Confirmation of cross-pollination of Ardisia crenata by sequence-characterized amplified region (SCAR) markers

Roh, Mark S.; Lee, Ae-Kyung; Suh, Jeung Keun; Slovin, Janet P.

doi:10.1016/j.scienta.2006.05.013

Cited by 12 publications

(10 citation statements)

References 10 publications

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“…Ardisia crenata Sims (Myrsinaceae) is a small evergreen shrub native in a range from Japan to India occurring in the understory of tropical and subtropical forests (Bailey 1925;Roh et al 2006). The species is insect-pollinated and self-compatible like many congeners (Cheon et al 2000;Pascarella 1997;Zhao et al 2006).…”

Section: Study Speciesmentioning

confidence: 99%

Species diversity and population density affect genetic structure and gene dispersal in a subtropical understory shrub

Zeng

Michalski

Fischer

et al. 2012

Journal of Plant Ecology

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Aims The dispersal of pollen and seeds is spatially restricted and may vary among plant populations because of varying biotic interactions, population histories or abiotic conditions. Because gene dispersal is spatially restricted, it will eventually result in the development of spatial genetic structure (SGS), which in turn can allow insights into gene dispersal processes. Here, we assessed the effect of habitat characteristics like population density and community structure on small-scale SGS and estimate historical gene dispersal at different spatial scales. Methods In a set of 12 populations of the subtropical understory shrub Ardisia crenata, we assessed genetic variation at 7 microsatellite loci within and among populations. We investigated small-scale genetic structure with spatial genetic autocorrelation statistics and heterogeneity tests and estimated gene dispersal distances based on population differentiation and on within-population SGS. SGS was related to habitat characteristics by multiple regression. Important Findings The populations showed high genetic diversity (H e = 0.64) within populations and rather strong genetic differentiation (F # ST = 0.208) among populations, following an isolation-by-distance pattern, which suggests that populations are in gene flow-drift equilibrium. Significant SGS was present within populations (mean Sp = 0.027). Population density and species diversity had a joint effect on SGS with low population density and high species diversity leading to stronger small-scale SGS. Estimates of historical gene dispersal from between-population differentiation and from within-population SGS resulted in similar values between 4.8 and 22.9 m. The results indicate that local-ranged pollen dispersal and inefficient long-distance seed dispersal, both affected by population density and species diversity, contributed to the genetic population structure of the species. We suggest that SGS in shrubs is more similar to that of herbs than to trees and that in communities with high species diversity gene flow is more restricted than at low species diversity. This may represent a process that retards the development of a positive species diversitygenetic diversity relationship. Keywords: Ardisia crenata d BEF China d gene flow d habitat effects d spatial genetic structure

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Section: Study Speciesmentioning

confidence: 99%

Species diversity and population density affect genetic structure and gene dispersal in a subtropical understory shrub

Zeng

Michalski

Fischer

et al. 2012

Journal of Plant Ecology

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“…Cultivated populations usually have a complex demographic and genetic history due to artificial hybridization and multiple sites and timing of introduction-this is common among ornamental plants such as Hypericum perforatum, Pueria lobata,a n dSchinus terebinthifolius (Maron et al 2004;Sun et al 2005;Williams et al 2005). Roh et al (2006) did find that a cultivar of A. crenata was hybridized from other cultivars. Indeed, we observed two genetic clusters for the invaded individuals, indicating there were at least two genetic sources in the invasive region.…”

Section: Discussionmentioning

confidence: 99%

Inferring the invasion history of coral berry Ardisia crenata from China to the USA using molecular markers

Niu

Wang

et al. 2012

Ecological Research

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Genetic comparisons between native and invasive populations of a species can provide insights into its invasion history information, which is useful for guiding management and control strategies. The coral berry Ardisia crenata was introduced to Florida last century as a cultivated ornament plant, and has since spread widely throughout the southern regions of the USA. Previously, the genetic variation among 20 natural populations of A. crenata across its distribution center in southern China was quantified using seven microsatellite markers. Here we expand on that work by additionally sampling individuals from four other native populations in Taiwan and Japan, and from five invasive populations in the USA. We also examined the results from one chloroplast intergenic spacer region (trnF‐trnL) in all 29 populations. Our aim is to identify the invasion source and subsequent history of the species’ spread throughout the southern USA. We observed lower genetic diversity in the invasive populations based on both microsatellite and chloroplast markers. Our data show that the invasive populations can be clustered with native populations in southeastern China, inferring this region as the geographic origin of A. crenata cultivars invading the USA. We further classified invasive individuals into invasive I and invasive II clusters. Nantou in Taiwan and Xihu in mainland China are the most closely related populations to those, which identify the former as potential sources for host‐specific control agents. Our results, combined with the known introduction records, suggest that A. crenata was first multiply introduced into Florida and then secondarily colonized Louisiana and Texas from Florida.

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“…Clearly, when using RAPD markers in progeny analysis, it is easier to reject an apomictic origin (the second approach) based on the presence of paternal specific markers in progeny as that is a clear and absolute indication of their hybrid origin. Several studies have adopted this approach (Shoyamaa et al 1998;Elisario et al 1999;Obata et al 2000;Lo et al 2002;Divakaran et al 2006;and Roh et al 2006).…”

Section: Discussionmentioning

confidence: 99%

No evidence of apomixis in matroclinal progeny from experimental crosses in the genus Fragaria (strawberry) based on RAPDs

2009

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Crossing experiments in Fragaria have frequently resulted in some matroclinal progeny. These have been attributed to either accidental selfing and/or pollen contamination, or apomixis. In this study 202 carefully controlled intra and interspecific crosses in Fragaria were made. No progeny were produced from 164 interploid crosses made between diploids, hexaploids and octoploids but 38 intraploid crosses resulted in 904 F 1 plants, of which 42 (4.6%) were found to be morphologically matroclinal. All matroclinal progeny subsequently studied by RAPDs were found to be hybrids and not apomicts. The absence of apomixis among matroclinal progeny in this study casts doubt on previous reports of apomixis in Fragaria. Our results showed that the complete morphological similarity of progeny to the seed parent, even in recessive characteristics, cannot therefore be taken as evidence of apomixis, and may rather indicate the heterozygosity of the pollen parent, emphasising the need to use DNA markers for confirmation of apomixis.

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Confirmation of cross-pollination of Ardisia crenata by sequence-characterized amplified region (SCAR) markers

Cited by 12 publications

References 10 publications

Species diversity and population density affect genetic structure and gene dispersal in a subtropical understory shrub

Species diversity and population density affect genetic structure and gene dispersal in a subtropical understory shrub

Inferring the invasion history of coral berry Ardisia crenata from China to the USA using molecular markers

No evidence of apomixis in matroclinal progeny from experimental crosses in the genus Fragaria (strawberry) based on RAPDs

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