Conditions Limiting Multiplication of Fibroblastic and Epithelial Cells in Dense Cultures

Dulbecco, Renato; Elkington, Jane

doi:10.1038/246197a0

Cited by 135 publications

(46 citation statements)

References 11 publications

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“…However, CFE of tumour cells in the lungs was not affected by anticoagulant treatment with mucous heparin, and was greatly stimulated by CS. a synthetic heparin which is a powerful anticoagulant and also increases fibrinolysis (Rothschild, 1968 (Stoker, 1973;Dulbecco and Elkington, 1973). It is suggested that the capacity of different types of tumours to produce GSS varies widely and may be a quality closely related to that of malignant (autonomous) behaviour of a tumour and may also affect the chances of survival and clonogenicity of seeded tumour cells.…”

Section: Subcutaneous Growth Of Tumour In Inflaned Pawmentioning

confidence: 99%

Promotion of Growth of Tumour Cells in Acutely Inflamed Tissues

Brenk¹,

Stone²,

Kelly³

et al. 1974

Br J Cancer

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Section: Subcutaneous Growth Of Tumour In Inflaned Pawmentioning

confidence: 99%

Promotion of Growth of Tumour Cells in Acutely Inflamed Tissues

Brenk¹,

Stone²,

Kelly³

et al. 1974

Br J Cancer

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“…These may be some locally acting growth inhibitors produced by the cells or local changes of pH etc. These alterations may decrease the ability of cells to utilize components of the medium, especially serum (Dulbecco and Elkington, 1973 (Vasiliev and Gelfand, 1973) so that a large part of the surface of each neoplastic cell is at a considerable distance from underlying normal cells. This may decrease the effectiveness of the inhibitory action of normal cells in mixed cultures.…”

mentioning

confidence: 99%

Neoplastic fibroblasts sensitive to the growth inhibition by homologous cells but insensitive to inhibition by parent normal cells

Pletyushkina¹,

Vasiliev²,

Gelfand³

1975

Br J Cancer

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Summary-3H-thymidine labelling and autoradiography were used to compare density dependent inhibition of growth in the cultures of two transformed lines of hamster fibroblasts and in primary cultures of their parent normal cells. Similar manifestations of density dependent inhibition were found in the isolated cultures of normal and neoplastic cells: at saturation densities these cultures had low labelling indices; these indices considerably increased when the cells migrated into the wound from the dense sheet, prelabelled cells seeded on the dense sheets of unlabelled homologous cells did not proliferate. However, proliferation of neoplastic cells was not inhibited when they were seeded on the dense sheet of normal fibroblasts. Thus, neoplastic hamster fibroblasts of both lines retained sensitivity to the inhibiting effect of homologous neoplastic cells but completely lost sensitivity to the inhibiting effect of normal fibroblasts. The possible significance of this selective loss of the sensitivity to normal cells is discussed briefly.INSENSITIVITY to the local inhibiting effect of homologous cells (loss ofthe homologous density dependent inhibition of growth) is usually regarded as one of the characteristic traits of transformed fibroblasts. At the same time, certain lines of transformed cells retain sensitivity to heterologous inhibition: their growth is inhibited by normal fibroblasts (Stoker, 1964;Ponten and MacIntyre, 1968;Weiss and Njeuma, 1971 The cells were grown in small (10 ml)

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“…Thus, the demonstration that FDGF stimulates the uptake of nucleosides, glucose, and 86Rb+ (Figs. 6 and 7), as well as the preliminary observation that it induces a fall in cyclic AMP, strongly implicates each of these early events as an integral part of the proliferative response.…”

mentioning

confidence: 99%

“…Many mammalian cells stop growing when the culture medium becomes depleted of growth factors. Such cells, resting in the GI/Go or A (1) state, are stimulated to synthesize DNA and proliferate when fresh serum is supplied (2)(3)(4)(5)(6)(7). Although the serum-dependent proliferative response has been intensively investigated as an in vitro model of growth regulation, its biochemical basis remains obscure.…”

mentioning

confidence: 99%

An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Bourne¹

1976

Proc. Natl. Acad. Sci. U.S.A.

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Prior to the onset of DNA synthesis, serum induces an array of "early events" (8-16) that do not require de novo protein synthesis. This array includes stimulation of the uptake of nucleosides (8-10), potassium (11), phosphate (8-10), and nutrients such as glucose (12, 13) as well as changes in cellular content of cyclic nucleotides (10,14). These early events are potentially important both as links, in the biochemical chain of events, leading to proliferation, and as markers for investigating the interactions of proliferative stimuli with their cellular receptors (15,16 In the final step not previously described, active fractions were pooled, neutralized, dialyzed against 5 mM sodium phosphate at pH 7.5, and applied to a 1 X 25 cm carboxymethylcellulose column (Whatman CM-52) previously equilibrated with the same buffer. After washing with 25 ml of column buffer, activity was eluted with a 140 ml linear gradient of 060 mM NaCl. In most preparations two peaks of activity were seen' the second and larger peak (eluting at 200-300 mM

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Conditions Limiting Multiplication of Fibroblastic and Epithelial Cells in Dense Cultures

Cited by 135 publications

References 11 publications

Promotion of Growth of Tumour Cells in Acutely Inflamed Tissues

Promotion of Growth of Tumour Cells in Acutely Inflamed Tissues

Neoplastic fibroblasts sensitive to the growth inhibition by homologous cells but insensitive to inhibition by parent normal cells

An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

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