Multiple Formen Von Enzymen

Wieland, Theodor; Pfleiderer, Gerhard

doi:10.1002/9780470122709.ch6

Cited by 2 publications

(2 citation statements)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This enzyme is tetrameric and consists of two sub-units, A (or M) and B (or H) which in combination or alone, form five isoenzymes B4, AB3, Az B2, A3 B and A4, LDH1.5, respectively, and are controlled by two different genetic loci (Wieland and Pfleiderer -1963). This enzyme is tetrameric and consists of two sub-units, A (or M) and B (or H) which in combination or alone, form five isoenzymes B4, AB3, Az B2, A3 B and A4, LDH1.5, respectively, and are controlled by two different genetic loci (Wieland and Pfleiderer -1963).…”

Section: Introductionmentioning

confidence: 99%

“…the reversible reaction between lactate and pyruvate, is responsible for maintaining the balance between these two substrates in the system. This enzyme is tetrameric and consists of two sub-units, A (or M) and B (or H) which in combination or alone, form five isoenzymes B4, AB3, Az B2, A3 B and A4, LDH1.5, respectively, and are controlled by two different genetic loci (Wieland and Pfleiderer -1963). However, another LDH isoenzyme (LDH-C4 or LDH-X) believed to belong to another sub-unit under another genetic locus, has been shown to be specific to the testis and spermatozoa (Blanco and Zinkham -1963;Zinkham et al -1964;Baldwin and Temple-Smith -1973;Holmes et al -1973) and to be related to sperm count and motility (Gerez de Burgos et al -1979) by being involved in the energy-supplying metabolic processes (van Dop et al -1977;Storey and Kayne -1977;Gerez de Burgos et al -1978;Blanco -1980).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Changes in Porcine Sperm Lactate Dehydrogenase Isoenzymes During Sperm Maturation

et al. 2009

View full text Add to dashboard Cite

The changes in sperm lactate dehydrogenase (LDH-lactate: NAD oxidoreductase, EC 1.1.1.27) activity and the relative occurrence of LDH isoenzymes during sperm maturation were studied using pubertal German improved Landrace boars. The LDH of spermatozoa liberated from the testis, caput epididymis, proximal and distal corpus epididymidis and cauda epididymidis was spectrophotometrically quantified while the LDH isoenzymes were separated on fine cellulose acetate membrane strips with the Sartorius Sartophor system.The LDH content of sperm dropped drastically as they moved from the testis to the caput epididymidis. Thereafter, only little and insignificant changes were observed. Testicular sperm was composed more of the fastest anodically-migrating isoenzyme (LDH1 ) while with sperm maturation, the least or slowest migrating isoenzymes (LDH4 and 5) became progressively more dominant.This loss in LDH content in sperm and the shifts in the LDH isoenzyme patterns indicate that the development of sperm during maturation is dependent on a delicate balance between lactate and pyruvate, such that the cathodic isoenzymes involved in the anaerobic energy-supplying metabolic processes are sufficiently available for sperm activity and survival. Veranderungen der Lactat-Dehydrogenase-Isoenzyme in Schweine-Spermatozoen wahrend der ReifungsphaseZusammenfassung: Die Veriinderungen der Laktatdehydrogenase (LDH)-Aktivitit und dem Auftreten verschiedener LDH-Isoenzyme wihrend der Passage der miinnlichen Samenzellen durch den Nebenhoden der Deutschen Landrasse wurden untersucht. Die LDH von Spermatozoen, die aus Hoden, Caput, proximalem und distalem Corpus und Cauda epididymidis gewonnen wurden, wurde photometrisch quantifniert, warend die einzelnen Isoenzyme auf Zelluloseacetat-Membranfolien aufgetrennt wurden.Der LDHGehalt der Spermatozoen fie1 beim a e r t r i t t vom Hoden in das Caput epididymidis drastisch ab, danach gab es kaum weitere Veriinderungen. Wihrend im Hoden das am schnellsten in Richtung Anode wandemde Isoenzym (LDHI ) uberwog, gewannen mit zunehmendem Reifezustand die am wenigsten oder am langsamsten wandemden Isoenzyme (LDH4 und LDH5) die Uberhand.Dieser Vedust an LDH und die Verschiebung des Isoenzym-Musters deutet darauf hin, da8 beim ProzeB der Samenreifung das VerhPtnis von Laktat zu Pyruvat eine entschei-Key words: Lactate dehydrogenase -LDH isoenzymes, sperm maturation, LDHboar sperm, LDH andrologia 18 (1986) LDH lsoenzymes 109 dende Roue spielt, und daB von den kathodischen Isoenzymen geniigend vorhanden sind, das Uberleben der Keimzellen unter anaeroben Bedingungen zu gewslhrleisten.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Changes in Porcine Sperm Lactate Dehydrogenase Isoenzymes During Sperm Maturation

et al. 2009

View full text Add to dashboard Cite

show abstract

Enolase: Multiple Molecular Forms in Fish Muscle

Tsuyuki

Wold

1964

Science

View full text Add to dashboard Cite

Starch-gel electrophoresis shows three distinct molecular forms of enolase in each of eight different species of Salmonidae. The three enolases do not appear to be artifacts of isolation, and their electrophoretic patterns are completely reproducible. The patterns are also highly characteristic for each individual species of fish, and together with the overall myogen patterns they represent unequivocal means of taxonomical identification.

show abstract

Multiple Formen Von Enzymen

Cited by 2 publications

References 103 publications

Changes in Porcine Sperm Lactate Dehydrogenase Isoenzymes During Sperm Maturation

Changes in Porcine Sperm Lactate Dehydrogenase Isoenzymes During Sperm Maturation

Enolase: Multiple Molecular Forms in Fish Muscle

Contact Info

Product

Resources

About