An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Bourne, Henry R.

doi:10.1073/pnas.73.12.4555

Cited by 83 publications

(30 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results demonstrate that retinoids markedly enhance the mitogenic potency of a range of growth factors including TPA. Since high concentrations of FDGF [14] or the combination of insulin, EGF, and vasopressin [l 11 added in the absence of retinoids produce nearly maximal stimulation of DNA synthesis in Swiss 3T3 cells, retinoids are not required as essential nutrient molecules in this system. Their effects are probably due t o a hormone-like, regulatory modulation of the mitogenic response.…”

Section: Retinoids and Tpamentioning

confidence: 99%

Synergistic stimulation of early events and DNA synthesis by phorbol esters, polypeptide growth factors, and retinoids in cultured fibroblasts

Dicker¹

1979

J Supramol Struct

View full text Add to dashboard Cite

12-O-Tetradecanoyl-phorbol-13-acetate (TPA), in the absence of serum, acts synergistically with a range of polypeptide growth factors to stimulate DNA synthesis in quiescent Swiss 3T3 cells. These growth factors include epidermal growth factor (EGF), insulin, and the peptide produced by BHK cells transformed by SV-40 virus (fibroblast-derived growth factor, FDGF). Retinoids also show mitogenic synergism with TPA or polypeptide growth factors. The spectrum of mitogenic synergisms displayed by TPA are similar to those of vasopressin, a pituitary peptide. However, TPA and vasopressin do not synergistically interact to stimulate DNA synthesis in quiescent 3T3 cells. This suggests that TPA and vasopressin act via an identical biochemical pathway. Several lines of evidence suggest rapid postreceptor convergence of the mitogenic mechanisms of action of the hormone and the tumor promotor. Thus, vasopressin and TPA both inhibit EGF binding to cellular receptors. Furthermore, TPA and vasopressin induce a similar array of early events in quiescent cells--most strikingly, identical stimulation of Rb+ influx. Stimulation of ion flux is suggested as the possible convergence point of the pathway by which TPA and vasopressin act as mitogens.

show abstract

Section: Retinoids and Tpamentioning

confidence: 99%

Synergistic stimulation of early events and DNA synthesis by phorbol esters, polypeptide growth factors, and retinoids in cultured fibroblasts

Dicker¹

1979

J Supramol Struct

View full text Add to dashboard Cite

show abstract

“…Alternatively, it is possible that the effect of somatomedin deficiency in serum is masked by the IAbbreviations used in this paper: BSA, bovine serum albumin; DME, Dulbecco's modified minimum essential somatomedin production of the cultured cells themselves. Indeed, there are reports of production and release of growth factors into medium of cultured transformed cells (13)(14)(15) and cells that proliferate in very low serum concentrations (16). Explants of fetal mouse and rat liver (17,18) and a number of other fetal mouse tissues are able to secrete immunoreactive somatomedin into the culture medium.…”

mentioning

confidence: 99%

Hormonal Control of Immunoreactive Somatomedin Production by Cultured Human Fibroblasts

Clemmons¹,

Underwood²,

Wyk³

1981

J. Clin. Invest.

331

127

View full text Add to dashboard Cite

A B S T R A C T Human growth hormone (hGH) is known to be a potent stimulator of somatomedin secretion in vivo. The induction of somatomedin by growth hormone has been difficult to study in vitro, however, because no organ containing a high concentration of sQmatomedin has been identified. Because fetal mouse explants have been shown to produce somatomedin in vitro, we have undertaken studies to determine whether postnatal human fibroblast monolayers also produce somatomedin, and if so, whether its production is regulated by other hormones. Quiescent human fibroblasts were exposed to serumfree minimum essential medium, and the medium was assayed for somatomedin concentration using a specific radioimmunoassay for somatomedin-C. A progressive rise in immunoreactive somatomedin to 0.08 U/ml per 105 cells per 24 h was observed over 72 h of incubation. This was an underestimation of the actual concentration of immunoreactive somatomedin since the amount measured following acid treatment was at least fourfold higher than in the untreated medium. Growth hormone stimulated immunoreactive somatomedin production in a dose-dependent manner: 5 ng hGH/ml = 0.1 U/ml per 105 cells; 50 ng hGH/ml = 0.25 U/ml per 105 cells. Platelet-derived growth factor and fibroblast growth factor were also stimulatory, but epidermal growth factor, thyroxine, or cortisol had no effect. Media that had been exposed to human fibroblasts stimulated DNA synthesis in BALB/c 3T3 fibroblasts (a cell type that does not produce somatomedin). Medium-derived immunoreactive somatomedin eluted from Sephacryl S-200 in two major peaks (150,000 and 8,000 mol wt).

show abstract

“…Many oncogenically transformed cells in culture produce peptide growth factors (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). Typically, these growth factors have been assayed by their ability to induce a round of cell division or tritiated thymidine uptake in density-inhibited or serum-restricted heterologous cells.…”

mentioning

confidence: 99%

Transforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system.

Kaplan¹,

Anderson²,

Ozanne³

1982

Proc. Natl. Acad. Sci. U.S.A.

186

View full text Add to dashboard Cite

Kirsten murine sarcoma virus (KiMSV)-transformed rat-i, normal rat kidney (NRK), and BALB/c 3T3 cells are capable of continual growth in a serum-free medium supplemented with transferrin and insulin but with no exogenous mitogenic growth factors. Cells transformed by a mutant ofKiMSV that is temperature sensitive for the maintenance of transformation grow in this medium at the permissive temperature only. At the nonpermissive temperature, growth is dependent upon the presence of serum-free conditioned medium from the transformed cells. Normal rat-I cells are also dependent upon factors from the transformed cells for growth in this serum-free/mitogen-free medium. The serum-derived growth factors, epidermal growth factor, and fibroblast growth factor have no effecton the transformed cells, although epidermal growth factor can replace transforming growth factors produced by KiMSV-transformed cells for the growth of rat-i cells. Growth of the transformed cells in serumfree medium at clonal densities is dependent upon the presence ofconditioned medium collected from the same cells grown to high densities. These results show that (i) growth in serum-free/mitogen-free medium is a general property of KiMSV-transformed cells and (ii) growittof the transformed cells in this medium is dependent upon the presence of growth factors known to be produced by the cells, and theyprovide support for the hypothesis that serum-free growth ofKiMSV-transformed cells is dependent upon ectopically produced growth factors.

show abstract

An 18,000 molecular weight polypeptide induces early events and stimulates DNA synthesis in cultured cells.

Cited by 83 publications

References 28 publications

Synergistic stimulation of early events and DNA synthesis by phorbol esters, polypeptide growth factors, and retinoids in cultured fibroblasts

Synergistic stimulation of early events and DNA synthesis by phorbol esters, polypeptide growth factors, and retinoids in cultured fibroblasts

Hormonal Control of Immunoreactive Somatomedin Production by Cultured Human Fibroblasts

Transforming growth factor(s) production enables cells to grow in the absence of serum: an autocrine system.

Contact Info

Product

Resources

About