Concurrent separation of catecholamines, dihydroxyphenylglycol, vasoactive intestinal peptide, and neuropeptide Y in superfusate and tissue extract

Hunter, Larry W.; Rorie, Duane K.; Yaksh, Tony L.; Tyce, Gertrude M.

doi:10.1016/0003-2697(88)90198-4

Cited by 38 publications

(17 citation statements)

References 31 publications

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“…Creatine kinase (CK) and lactate dehydrogenase activity in plasma was measured by using a commercial kit (Kanto Chemical Co., Tokyo, Japan). Catecholamines were extracted from plasma using alumina and measured by high-performance liquid chromatography using a modification of the procedure described by Hunter et al 10 Plasma cortisol levels were assayed using a competitive solid phase 125 I radioimmunoassay technique (Dainabot Lab, Tokyo, Japan).…”

Section: Il-6 Assaymentioning

confidence: 99%

Plasma IL-6 levels during arm exercise in persons with spinal cord injury

Umemoto

Furusawa²,

Kouda

et al. 2011

Spinal Cord

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Study design: Non-randomized study. Objectives: Previous studies indicated that at least 2-h leg exercise at more than 60% maximum oxygen consumption (VO 2 max) increased plasma interleukin (IL)-6 in able-bodied (AB) subjects. The purpose of the present study was to compare IL-6 response to arm exercise in AB subjects and persons with spinal cord injury (SCI). Setting: Wakayama Medical University in Japan. Methods: Six subjects with SCI between T6 and T10 and seven AB subjects performed 2-h arm crank ergometer exercise at 60%VO 2 max. Plasma catecholamines, IL-6, tumor necrosis factor (TNF)-a and high-sensitivity C-reactive protein (hsCRP) were measured before exercise, 60-min exercise, immediately and 2 h after the completion of exercise. Results: Arm exercise increased myoglobin and plasma IL-6 levels in SCI and AB (Po0.01), but there were no differences in them between the two groups throughout the study. Plasma levels creatine kinase, lactate dehydrogenase, TNF-a and hsCRP did not change throughout the study in both groups. Conclusion: These findings suggest neither significant muscle damage nor inflammatory response during exercise. The increase in plasma IL-6 in SCI was not unexpected, confirming that moderate intensity and relatively long-arm exercise is safe and beneficial for SCI subjects with regard to IL-6 excretion, as in AB subjects.

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Section: Il-6 Assaymentioning

confidence: 99%

Plasma IL-6 levels during arm exercise in persons with spinal cord injury

Umemoto

Furusawa²,

Kouda

et al. 2011

Spinal Cord

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“…EDTA-2K blood samples were prepared on slides and stained using the Wright-Giemsa method to determine the percentage of neutrophils, total lymphocytes and monocytes. Catecholamines were extracted from plasma using alumina and measured by high-performance liquid chromatography using a modification of the procedure described by Hunter et al 14 Plasma cortisol levels were assayed using a competitive solid phase 125 I radioimmunoassay technique (Dainabot Lab., Tokyo). Mononuclear cells (10 6 cells/ml) were incubated at 371C for 3 h, centrifuged and the concentration of PGE 2 in the supernatant was determined by radioimmunoassay (PGE2 Ria kit Dupnt).…”

Section: Other Blood Testsmentioning

confidence: 99%

Attenuation of natural killer cell activity during 2-h exercise in individuals with spinal cord injuries

Ueta

Furusawa²,

Takahashi

et al. 2007

Spinal Cord

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Design: Non-randomized study. Objective: To determine natural killer cell cytotoxic activity (NKCA) to 2-h arm ergometer exercise in persons with spinal cord injuries (SCI) and the underlying mechanism of such response. Setting: University of Occupational and Environmental Health, Japan. Methods: We examined NKCA response to 2-h arm crank ergometer exercise at 60% of maximum oxygen consumption (VO 2max ) in SCI and able-bodied persons. NKCA and plasma concentrations of prostaglandin E 2 (PGE 2 ), adrenaline and cortisol were measured before, during and immediately after the exercise. The study included seven subjects with SCI between Th11 and L4 and six able-bodied persons.Results: NKCA in able-bodied subjects increased (Po0.05) at 60 min of exercise and immediately after the exercise, and remained elevated up to 2 h after exercise. However, NKCA in SCI decreased (Po0.05) immediately after exercise but recovered at 2 h after exercise. Plasma adrenaline in both groups increased significantly (Po0.05) immediately after exercise and returned to baseline level 2 h after the exercise. Plasma cortisol in both groups remained constant throughout the study. In SCI, PGE 2 significantly increased immediately after 2 h exercise and returned to the baseline level 2 h after exercise; however, it remained unchanged during the test in able-bodied subjects. Conclusion: Our results suggested that increase of PGE 2 in SCI partially contributes to NKCA.

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“…Enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA) was used to measure plasma TNF-a concentration. Catecholamines were extracted from plasma using alumina and measured by high-performance liquid chromatography using a modification of the procedure described by Hunter et al 7 …”

Section: Other Blood Testsmentioning

confidence: 99%

Elevation of interleukin-6 and attenuation of tumor necrosis factor-α during wheelchair half marathon in athletes with cervical spinal cord injuries

et al. 2014

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Study design: Nonrandomized study. Objectives: The purpose of this study was to determine the effects of long and intensive exercise on interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-a) in athletes with cervical spinal cord injuries (CSCI). Setting: The 30th Oita International Wheelchair Marathon Race. Methods: Blood samples from six athletes with CSCI and eight athletes with thoracic and lumber spinal cord injuries (SCI) participating in wheelchair half marathon race were collected before the race, immediately after the race and 2 h after the race. IL-6, TNF-a, adrenaline and blood cell counts were measured. Results: Monocyte count remained stable throughout the study in the CSCI group but was significantly high at 2 h after the race in the SCI group. Plasma IL-6 concentrations were significantly elevated immediately after the race in both groups, although the levels in CSCI were significantly lower than in the SCI group. Plasma adrenaline was significantly elevated immediately after the race in the SCI group but recovered at 2 h after the race. In contrast, plasma adrenaline did not change in the CSCI group throughout the study and was significantly lower than in the SCI group. Plasma TNF-a did not change throughout the study in the SCI group compared with a significant decrease at 2 h after the race in the CSCI group. Conclusion: Long and intensive exercise increased IL-6 in the CSCI group despite the small muscle mass and lack of sympathetic nervous system. The post-race fall in plasma TNF-a in the CSCI group could be related to the inhibitory effect of rising IL-6 in the presence of normal monocyte count and stable adrenaline level.

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Concurrent separation of catecholamines, dihydroxyphenylglycol, vasoactive intestinal peptide, and neuropeptide Y in superfusate and tissue extract

Cited by 38 publications

References 31 publications

Plasma IL-6 levels during arm exercise in persons with spinal cord injury

Plasma IL-6 levels during arm exercise in persons with spinal cord injury

Attenuation of natural killer cell activity during 2-h exercise in individuals with spinal cord injuries

Elevation of interleukin-6 and attenuation of tumor necrosis factor-α during wheelchair half marathon in athletes with cervical spinal cord injuries

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