A greenhouse study was conducted to determine the Cu-efficiency of 5A/5RL wheat-rye translocation lines and nine wheat genotypes grown in a Cu-deficient orthic dark grey chernozemic soil. The soil used was characterized by pH 5.4 and 0.48 #g Cu g-l soil (DTPA), and was obtained from a known Cu-deficient site at Stony Plain, Central Alberta. Twelve genotypes (three 5AJ5RL wheat-rye translocation lines and nine wheat cultivars) and two Cu treatments (soil amended with 145 #g Cu kg -l soil, and non-amended) were used. The efficiency for Cu use was evaluated for grain yield and yield components. 5A/5RL wheat-rye translocation lines individually and as a group showed significantly (p<0.05) higher grain yield both in +Cu and -Cu treatments compared to wheat genotypes without the wheat-rye chromosome translocation. 5AJ5RL wheat-rye translocation lines demonstrated Cu-efficiency ranging from 70-127% for grain yield. Only two wheat cultivars (Kenya Leopard and Columbus) showed comparable efficiency (70 and 76% respectively). Wheat cultivars Kwale, Kenya Tausi, Roblin, Katepwa, Park, Oslo and Biggar showed low Cu-efficiency (10-36%). The study confirms that the 5AJ5RL wheat-rye translocation confers useful levels of Cu-efficiency to wheat, that can be used as an additional source of variability in breeding programs.