Conductive chitosan/polyaniline hydrogel with cell-imprinted topography as a potential substrate for neural priming of adipose derived stem cells

Eftekhari, Behnaz Sadat; Eskandari, Mahnaz; Janmey, Paul A.; Samadikuchaksaraei, Alí; Gholipourmalekabadi, Mazaher

doi:10.1039/d1ra00413a

Cited by 18 publications

(20 citation statements)

References 76 publications

(90 reference statements)

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“…In consideration of the latter, the molecular mechanisms that have largely been proposed to affect nerve healing appear to do so primarily through enhanced survival, proliferation and neuronal differentiation, described in several reports in the literature. Recently, Eftekhari et al (169) demonstrated the capability of a novel conductive chitosan/polyaniline hydrogel in modulating neuronal differentiation through the upregulation of the MAP2 gene in rat adipose-derived stem cells at the material-tissue interface. Notably, conductivity and cell-imprinted topography were investigated simultaneously as biophysical cues to enhance the potential for neural regeneration.…”

Section: Molecular Mechanisms For Improved Regenerative Responses Of ...mentioning

confidence: 99%

“…Recently, Eftekhari et al ( 169 ) demonstrated the capability of a novel conductive chitosan/polyaniline hydrogel in modulating neuronal differentiation through the upregulation of the MAP2 gene in rat adipose-derived stem cells at the material-tissue interface. Notably, conductivity and cell-imprinted topography were investigated simultaneously as biophysical cues to enhance the potential for neural regeneration.…”

Section: Challenges For Nsc Transplantation In Scimentioning

confidence: 99%

“…Similarly, Moroder et al ( 171 ) analyzed morphological changes in PC-12 cells and reported significant increases in the percentage of neurite bearing cells, neurites per cell and neurite lengths of PC-12 cells in the presence of ES compared with no ES on conductive polymer scaffolds. Notably, these studies employed cell lines, and recent preclinical developments appear to heavily report on cell lines for SCI repair applications ( 169 , 172 , 173 ). Whilst cell lines have their advantages in mitigating ethical implications arising from ESCs or fetal-derived NSCs, the move to physiologically relevant NSCs in CNS injury preclinical models would seem to be concomitant with clinical translation for SCI.…”

Section: Challenges For Nsc Transplantation In Scimentioning

confidence: 99%

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Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury

Mutepfa

Hardy

Adams

2022

Front. Med. Technol.

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Spinal cord injury (SCI) is a serious condition caused by damage to the spinal cord through trauma or disease, often with permanent debilitating effects. Globally, the prevalence of SCI is estimated between 40 to 80 cases per million people per year. Patients with SCI can experience devastating health and socioeconomic consequences from paralysis, which is a loss of motor, sensory and autonomic nerve function below the level of the injury that often accompanies SCI. SCI carries a high mortality and increased risk of premature death due to secondary complications. The health, social and economic consequences of SCI are significant, and therefore elucidation of the complex molecular processes that occur in SCI and development of novel effective treatments is critical. Despite advances in medicine for the SCI patient such as surgery and anaesthesiology, imaging, rehabilitation and drug discovery, there have been no definitive findings toward complete functional neurologic recovery. However, the advent of neural stem cell therapy and the engineering of functionalized biomaterials to facilitate cell transplantation and promote regeneration of damaged spinal cord tissue presents a potential avenue to advance SCI research. This review will explore this emerging field and identify new lines of research.

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Section: Molecular Mechanisms For Improved Regenerative Responses Of ...mentioning

confidence: 99%

Section: Challenges For Nsc Transplantation In Scimentioning

confidence: 99%

Section: Challenges For Nsc Transplantation In Scimentioning

confidence: 99%

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Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury

Mutepfa

Hardy

Adams

2022

Front. Med. Technol.

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“…Electric fields can be better regulated and applied than other stimulation sources. Electric field-responsive hydrogels are mainly composed of polyelectrolyte materials (polythiophene, polypyrrole, and polyaniline), which can convert electrical and mechanical energy under electric field stimulation [ 53 , 54 , 55 ]. The macroporous structure of the hydrogel is more likely to cause volume collapse than the microporous structure in the electric field [ 56 ].…”

Section: Classification Of Smart Hydrogelsmentioning

confidence: 99%

Recent Advances in Smart Hydrogels Prepared by Ionizing Radiation Technology for Biomedical Applications

et al. 2022

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Materials with excellent biocompatibility and targeting can be widely used in the biomedical field. Hydrogels are an excellent biomedical material, which are similar to living tissue and cannot affect the metabolic process of living organisms. Moreover, the three-dimensional network structure of hydrogel is conducive to the storage and slow release of drugs. Compared to the traditional hydrogel preparation technologies, ionizing radiation technology has high efficiency, is green, and has environmental protection. This technology can easily adjust mechanical properties, swelling, and so on. This review provides a classification of hydrogels and different preparation methods and highlights the advantages of ionizing radiation technology in smart hydrogels used for biomedical applications.

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“…Giving perspective to this notion, we, herein, blended PAni nanofibers (PNFs) with a deacetylated derivative of natural polysaccharide chitin, i.e., chitosan (Ch). Ch is an FDA approved natural biopolymer, which is biocompatible and biodegradable, and therefore, it has been widely explored for a range of biomedical applications including TE. , In spite of the synergy of their beneficial effects, fabrication and characterization of composites of PAni and Ch have been reported by only a handful of research groups in different configurations, viz., thin film, hydrogel, and electrospun mesh for TE applications. − The major reason for such limited studies is the lack of proper cell binding sites, for which PNF:Ch based nanocomposites were coated with costly biomolecules such as laminin for PC12 attachment …”

Section: Introductionmentioning

confidence: 99%

Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity

Borah

Das

Upadhyay³

2022

ACS Appl. Bio Mater.

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Bioscaffolds having electrically conducting polymers (CPs) have become increasingly relevant in tissue engineering (TE) because of their ability to regulate conductivity and promote biological function. With this in mind, the current study shows a conducting polyaniline nanofibers (PNFs) dispersed chitosan (Ch) nanocomposites scaffold with a simple one-step surface functionalization approach using glutaraldehyde for potential neural regeneration applications. According to the findings, 4 wt % PNFs dispersion in Ch matrix is an optimal concentration for achieving desirable biological functions while maintaining required physicochemical properties as evidenced by SEM, XRD, current–voltage (I–V) measurement, mechanical strength test, and in vitro biodegradability test. Surface chemical compositional analysis using XPS and ATR FT-IR confirms the incorporation of aldehyde functionality after functionalization, which is corroborated by surface energy calculations following the Van Oss–Chaudhury–Good method. Surface functionalization induced enhancement in surface hydrophilicity in terms of the polar component of surface energy (γ i AB) from 6.35 to 12.54 mN m–1 along with an increase in surface polarity from 13.61 to 22.54%. Functionalized PNF:Ch scaffolds demonstrated improvement in enzyme activity from 67 to 94% and better enzyme kinetics with a reduction of Michaelis constants (K m) from 21.55 to 13.81 mM, indicating favorable protein–biomaterial interactions and establishing them as biologically perceptible materials. Surface functionalization mediated improved cell–biomaterial interactions led to improved viability, adhesion, and spreading of primary adipose derived mesenchymal stem cells (ADMSCs) as well as improved immunocompatibility. Cytoskeletal architecture assessment under differentiating media containing 10 ng/mL of each basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) revealed significant actin remodeling with neurite-like projections on the functionalized scaffolds after 14 days. Immunocytochemistry results showed that more than 85% of cells expressed early neuron specific β III tubulin protein on the functionalized scaffolds, whereas glial fibrillary acidic protein (GFAP) expression was limited to approximately 40% of cells. The findings point to the functionalized nanocomposites’ potential as a smart scaffold for electrically stimulated neural regeneration, as they are flexible enough to be designed into microchanneled or conduit-like structures that mimic the microstructures and mechanical properties of peripheral nerves.

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Conductive chitosan/polyaniline hydrogel with cell-imprinted topography as a potential substrate for neural priming of adipose derived stem cells

Abstract: A conductive chitosan/polyaniline hydrogel with cell-imprinted topography was used for neural priming of adipose derived stem cells.

Cited by 18 publications

References 76 publications

Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury

Electroactive Scaffolds to Improve Neural Stem Cell Therapy for Spinal Cord Injury

Recent Advances in Smart Hydrogels Prepared by Ionizing Radiation Technology for Biomedical Applications

Surface Functionalized Polyaniline Nanofibers:Chitosan Nanocomposite for Promoting Neuronal-like Differentiation of Primary Adipose Derived Mesenchymal Stem Cells and Urease Activity

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