Conductive Gel Increases the Small Tumor Treatment With Electrochemotherapy Using Needle Electrodes

Suzuki, Daniela Ota Hisayasu; Marques, Claudia M.G.; Rangel, Marcelo Monte Mór

doi:10.1111/aor.12631

Cited by 20 publications

(18 citation statements)

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“…E,F and E,F). This conductivity value agrees with the gel conductivity proposed by Ivorra et al and Suzuki et al . We suggest a ¼ dilution of 0.9% sodium chloride and bleomycin (1500 UI).…”

Section: Discussionsupporting

confidence: 92%

Novel application for electrochemotherapy: Immersion of nasal cavity in dog

et al. 2016

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Electrochemotherapy is a new modality of local cancer treatment that increases the delivery of chemotherapy drugs into tumor cells by applying intense electric fields. This novel electrochemotherapy application was applied as an adjuvant to surgery and eliminated intranasal tumors in dog. The treatment challenges are the surgery limitations due to anatomy and residual tumor in the bone cavity. Most of the tumoral mass on nasal cavity was surgically removed. The internal nasal cavity was immersed in liquid and bleomycin before applying electric field. The solution was necessary to increase the superficial contact between plate electrodes and residual tumor. The numerical study demonstrated electrochemotherapy efficiency in different clinical situations. The proximity between electrodes and bone (<3 mm) and bone irregularities affect the electric field distribution on tumoral tissue. The tumoral tissue around bone protuberances tends to be eliminated. Electrochemotherapy with plate electrodes inside the cavity might not be effective. Different values of electric conductivity solution were studied; the ideal value was 0.5 S/m. The numerical and experimental results confirm the successful application of electrochemotherapy on dog nasal cavity.

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Section: Discussionsupporting

confidence: 92%

Novel application for electrochemotherapy: Immersion of nasal cavity in dog

et al. 2016

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“…New electroporation protocols are constantly being developed since electroporation is very sensitive to the homogeneity of the treated sample—e.g., the conductive gel in the electrode-tissue interface or the medium viscosity can alter treatment efficacy significantly (Ivorra et al, 2008; Sungailaite et al, 2015; Suzuki, Marques & Rangel, 2016). The non-homogeneous sample conductivity could be addressed by applying, high-frequency bipolar pulse protocols (H-FIRE) which mitigate the impedance differences (Bhonsle et al, 2015; Sano et al, 2015) but unfortunately require delivery of higher energy than longer unipolar irreversible electroporation (IRE) pulses (Murovec et al, 2016; Sweeney et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Novickij

Dermol

Grainys

et al. 2017

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BackgroundCell membrane permeabilization by pulsed electromagnetic fields (PEMF) is a novel contactless method which results in effects similar to conventional electroporation. The non-invasiveness of the methodology, independence from the biological object homogeneity and electrical conductance introduce high flexibility and potential applicability of the PEMF in biomedicine, food processing, and biotechnology. The inferior effectiveness of the PEMF permeabilization compared to standard electroporation and the lack of clear description of the induced transmembrane transport are currently of major concern.MethodsThe PEMF permeabilization experiments have been performed using a 5.5 T, 1.2 J pulse generator with a multilayer inductor as an applicator. We investigated the feasibility to increase membrane permeability of Chinese Hamster Ovary (CHO) cells using short microsecond (15 µs) pulse bursts (100 or 200 pulses) at low frequency (1 Hz) and high dB/dt (>106 T/s). The effectiveness of the treatment was evaluated by fluorescence microscopy and flow cytometry using two different fluorescent dyes: propidium iodide (PI) and YO-PRO®-1 (YP). The results were compared to conventional electroporation (single pulse, 1.2 kV/cm, 100 µs), i.e., positive control.ResultsThe proposed PEMF protocols (both for 100 and 200 pulses) resulted in increased number of permeable cells (70 ± 11% for PI and 67 ± 9% for YP). Both cell permeabilization assays also showed a significant (8 ± 2% for PI and 35 ± 14% for YP) increase in fluorescence intensity indicating membrane permeabilization. The survival was not affected.DiscussionThe obtained results demonstrate the potential of PEMF as a contactless treatment for achieving reversible permeabilization of biological cells. Similar to electroporation, the PEMF permeabilization efficacy is influenced by pulse parameters in a dose-dependent manner.

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“…The treatment effectiveness depends on the capacity of the system to produce a local electric field high enough to open pores around the entire tumor [ 8 – 10 , 12 ]. In the simulation environment, the local electric field indicates whether the electroporation of the tumor cells is theoretically viable.…”

Section: Methodsmentioning

confidence: 99%

Electrochemotherapy Effectiveness Loss due to Electric Field Indentation between Needle Electrodes: A Numerical Study

Berkenbrock

Machado

Suzuki

2018

Journal of Healthcare Engineering

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Electrochemotherapy is an anticancer treatment based on applying electric field pulses that reduce cell membrane selectivity, allowing chemotherapy drugs to enter the cells. In parallel to electrochemotherapy clinical tests, in silico experiments have helped scientists and clinicians to understand the electric field distribution through anatomically complex regions of the body. In particular, these in silico experiments allow clinicians to predict problems that may arise in treatment effectiveness. The current work presents a metastatic case of a mast cell tumor in a dog. In this specific treatment planning study, we show that using needle electrodes has a possible pitfall. The macroscopic consequence of the electroporation was assessed through a mathematical model of tissue electrical conductivity. Considering the electrical and geometrical characteristics of the case under study, we modeled an ellipsoidal tumor. Initial simulations were based on the European Standard Operating Procedures for electrochemotherapy suggestions, and then different electrodes' arrangements were evaluated. To avoid blind spots, multiple applications are usually required for large tumors, demanding electrode repositioning. An effective treatment electroporates all the tumor cells. Partially and slightly overlapping the areas increases the session's duration but also likely increases the treatment's effectiveness. It is worth noting that for a single application, the needles should not be placed close to the tumor's borders because effectiveness is highly likely to be lost.

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Conductive Gel Increases the Small Tumor Treatment With Electrochemotherapy Using Needle Electrodes

Cited by 20 publications

References 25 publications

Novel application for electrochemotherapy: Immersion of nasal cavity in dog

Novel application for electrochemotherapy: Immersion of nasal cavity in dog

Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses

Electrochemotherapy Effectiveness Loss due to Electric Field Indentation between Needle Electrodes: A Numerical Study

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