Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

Bober, Patrycja; Humpolíček, Petr; Pachernı́k, Jiřı́; Stejskal, Jaroslav; Lindfors, Tom

doi:10.1039/c5ra07504a

Cited by 30 publications

(22 citation statements)

References 64 publications

(84 reference statements)

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“…However, utilizing PANI in biosensing and tissue engineering assumes that substantial knowledge exists on its bio-interface properties. In this context, a number of studies have already been published, e.g., PANI films doped with perchloride, hydrochloride, malic and citric acid are suitable for adhesion and proliferation of PC-12 [ 7 ], PANI films deposited with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid (PAMPSA) allow an embryonic stem cell to adhere and grow [ 8 ]. Therefore, polyaniline coating has been used in the past to improve the physical and biological properties of materials such as polyurethane [ 9 ] or graphene and graphene oxide [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Rejmontová

Capáková

Mikušová

et al. 2016

IJMS

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Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is.

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Section: Introductionmentioning

confidence: 99%

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Rejmontová

Capáková

Mikušová

et al. 2016

IJMS

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“…In contrast to aniline-based copolymers, which are only highly conductive at pH lower than 2 (the main problem to be solved for their application in cell interfaces), PEDOT was stable at physiological pH values of 7.4. 48 , 58 , 68 See the Supporting Information (Figure S3).…”

Section: Resultsmentioning

confidence: 99%

“… 52 − 56 However, polyaniline (PANI)-based polymers still show cytotoxicity because of their conductive emeraldine forms being stable only at pH levels lower than 4, thereby limiting their use in biomedical applications. 48 , 57 , 58 In this sense, other CPs, such as those based on poly(3,4-ethylenedioxythiphene) (PEDOT), arise as an alternative for biomedical applications because they are electroactive in the whole pH range.…”

Section: Introductionmentioning

confidence: 99%

Novel Conducting and Biodegradable Copolymers with Noncytotoxic Properties toward Embryonic Stem Cells

et al. 2018

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Electroactive biomaterials that are easily processed as scaffolds with good biocompatibility for tissue regeneration are difficult to design. Herein, the synthesis and characterization of a variety of novel electroactive, biodegradable biomaterials based on poly(3,4-ethylenedioxythiphene) copolymerized with poly(d,l lactic acid) (PEDOT-co-PDLLA) are presented. These copolymers were obtained using (2,3-dihydrothieno[3,4-b][1,4]dioxin-2-yl)methanol (EDOT-OH) as an initiator in a lactide ring-opening polymerization reaction, resulting in EDOT–PDLLA macromonomer. Conducting PEDOT-co-PDLLA copolymers (in three different proportions) were achieved by chemical copolymerization with 3,4-ethylenedioxythiophene (EDOT) monomers and persulfate oxidant. The PEDOT-co-PDLLA copolymers were structurally characterized by 1H NMR and Fourier transform infrared spectroscopy. Cyclic voltammetry confirmed the electroactive character of the materials, and conductivity measurements were performed via electrochemical impedance spectroscopy. In vitro biodegradability was evaluated using proteinase K over 35 days, showing 29–46% (w/w) biodegradation. Noncytotoxicity was assessed by adhesion, migration, and proliferation assays using embryonic stem cells (E14.tg2a); excellent neuronal differentiation was observed. These novel electroactive and biodegradable PEDOT-co-PDLLA copolymers present surface chemistry and charge density properties that make them potentially useful as scaffold materials in different fields of applications, especially for neuronal tissue engineering.

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“…Our results indicate that the cells continued to proliferate over a period of 72 h in the presence of scaffolds. Although cell proliferation continued over time, the rate of cell growth for the PANI bound construct was lower compared to the controls due to the known cytotoxicity of PANI [ 80 ]. This is most likely that proliferation continued due to the synergistic effects of the protein components of the scaffolds, which play a role in enhancing biocompatibility of the PANI bound scaffold.…”

Section: Resultsmentioning

confidence: 99%

Development of Self-Assembled Nanoribbon Bound Peptide-Polyaniline Composite Scaffolds and Their Interactions with Neural Cortical Cells

2018

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Degenerative neurological disorders and traumatic brain injuries cause significant damage to quality of life and often impact survival. As a result, novel treatments are necessary that can allow for the regeneration of neural tissue. In this work, a new biomimetic scaffold was designed with potential for applications in neural tissue regeneration. To develop the scaffold, we first prepared a new bolaamphiphile that was capable of undergoing self-assembly into nanoribbons at pH 7. Those nanoribbons were then utilized as templates for conjugation with specific proteins known to play a critical role in neural tissue growth. The template (Ile-TMG-Ile) was prepared by conjugating tetramethyleneglutaric acid with isoleucine and the ability of the bolaamphiphile to self-assemble was probed at a pH range of 4 through 9. The nanoribbons formed under neutral conditions were then functionalized step-wise with the basement membrane protein laminin, the neurotropic factor artemin and Type IV collagen. The conductive polymer polyaniline (PANI) was then incorporated through electrostatic and π–π stacking interactions to the scaffold to impart electrical properties. Distinct morphology changes were observed upon conjugation with each layer, which was also accompanied by an increase in Young’s Modulus as well as surface roughness. The Young’s Modulus of the dried PANI-bound biocomposite scaffolds was found to be 5.5 GPa, indicating the mechanical strength of the scaffold. Thermal phase changes studied indicated broad endothermic peaks upon incorporation of the proteins which were diminished upon binding with PANI. The scaffolds also exhibited in vitro biodegradable behavior over a period of three weeks. Furthermore, we observed cell proliferation and short neurite outgrowths in the presence of rat neural cortical cells, confirming that the scaffolds may be applicable in neural tissue regeneration. The electrochemical properties of the scaffolds were also studied by generating I-V curves by conducting cyclic voltammetry. Thus, we have developed a new biomimetic composite scaffold that may have potential applications in neural tissue regeneration.

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Conducting polyaniline based cell culture substrate for embryonic stem cells and embryoid bodies

Abstract: Noncytotoxic polyaniline–poly(2-acrylamido-2-methyl-1-propanesulfonate) films which are electrically conducting at the physiological pH were applied as cell culture substrate. The films demonstrate selective interaction with specific target cells.

Cited by 30 publications

References 64 publications

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Novel Conducting and Biodegradable Copolymers with Noncytotoxic Properties toward Embryonic Stem Cells

Development of Self-Assembled Nanoribbon Bound Peptide-Polyaniline Composite Scaffolds and Their Interactions with Neural Cortical Cells

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