Concurrent occurrence of Amp C and Cefotaxime (CTX)-M inclinical isolates of enterobacteriaceae

Varghese, Diji Sara; Sekar, Uma; Shanthi, M; Arunagiri, Kavita; Vishwanathan, Arun; Vidhya, V. G.; Sekar, Balaraman

doi:10.4103/0972-1282.134456

Cited by 2 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chakraborty et al only found the CIT gene among the isolates in their research [ 24 ]. Varghese et al [ 25 ] revealed that the three most prevalent AmpC genotypes are CIT, DHA, and EBC. In contrast to our findings, Govindaswamy et al [ 26 ] found that the most prevalent AmpC genotype among the isolates was the blaFOX gene (21.9%).…”

Section: Discussionmentioning

confidence: 99%

Plasmid-Mediated AmpC (pAmpC) Genotypes Among Uropathogenic Escherichia coli: A Hospital-Based Study From Western Uttar Pradesh

Rehana¹,

Pandey²,

Singh³

2023

Cureus

View full text Add to dashboard Cite

Resistance due to AmpC and extended-spectrum beta (β)-lactamases (ESBLs) in Escherichia coli is an emerging problem worldwide. AmpC enzymes are a subclass of β-lactamases that have a capacity to hydrolyze and deactivate a large range of β-lactam antibiotics, particularly cephalosporins, penicillins, and monobactams, although frequently being susceptible to carbapenems and fourth-generation cephalosporins. The prevalence of plasmid-mediated AmpC (pAmpC) genotypes in uropathogenic E. coli isolates were looked at a tertiary care teaching hospital of Western Uttar Pradesh. Materials and methodsA total of 312 non-repeat clinical E. coli isolates among patients presented with urinary tract infections (UTIs) were investigated by standard microbiological methods. Isolates were screened for the presence of ampC using a cefoxitin (30 µg) disc and confirmed using an inhibitor-based assay. Using multiplex polymerase chain reaction (PCR), six AmpC genotypes, namely, CIT, DHA, EBC, ACC, FOX, and MOX, were genotypically identified. ResultsA total of 152 (48.72%) uropathogenic E. coli isolates tested positive on the cefoxitin screening. Out of which, AmpC production was confirmed in 118/152 (77.63%) using a phenotypic method. In particular, the pAmpC gene was found in 56/152 (36.84%) isolates. CIT was the most common gene detected in this geographical area (57.14 %). Multiple genes, i.e., CIT and FOX, were also detected in 14.29% of the isolates. ConclusionIdentifying AmpC producers is important in routine microbiology laboratory as they are a nosocomial threat requiring strict adherence to infection control protocols. A confirmatory phenotypic test followed by genotypic tests will help in the correct and accurate identification of this resistance.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasmid-Mediated AmpC (pAmpC) Genotypes Among Uropathogenic Escherichia coli: A Hospital-Based Study From Western Uttar Pradesh

Rehana¹,

Pandey²,

Singh³

2023

Cureus

View full text Add to dashboard Cite

show abstract

Molecular Characterization of AmpC β-lactamases in Enterobacteriaceae

Bindu¹,

Saikumar²

2022

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

AmpC β-lactamases are enzymes that are resistant to β-lactams, such as penicillin and cephalosporin, but not cefoxitin and cefotetan. This study was conducted to characterize AmpC β-lactamases in Enterobacteriaceae. This study included 200 cephalosporin-resistant Gram-negative isolates recovered from different samples between January 2015 and December 2016. The isolates were subjected to phenotypic tests, and those that tested positive were further analyzed by PCR for six AmpC genotypes: ACC, DHA, FOX, CIT, MOX, and EBC. Among the 200 strains, 32% (64) were positive for AmpC β-lactamases by different phenotypic methods. The target genotypes were detected in 20 (10%) of the isolates. Pus was the predominant source of AmpC isolates. Klebsiella pneumoniae (55%) was the most common producer of AmpC β-lactamase. CIT-FOX was the predominant gene type. As there is variation in the prevalence of AmpC β-lactamases in different geographic regions, periodic surveillance and measures to control infection can prevent the spread of these genes.

show abstract

Concurrent occurrence of Amp C and Cefotaxime (CTX)-M inclinical isolates of enterobacteriaceae

Cited by 2 publications

References 17 publications

Plasmid-Mediated AmpC (pAmpC) Genotypes Among Uropathogenic Escherichia coli: A Hospital-Based Study From Western Uttar Pradesh

Plasmid-Mediated AmpC (pAmpC) Genotypes Among Uropathogenic Escherichia coli: A Hospital-Based Study From Western Uttar Pradesh

Molecular Characterization of AmpC β-lactamases in Enterobacteriaceae

Contact Info

Product

Resources

About