Concordance of urinary microbiota detected by 16S ribosomal RNA amplicon sequencing vs expanded quantitative urine culture

Vaughan, Monique H.; Zemtsov, Gregory; Dahl, Erin M; Karstens, Lisa; Ma, Li; Siddiqui, Nazema Y.

doi:10.1016/j.ajog.2022.06.031

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…Additionally, it is important to critically evaluate and identify gaps in current benchmarking and optimization studies for pediatric urobiome applications. For example, many of the laboratory methods have been evaluated for optimization on adult human and canine urines ( 88 – 90 ), and several comparisons of detection methods ( 91 ), and bioinformatics ( 38 , 41 ) specifically for the urobiome. It is also essential that investigators in the pediatric urobiome research community employ current recommendations developed by the urobiome ( 15 ), kidney microbiome ( 92 ), and general microbiome communities ( 93 ) and develop consensus on standards and guidelines for the field.…”

Section: Larger Gaps To Be Addressed In Future Studiesmentioning

confidence: 99%

Synthesis of current pediatric urinary microbiome research

Jeries,

Sysoeva,

Karstens

et al. 2024

Front. Pediatr.

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The human urinary bladder hosts a complex microbial community of low biomass referred to as the urobiome. While the composition of the urobiome has been investigated in adults for over a decade now, only a few studies have considered the presence and composition of the urobiome in children. It is critical to explore how the urobiome develops throughout the life span and how it changes in the presence of various health conditions. Therefore, we set to review the available data on pediatric urobiome composition and its development with age and disease. In addition, we focused on identifying and reporting specific gaps in our knowledge of the pediatric urobiome that we hope will be addressed by future studies in this swiftly developing field with fast-improving methods and consensus.

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Section: Larger Gaps To Be Addressed In Future Studiesmentioning

confidence: 99%

Synthesis of current pediatric urinary microbiome research

Jeries,

Sysoeva,

Karstens

et al. 2024

Front. Pediatr.

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“…Women with active UTI. 59 Females Not specified (Vaughan et al, 2022) medication (Halverson et al, 2022b). Taken together, findings of these studies have indicated that UUI medications affect patients' microbial profiles differently and further studies are warranted to determine the mechanisms of action of these medications in regards to urobiome.…”

mentioning

confidence: 98%

Clinical relevance of expanded quantitative urine culture in health and disease

Deen

Ahmed

Tasnim

et al. 2023

Front. Cell. Infect. Microbiol.

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“Expanded quantitative urine culture (EQUC)” is an enhanced culture protocol for the detection of viable microbes in urine specimens. Using a large volume of urine and different sets of cultural conditions, EQUC is able to uncover a wide range of bacteria and fungi (yeasts) that were otherwise undetected by the standard urinary culture. In addition to common urinary pathogens, EQUC has been shown to detect emerging and new pathogens, and commensal microbiota. Although the usefulness of EQUC protocol in clinical set up has not yet been fully established, recent studies have demonstrated that EQUC can provide valuable information regarding symptom resolution, treatment responses and diagnosis of major urinary disorders including urinary tract infections, urinary incontinence and other lower urinary tract symptoms. EQUC may also help in evaluating the utility of beneficial microbiota as biotherapeutics. This narrative minireview describes the current research findings regarding the clinical utility of EQUC in characterizing the role of urinary microbiome and uropathogens in health and disease. The literature which are written in English, available on “PubMed” and contain any of the terms- “expanded quantitative urine culture”, “enhanced quantitative urine culture” and “EQUC” in the abstracts were used as the source articles to prepare this minireview.

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