Novel application for electrochemotherapy: Immersion of nasal cavity in dog

Abstract

Electrochemotherapy is a new modality of local cancer treatment that increases the delivery of chemotherapy drugs into tumor cells by applying intense electric fields. This novel electrochemotherapy application was applied as an adjuvant to surgery and eliminated intranasal tumors in dog. The treatment challenges are the surgery limitations due to anatomy and residual tumor in the bone cavity. Most of the tumoral mass on nasal cavity was surgically removed. The internal nasal cavity was immersed in liquid and bleomycin before applying electric field. The solution was necessary to increase the superficial contact between plate electrodes and residual tumor. The numerical study demonstrated electrochemotherapy efficiency in different clinical situations. The proximity between electrodes and bone (<3 mm) and bone irregularities affect the electric field distribution on tumoral tissue. The tumoral tissue around bone protuberances tends to be eliminated. Electrochemotherapy with plate electrodes inside the cavity might not be effective. Different values of electric conductivity solution were studied; the ideal value was 0.5 S/m. The numerical and experimental results confirm the successful application of electrochemotherapy on dog nasal cavity.

Minimally invasive electrochemotherapy procedure for treating nasal duct tumors in dogs using a single needle electrode

“…This procedure is very complex and prone to fail because the misplacement of the needles leaves areas of the tumor untreated. In some cases, open surgery is needed for placing the needles correctly 16 , such as in the case reported by Suzuki et al 17 , where the results were very good though the procedure required open surgery and the filling of the nasal cavity with gel to improve conductivity. These was not needed in our case since the electrode is inserted through the nasal orifice and, secretions and blood present in the nasal duct replaced conductive gel.…”

Electroporation as the Immunotherapy Strategy for Cancer in Veterinary Medicine: State of the Art in Latin America

“…In particular, the group of Dr. Marshall made several publications on this topic [ 10 , 70 , 97 , 102 , 103 , 104 , 105 , 106 , 107 , 108 ]. The use of ECT in veterinary medicine began in 2008 in Brazil by the group of Dr. Rangel [ 109 , 110 , 111 , 112 , 113 , 114 ], closely followed by the group of Dr. Marshall, from Argentina, in 2009. Although the treatment had been used in human medicine in Europe since 2006, there were no devices available in Latin America for human nor veterinary medicine at that time.…”

A Influência Do Nitrogênio Em Aços Inoxidáveis Austeníticos Com Aplicação Em Próteses Ortopédicas

“…A economia de recursos também é possível em casos que o experimento prático envolve destruição mecânica da amostra, necessita de infraestrutura com grandes dimensões e/ou características não encontradas facilmente na natureza (e.g., condições de pressão e temperatura), ou a própria amostra é de alto custo (e.g., desenvolvimento de embarcações). Em áreas de experimentação biológica, o uso de simulações pode reduzir ou substituir a quantidade de animais necessários para um determinado estudo, pois resultados preliminares já podem ser obtidos no ambiente de simulação (JONES; GARDNER; WATSON, 2009); como também, em casos que não há possibilidade de ensaio prático, a simulação pode informar a solução com maior chance de sucesso (SUZUKI et al, 2015(SUZUKI et al, , 2017.…”