Concurrent estimation of some co-administered antimicrobial drugs applying conventional and first derivative synchronous fluorescence spectroscopy techniques

El-Aziz, Heba Abd; Fathy, M. E.; El‐Enany, N.; Aly, Fatma A.; Tolba, M. M.

doi:10.1016/j.saa.2021.120255

Cited by 9 publications

(8 citation statements)

References 34 publications

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“…Evaluating the validation parameters of the proposed method with regard to the reference methods [41,42] reflected comparable accuracy and linearity, as reflected from recovery percentages and R 2 values respectively (table 4). Meanwhile, the proposed method was superior to the reference methods offering wider linearity ranges for both drugs (table 4), permitting expansion of its application scope.…”

Section: Resultsmentioning

confidence: 94%

“…Through the application of the suggested analytical method to the analytes, and comparing the obtained results with those of a well-characterized assay, the accuracy is evaluated by judging the nearness of the results of both methods [22]. Different concentrations of CIP and LEV were assayed by the proposed method over their linearity ranges, and the obtained data were then compared with those of the reported methods [41,42]. The adequate closeness between both methods was recoded as implemented from the Students' t test and variance ratio F test [43], in addition to the resultant high recovery percentages (table 2) proving the accuracy of the proposed method.…”

Section: Resultsmentioning

confidence: 99%

“…Sensitivity—expressed as LOD and LOQ—was found to be comparable in the case of analysing LEV by the proposed or the reference method [42] (table 4). On the other hand, LOD and LOQ for CIP using the reference method [41] (table 4) imply that it is more sensitive than the suggested method; however, the latter presents more reliable values. Ermer and Miller evaluated different approaches for calculating LOD and LOQ values in their book concerned with validation in pharmaceutical analysis [44].…”

Section: Resultsmentioning

confidence: 99%

Section: Analytical Methods Validationmentioning

confidence: 99%

“…The proposed method followed the validation parameters stated by the USP [22] which adopted the aforementioned approach to evaluate sensitivity in terms of LOD and LOQ as previously described. On the other hand, the reference method for CIP [41] followed the International Council for Harmonisation guidelines [45], which is expected to result in unsubstantial sensitivity parameters since spiking was not carried out. These facts explain the accordance in sensitivity for LEV analysis by both the proposed and reference methods [42], which also followed signal to noise ratio approach to evaluate sensitivity.…”

Section: Analytical Methods Validationmentioning

confidence: 99%

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Green approach for tracking the photofate of ciprofloxacin and levofloxacin in different matrices adopting synchronous fluorescence spectroscopy: a kinetic study

et al. 2023

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First derivative synchronous fluorescence spectroscopy (FDSFS) was applied to detect and quantify either ciprofloxacin (CIP) or levofloxacin (LEV) simultaneously with their photodegradation products, where the photolytic pathway for each analyte was found to be pH dependent. Under the guidance of early published articles, the structure of the produced photolytic products could be concluded, and further related to their resultant fluorescence spectra. The proposed method was subjected to full validation procedure which enables its application in investigating the photodegradation kinetics for both drugs. The obtained kinetic parameters were in accordance with previous reports and could be linked to predict the antibacterial activity of the resultant photodegradation products. These facts prove the suitability of the suggested FDSFS to serve as a stability-indicating assay method and to trace the photofate of CIP and LEV in the ecosystem as potential contaminants. Furthermore, the greenness of the suggested analytical methodology was evaluated via ‘Green Analytical Procedure Index’ (GAPI), which classifies it as an eco-friendly assay. Eventually, no extraction, treatment or preparation steps were needed during all analysis steps, which renders the proposed assay an appealing tool in environmental analysis.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Analytical Methods Validationmentioning

confidence: 99%

Section: Analytical Methods Validationmentioning

confidence: 99%

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Green approach for tracking the photofate of ciprofloxacin and levofloxacin in different matrices adopting synchronous fluorescence spectroscopy: a kinetic study

et al. 2023

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Green micellar factorial design optimized first derivative synchronous spectrofluorimetric method for tripelennamine and diphenhydramine determination in pharmaceutical gel

Amer,

El‐Khateeb

2024

Luminescence

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A green micellar synchronous spectrofluorimetric method was developed and validated for simultaneous determination of tripelennamine hydrochloride and diphenhydramine in bulk and combined pharmaceutical formulation. Synchronous fluorescence of tripelennamine hydrochloride and diphenhydramine was determined using Δλ = 60 nm. The first derivative of synchronous fluorescence was computed to resolve overlap in the synchronous fluorescence spectra. Tripelennamine hydrochloride was quantified at 375 nm, whereas diphenhydramine was quantified at 293 nm; each is the zero‐crossing point of the other. As diphenhydramine exhibited weak native fluorescence, micelle enhancement upon incorporation of sodium dodecyl sulfate was considered. Two‐level full factorial design was carried out to optimize experimental parameters. Optimum conditions involved using SDS (2% w/v) along with Teorell and Stenhagen buffer (pH 9). The method was found to be linear over the range 0.2–4.5 and 0.2–5 μg/mL for tripelennamine and diphenhydramine, respectively, with limits of detection 0.211 and 0.159 μg/mL. The method was successfully applied for simultaneous determination of tripelennamine hydrochloride and diphenhydramine in laboratory‐prepared gel containing all possible excipients with mean percent recoveries ±SD 100.59 ± 0.79 and 98.99 ± 0.98 for tripelennamine hydrochloride and diphenhydramine, respectively. The proposed method was proved to be eco‐friendly using different greenness assessment tools.

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Electroanalytical application of Ag@POM@rGO nanocomposite and ionic liquid modified carbon paste electrode for the quantification of ciprofloxacin antibiotic

Shafiei

Hassaninejad–Darzi

2023

Journal of Electroanalytical Chemistry

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Concurrent estimation of some co-administered antimicrobial drugs applying conventional and first derivative synchronous fluorescence spectroscopy techniques

Cited by 9 publications

References 34 publications

Green approach for tracking the photofate of ciprofloxacin and levofloxacin in different matrices adopting synchronous fluorescence spectroscopy: a kinetic study

Green approach for tracking the photofate of ciprofloxacin and levofloxacin in different matrices adopting synchronous fluorescence spectroscopy: a kinetic study

Green micellar factorial design optimized first derivative synchronous spectrofluorimetric method for tripelennamine and diphenhydramine determination in pharmaceutical gel

Electroanalytical application of Ag@POM@rGO nanocomposite and ionic liquid modified carbon paste electrode for the quantification of ciprofloxacin antibiotic

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