Concurrent regulation of glutamic acid dehydrogenases of Neurospora

Sanwal, B. D.; Lata, Madhu

doi:10.1016/0003-9861(62)90127-3

Cited by 64 publications

(31 citation statements)

References 20 publications

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“…Sanwal and Lata (1962) found that the NADspecific dehydrogenase [L-glutamate: NAD oxidoreductase (deaminating), EC. 1.4.1.2] (NAD-GDH) of wild-type strains was not induced by the addition of 50 mM L-glutamate alone or by the addition of ammonium nitrate.…”

Section: Introductionmentioning

confidence: 99%

Induction of Nad-Specific Glutamate Dehydrogenase In Neurospora Crassa by Addition of Glutamate to The Media

Wn¹

1969

Aust. Jnl. Of Bio. Sci.

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SummaryThere are two glutamate dehydrogenases (GDH) produced by wild-type strains of N. cra88a, one of which is specific for the coenzyme NADP and the other for the coenzyme NAD. The latter enzyme (NAD-GDH) is induced if glutamate is used as the sole carbon and nitrogen source and is induced to a lesser extent if inorganic nitrogen is added. Addition of sucrose to the medium prevents uptake of glutamate and there is no induction of the enzyme.NADP-specific GDH activity is drastically decreased if glutamate is used as the sole carbon and nitrogen source. Addition of inorganic nitrogen causes a smaller decrease of NADP-GDH.If inorganic nitrogen is used as the sole nitrogen source together with sucrose as the carbon source, NADP-GDH activity is maintained for a longer period and NAD-GDH is not induced.

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Section: Introductionmentioning

confidence: 99%

Induction of Nad-Specific Glutamate Dehydrogenase In Neurospora Crassa by Addition of Glutamate to The Media

Wn¹

1969

Aust. Jnl. Of Bio. Sci.

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“…Different amino acids were not equally effective in producing this induetion (Sanwal and Lata 1962). In the present paper the order of decreasing effectiveness was DL-alanine = D-alanine > L-serine > glycine> L-alanine > L-aspartic (Tables 1, 2, 10).…”

Section: Discussionmentioning

confidence: 44%

“…Urea is a most effective inducer ofNAD-GDH (Sanwal and Lata 1962;Stachow and Sanwa11967) and, contrary to the situation with amino acids, results in induction of NAD-GDH in the presence or absence of sucrose (Table 6). If the hypothesis of a critical ratio is correct, then the internal concentrations of amino acids of mycelia incubated in urea plus sucrose must be extremely high in order to reach this critical ratio.…”

Section: Discussionmentioning

confidence: 99%

“…It has long been known that addition of urea to medium N containing sucrose resulted in a very large induction ofNAD-GDH (Sanwal and Lata 1962;Stachowand SanwaI1967). Further experiments were done to test the effect of urea alone and in the presence of sucrose with or without NH!…”

Section: (C) Effect Of Ureamentioning

confidence: 99%

“…NADP-GDH is biosynthetic, while NAD-GDH is oxidative (Sanwal and Lata 1962;Strickland 1969). Incubation of mycelia in media containing L-glutamate or urea resulted in induction of NAD-GDH and this induction was inhibited by the addition of cycloheximide (Strickland 1969).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Glutamate Dehydrogenases in Neurospora Crassa as a Response to Carbohydrates and Amino Acids in the Media

Wn¹

1971

Aust. Jnl. Of Bio. Sci.

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Mycelial pads of N. crassa grown for 48 hr in minimal medium were harvested, washed, and transferred to test media containing a variety of carbon and nitrogen sources. When some amino acids served as the sole carbon source, NAD-GDH was induced and the activity of NADP-GDH declined. Addition of sucrose depressed or prevented induction of NAD-GDH while NADP·GDH activity was maintained. Internal amino acid concentrations increased when mycelial pads were incubated in amino acids that induced NAD-GDH, but these accumulated amino acids were only oxidized in the absence of sucrose. The rate of amino acid accumulation decreased if sucrose was present in the media. A hypothesis is presented that the induction of NAD-GDH and the activity of NADP-GDH are a function of the ratio of amino acids to sucrose or sucrose metabolites or both. Urea was an excellent inducer of NAD-GDH in the presence or absence of sucrose, although the rate of induction was greater in the absence of sucrose. Incubation of mycelial pads in urea also led to extremely high concentrations of amino acids, thus supporting the ratio hypothesis. Mycelial pads incubated in media containing D-alanine accumulated the amino acid very efficiently, but metabolized it very poorly. Nevertheless, there was strong induction of NAD-GDH, indicating that amino acids per se were the compounds involved on one side of the balance. Addition of 58 mM sucrose to the test medium containing D·alanine prevented the induction of NAD-GDH but did not prevent the accumulation of alanine within the mycelium. Incubation of mycelial pads in media containing all combinations of 50 mM NH4Cl and 58 mM sucrose established that NH; enhanced the induction of NAD-GDH. NH; per se did not induce NAD-GDH.

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Multiple Formen Von Enzymen

Wieland¹,

Pfleiderer²

1963

Advances in Enzymology - And Related Areas of Molecular Biology

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Concurrent regulation of glutamic acid dehydrogenases of Neurospora

Cited by 64 publications

References 20 publications

Induction of Nad-Specific Glutamate Dehydrogenase In Neurospora Crassa by Addition of Glutamate to The Media

Induction of Nad-Specific Glutamate Dehydrogenase In Neurospora Crassa by Addition of Glutamate to The Media

Regulation of Glutamate Dehydrogenases in Neurospora Crassa as a Response to Carbohydrates and Amino Acids in the Media

Multiple Formen Von Enzymen

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