Conditions for induction of alkaline phosphatase in cultured human fetal skin fibroblasts

Lundgren, Erik

doi:10.1016/0014-4827(77)90265-8

Cited by 17 publications

(7 citation statements)

References 11 publications

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“…AlkPase is a rather nonspecific enzyme with many molecular forms and probably many functions (26,27). In intestine and kidney, Alk-Pase has been related to the sodium pump and associated with the rapid passage of ions across cell membranes (27).…”

Section: Discussionmentioning

confidence: 99%

“…When correlating the morphological findings of this study with the different functions which have been hypothesized for Alk-Pase throughout the body (27,28), the following biological roles of the A1-RC may be postulated: (a) The membrane-bound reaction product of Alk-Pase may be related to the production of intercellular fibrillar material which in this study was consistently observed in the vicinity of the AI-RC. It is well known that Alk-Pase can be induced in cultured fibroblasts (26), and that this enzyme may be indicative of collagen synthesis (18,(26)(27)(28)(29). The possibility that reticulum cells are the source of the intercellular reticulum fibers in the bone marrow has long been postulated (30), and was re-emphasized by Weiss (4).…”

Section: Discussionmentioning

confidence: 99%

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Association of alkaline-phosphatase-positive reticulum cells in bone marrow with granulocytic precursors.

Westen¹,

Bainton²

1979

The Journal of Experimental Medicine

218

103

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In the bone marrow, an elaborate stroma forms the structural basis of the hemopoietic microenvironment. In this study, two different types of stromal cells were identified with certainty on tissue sections of intact bone marrow of rats and mice using light and electron microscopic histochemistry: (a) a fibroblast-type of reticulum cell which is characterized by having alkaline phosphatase associated with its plasma membrane. We refer to this cell as the alkaline-phosphatase-positive reticulum cell (Al-RC). It is closely associated with granulocytic precursors, particularly myeloblasts and neutrophilic promyelocytes. These reticulum cells may be found throughout the marrow but are concentrated near the endosteum. (b) a macrophage-type of reticulum cell which is characterized by its abundance of lysosomal acid phosphatase and is mainly associated with erythroid precursors (as observed by others). In contrast to the above-mentioned cell type, this latter cell was found to be distributed uniformly throughout the marrow. We speculate that the Al-RC are mesenchymal stromal cells necessary for granulocytic differentiation in bone marrow.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Association of alkaline-phosphatase-positive reticulum cells in bone marrow with granulocytic precursors.

Westen¹,

Bainton²

1979

The Journal of Experimental Medicine

218

103

View full text Add to dashboard Cite

show abstract

“…In recognition of the heterogeneity of bone marrow stromal cell (24)(25)(26)(27). populations, we have developed flow cytometric methods to distinguish cells according to their level of AP expression.…”

Section: Introductionmentioning

confidence: 99%

Direct flow cytometric quantification of alkaline phosphatase activity in rat bone marrow stromal cells.

Kamalia

McCulloch²,

Tenenbaum³

et al. 1992

J Histochem Cytochem.

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Cell preparations in cytochemistry are conventionally analyzed with transmitted light after fixation and reaction with agents such as azo-coupling dyes. With cell suspensions stained with fluorescent cytochemical dyes, cells can also be analyzed and sorted by flow cytometry. We have exploited the intense red fluorescence of Fast Red Violet LB generated in cytochemical reactions to perform flow cytometric analyses of alkaline phosphatase (AP) expression in rat bone marrow stromal cells. By modifying staining protocols of single-cell suspensions, we demonstrate that in comparison to staining with Fast Red TR, the method is specific, can distinguish among various levels of enzyme expression within the whole population, and permits enzyme kinetic studies of heterogeneous cell populations. The method was applied to study the effect of the glucocorticoid dexamethasone (Dx) on cell proliferation and AP expression. In low AP-expressing cells, Dx treatment at 10(-8) M increased the [3H]-thymidine labeling index from 3.85% to 5.24% (p less than 0.01). In contrast, high AP-expressing cells were unlabeled by [3H]-thymidine. The staining and analytical methods reported here facilitate the detection, isolation, and quantification of subpopulations of bone marrow stromal cells that express alkaline phosphatase activity. These experiments demonstrate the value of flow cytometry as an adjunct to conventional cytochemical methods.

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“…A similar finding was made in human bone marrow-derived stromal cells (Bentley and Foidart, 1980). ST-1 cells are alkaline phosphatase negative, probably because they are routinely cultured in the absence of added hydrocortisone, a n inducer of this enzyme (Lundgren, 1977).…”

Section: Discussionmentioning

confidence: 99%

Isolation of a human stromal cell strain secreting hemopoietic growth factors

Tsai

Emerson

Sieff

et al. 1986

Journal Cellular Physiology

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A diploid fibroblastoid cell strain, termed "ST-1," has been established from a long-term liquid culture of human fetal liver cells. ST-1 cells are nonphagocytic, nonspecific esterase negative and do not possess factor VIII-related antigen but stain with antibodies specific for fibronectin and type I collagen. The ST-1 cells produce nondialyzable hemopoietic growth factors capable of stimulating the development of erythroid bursts, mixed granulocyte-macrophage colonies, pure granulocyte colonies, and pure macrophage colonies. These factors are active on both human fetal liver and human adult bone marrow progenitors. When liquid cultures of human fetal liver hemopoietic progenitors are established with a preformed monolayer of ST-1 cells, the yields of nonadherent cells, erythroid progenitors, and myeloid progenitors are greatly increased. These studies demonstrate that the fibroblastoid ST-1 cells support hemopoiesis in vitro and may be a critical element in the stromal microenviroment in vivo.

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Conditions for induction of alkaline phosphatase in cultured human fetal skin fibroblasts

Cited by 17 publications

References 11 publications

Association of alkaline-phosphatase-positive reticulum cells in bone marrow with granulocytic precursors.

Association of alkaline-phosphatase-positive reticulum cells in bone marrow with granulocytic precursors.

Direct flow cytometric quantification of alkaline phosphatase activity in rat bone marrow stromal cells.

Isolation of a human stromal cell strain secreting hemopoietic growth factors

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