Condensed Tannins Content and their Influence on the Antioxidant Activity of Bark Hydroethanol Extract of Piliostigma reticulatum (Dc) Hochst and its Fractions

Abstract

Piliostigma reticulatum (DC) Hochst (Caesalpiniaceae ; Synonym: Bauhinia reticulata) is a plant that grows in the Sahel-Sudan area, from Senegal to Sudan. It is a plant well known to traditional practitioners in Senegal. In Africa, among its organs, leaves and bark are the most used. These are often used against many diseases, such as ulcers 15 , boils, sores, syphilitic cancer, toothache, gingivitis and diarrhea. 16,17 The leaves are used for their ABSTRACT Background: Consumption of natural products from plants is implicated in the reduction of the occurrence of diseases related to oxidative stress. Piliostigma reticulatum is a plant well known to traditional practitioners in Senegal where leaves and bark are often used against many diseases, such as ulcers, boils, syphilitic cancer, toothache, gingivitis and diarrhea. Aim: This study compared hydroethanol extract from the plant bark and its fractions by assessing their total phenol contents, antioxidant activity and the influence of condensed tannins on their activity. Method: barks were extracted by a moderate decoction with ethanol-water followed by a silica column fractionation with successively ethyl acetate, methanol and water. For this study, assays were carried out before and after precipitation of tannins by BSA and PVPP tests. Total phenol and condensed tannins of hydroethanolic extract and its fractions were performed by Folin Ciocalteu and hydrolysis methods. Antioxidant activity was evaluated by DPPH and CUPRAC tests. Results: Tannins precipitation leads a light decrease of total phenol and condensed tannins contents of samples. Total phenol content of hydroethanolic extract was 51.2 mg GAE/g vs 3.2 after BSA test and 1.7 after PVPP test whereas condensed tannins content obtained 72.2% vs 4.2% and 2.3% after precipitation. Antiradical activity was lost following the elimination of tannins with IC 50 : 5.33 ± 0.04 mg/l vs 78.86 ± 0.92 after BSA and > 500 after PVPP. Conclusion: This results showed the condensed tannins would be in charge of antiradical and reducing activities of plant barks and PVPP precipitated much better the tannins from those extracts than BSA.

Mimosa tenuiflora Aqueous Extract: Role of Condensed Tannins in Anti-Aflatoxin B1 Activity in Aspergillus flavus

“…Five mL of each sample were mixed with 5 mL of water and 500 mg of PVPP. After vortexing for 30 s, the samples were maintained at 4 • C for 15 min and then stirred again for 30 s before being centrifuged at 3000× g for 10 min [63]. The supernatant was then collected for further analyses (subfraction Sf).…”

A Comparative Study on the Sustainability of Groundnut Oil Storage Stability using Ethylacetate Extract from Piliostigma reticulatum and Butylated Hydroxyanisole

“…This tree is well-known for its bioactive compounds, such as flavonoids, tannins, and alkaloids, which may be found in its bark, leaves, and roots. It is a useful resource in traditional medicine, with a history of usage in treating a variety of diseases because of these bioactive chemicals [5,6]. Previous research conducted within our research team has confirmed that Piliostigma reticulatum (DC) Hochst is abundant in a diverse array of phenolic compounds, all possessing robust antioxidant properties [7,8].…”

Phytochemical Analysis of Curry Leaf Extract (Murraya koenigii L.) as a Potential Animal Feed and Medicinal Ingredient

“…Tannins are complex organic compounds found in plants as secondary metabolite products. 29,30 Tannins are composed of phenolic compounds which are difficult to crystallize and separate and contain protein which is difficult to precipitate. Tannins also consist of polyphenolic compounds which have antibacterial, antioxidant and astringent activities.…”