability and efficiency for the treatment of bacterial infected disease, they were widely used in animal husbandry and aquaculture [5][6][7] . As with other veterinary antibiotics, when tetracycline is used in food animals, it has the potential to generate drug residues in the animals and animal products which can lead to increases in microbial resistance.…”

Section: Introductionmentioning

confidence: 99%

LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples

REDDY¹,

Sudhakar²,

Rao³

et al. 2017

Orient. J. Chem

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A modest liquid chromatography/tandem mass spectrometric method was developed and validated to determine three tetracycline antibiotics and their epimers tetracycline, oxytetracycline, chlortetracycline, 4-epi tetracycline, 4-epi oxytetracycline, 4-epi chlortetracycline in shrimp samples respectively. The procedure was involved the extraction of tetracyclines and its epimers from homogenized sample with EDTA-McIlvaine buffer followed by solid phase extraction clean up with Oasis HLB cartridge. The analysis was carried out using Inertsil ODS-3V, 5 micron, 150×4.6 mm column and mobile phase consists of 5 mM Oxalic acid in water (A) and 0.1% formic acid in methanol (B) in gradient mode at a flow rate of 0.8 mL/minutes. The final extracts were analyzed by the sensitive and selective LC/ESI/MS/MS operating in positive ion multiple reaction monitoring (MRM) mode.

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“…These compounds are commonly used in veterinary medicine to prevent diseases and as an additive in animal foods to promote growth. [1][2][3] Tetracyclines are widely used in diary cattle, poultry and shrimp. 3,4 To ensure food safety to consumers, EU, FAO/WHO and FDA have set safe levels for residue tetracyclines in milk (0.1 mg/kg), 3 liver (0.3 mg/kg), egg (0.2 mg/kg), and muscle tissue (0.1 mg/kg).…”

Section: Introductionmentioning

confidence: 99%

“…Several analytical methods have been reported for the determination of tetracylines in various samples, such as milk, [1][2][3][6][7][8][9] shrimp, 1,4 animal feeds, 5,10 animal tissues [11][12][13] and pharmaceutical formulations 14,15 based on thin-layer chromatography, 10 capillary electrophoresis, 16,17 and high performance liquid chromatography (HPLC). [6][7][8][11][12][13][14] HPLC is a common method that separates tetracyclines in the reversephase mode with different detection methods, such as spectrophotometry, 12,13 fluorometry, 18,19 mass spectrometry, 20,21 and electrochemistry.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection

et al. 2005

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Confirmation of multiple tetracycline residues in milk and oxytetracycline in shrimp by liquid chromatography–particle beam mass spectrometry

Cited by 59 publications

References 14 publications

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

Mass spectral characterization of tetracyclines by electrospray ionization, H/D exchange, and multiple stage mass spectrometry

LC-MS/MS Method, Development and Validation to Determine Three Tetracyclines and Their Epimers in Shrimp Samples

Analysis of Tetracycline Antibiotics Using HPLC with Pulsed Amperometric Detection

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