Conditioned medium obtained from mesenchymal stem cells attenuates focal cerebral ischemia reperfusion injury through activation of ERK1/ERK2-BDNF signaling pathway

Aboutaleb, Nahid; Faezi, Masoumeh; Maleki, Solmaz Nasseri; Nazarinia, Donya; Tousi, Seyed Mohammad Taghi Razavi; Hashemirad, Narjes

doi:10.1016/j.jchemneu.2019.02.003

Cited by 28 publications

(18 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been shown that conditioned medium obtained from MSCs has therapeutic effects in several somatic disorders [ 140 , 141 ]. Conditioned medium from DPSCs and SHEDs have also been clarified to have regenerative potential [ 142 , 143 , 144 , 145 ].…”

Section: Regenerative Capacity Of Dpscs and Shedsmentioning

confidence: 99%

Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy

Yoshida

Tomokiyo

Hasegawa

et al. 2020

Biology

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) have the capacity for self-renewal and multilineage differentiation potential, and are considered a promising cell population for cell-based therapy and tissue regeneration. MSCs are isolated from various organs including dental pulp, which originates from cranial neural crest-derived ectomesenchyme. Recently, dental pulp stem cells (DPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) have been isolated from dental pulp tissue of adult permanent teeth and deciduous teeth, respectively. Because of their MSC-like characteristics such as high growth capacity, multipotency, expression of MSC-related markers, and immunomodulatory effects, they are suggested to be an important cell source for tissue regeneration. Here, we review the features of these cells, their potential to regenerate damaged tissues, and the recently acquired understanding of their potential for clinical application in regenerative medicine.

show abstract

Section: Regenerative Capacity Of Dpscs and Shedsmentioning

confidence: 99%

Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy

Yoshida

Tomokiyo

Hasegawa

et al. 2020

Biology

View full text Add to dashboard Cite

show abstract

“…Interestingly, previous studies reported that the activation of ERK1 and ERK2 cascade (46) and vascular endothelial growth factor signaling pathway (47) were associated with the attenuation of I/R injury. These results showed that the application of Custodiol-supplemented with hypoxic CM-BMSCs for donor heart preservation protected graft against I/R injury via different signal pathways.…”

Section: Discussionmentioning

confidence: 99%

Donor Heart Preservation with Hypoxic Conditioned Medium Derived from Bone Marrow Mesenchymal Stem cell improves Cardiac Function in a Heart Transplantation Model

Zhou

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: In heart transplantation, donor hearts inevitably suffer from ischemia/reperfusion (I/R) injury, which leads to primary graft dysfunction and affects patients’ survival rate. Bone marrow mesenchymal stem cells (BMSCs) have been reported to attenuate myocardial I/R injury via their paracrine effects, which can be enhanced by hypoxic preconditioning. We hypothesized that the donor heart preservation with hypoxic conditioned medium derived from BMSCs (CM-BMSCs) would improve post-transplant graft function. Methods: Normoxic CM and hypoxic CM were isolated from rat BMSCs cultured under normoxic (20% O2) or hypoxic (1% O2) condition. Donor hearts were explanted, stored in cardioplegic solution supplemented with either a medium (Vehicle), normoxic CM (N-CM), or hypoxic CM (H-CM), and then heterotopically transplanted. Antibody arrays were performed to compare the differences between hypoxic CM and normoxic CM.Results: After heart transplantation, the donor heart preservation with normoxic CM was associated with shorter re-beating time, histopathological scores, and left ventricular systolic diameter, higher ejection fraction, and fractional shortening of transplanted hearts. These protective effects may be associated with the inhibition of apoptosis and inflammation, as reflected by less TUNEL-positive cells and lower levels of serum proinflammatory cytokines (Interleukin-1β, Interleukin-6, tumor necrosis factor-α) and cardiac troponin I in the N-CM group compared with the vehicle group. These therapeutic effects can be further enhanced by hypoxic preconditioning. Antibody arrays revealed that nine proteins were significantly increased in hypoxic CM compared with normoxic CM. Furthermore, compared with vehicle and N-CM groups, the protein levels of PI3K and p‐Akt/Akt ratio in the transplanted hearts significantly increased in the H-CM group. Conclusions: Our results indicate that cardioplegic solution-enriched with hypoxic CM-BMSCs can be a novel and promising preservation solution for donor hearts.

show abstract

“…This is a unique and intrinsic property of all MSC regardless their tissue origin [76], although tissue source influences the stromal secretome [77]. Administration of MSC after a vascular insult enhances a functional revascularization, associated with the upregulation of CD31 [78,79,80], the activation of anti-apoptotic and pro-survival molecular pathways (caspase-3, Bcl-2, Bcl-xL, Akt), a parallel production of a wide range of soluble mediators (VEGF, NGF, HGF, bFGF, IL-6, IL-8, IL-10), and MSC immunosuppressive properties by secreting HLA-G5 [81,82,83,84,85,86,87,88]. In Vitro experiments have confirmed these results, as crossroads of main survival signaling pathways, like ERK1/2, BDNF, CREB or MAP kinases, are targeted and activated [86,88].…”

Section: Challenges Facing Angiogenesis Bone Healing/regenerationmentioning

confidence: 99%

Substantial Overview on Mesenchymal Stem Cell Biological and Physical Properties as an Opportunity in Translational Medicine

Abdelrazik

Giordano

Bròdano

et al. 2019

IJMS

View full text Add to dashboard Cite

Mesenchymal stem cells (MSC) have piqued worldwide interest for their extensive potential to treat a large array of clinical indications, their unique and controversial immunogenic and immune modulatory properties allowing ample discussions and debates for their possible applications. Emerging data demonstrating that the interaction of biomaterials and physical cues with MSC can guide their differentiation into specific cell lineages also provide new interesting insights for further MSC manipulation in different clinical applications. Moreover, recent discoveries of some regulatory molecules and signaling pathways in MSC niche that may regulate cell fate to distinct lineage herald breakthroughs in regenerative medicine. Although the advancement and success in the MSC field had led to an enormous increase in the amount of ongoing clinical trials, we still lack defined clinical therapeutic protocols. This review will explore the exciting opportunities offered by human and animal MSC, describing relevant biological properties of these cells in the light of the novel emerging evidence mentioned above while addressing the limitations and challenges MSC are still facing.

show abstract

Conditioned medium obtained from mesenchymal stem cells attenuates focal cerebral ischemia reperfusion injury through activation of ERK1/ERK2-BDNF signaling pathway

Cited by 28 publications

References 35 publications

Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy

Insight into the Role of Dental Pulp Stem Cells in Regenerative Therapy

Donor Heart Preservation with Hypoxic Conditioned Medium Derived from Bone Marrow Mesenchymal Stem cell improves Cardiac Function in a Heart Transplantation Model

Substantial Overview on Mesenchymal Stem Cell Biological and Physical Properties as an Opportunity in Translational Medicine

Contact Info

Product

Resources

About