Conceptual Design of Soft X-ray Microscopy for Live Biological Samples

Kim, Kyong-Woo; Nam, Ki-Yong; Kwon, Youngman; Shim, Seong-Taek; Kim, Kyu-Gyeom; Yoon, Kwon‐Ha

doi:10.3807/josk.2003.7.4.230

Cited by 12 publications

(5 citation statements)

References 3 publications

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“…[9][10] For the characterization of the X-ray source, wavelength (or energy) spectrum and its intensity were presented in this paper. In addition, we summarized the results of the imaging test.…”

Section: Discussionmentioning

confidence: 99%

Development of laser plasma-based soft x-ray microscopy

Lim

Nam

Kim

et al. 2005

Laser-Generated, Synchrotron, and Other Laboratory X-Ray and EUV Sources, Optics, and Applications II

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A compact soft X-ray microscope system has been developed for biological applications with nano-scale resolution. Soft X-ray used to the system is emitted from a solid target by using Nd-YAG pulsed laser. Boron nitride (BN) is used as the target materials in the system. The optics of the microscope system is adopted with wolter type-I mirrors, which is consisted of a condenser mirror with demagnification of 1/4× and an object mirror with magnification of 32×. The surface roughness of the machined wolter mirrors is about 0.8 nm (Ra) after polishing. In this paper, the X-ray characteristics, i.e., spectrum and intensity emitted from laser plasma-based x-ray source was measured. Imaging test using the system was performed with gold 2000 mesh. The spatial resolution of the soft x-ray microscope system was obtained about 900 nm.

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Section: Discussionmentioning

confidence: 99%

Development of laser plasma-based soft x-ray microscopy

Lim

Nam

Kim

et al. 2005

Laser-Generated, Synchrotron, and Other Laboratory X-Ray and EUV Sources, Optics, and Applications II

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“…В этом случае влажный образец помещается между двух пленок, что снижает скорость испарения жидкости, но за счет прохода излучения через две пленки увеличиваются потери на рабочих длинах волн. Чаще всего в микроскопии в спектральном диапазоне " водного окна" с этой целью используются Si 3 N 4 -мембраны толщиной 100 nm и коэффициентом пропускания 50−70% за один проход [15]. Как будет показано далее, за счет выбора более прозрачного материала при той же толщине пленки можно достичь коэффициента пропускания до 85%.…”

Section: международный симпозиум "unclassified

Поиск прочных многослойных композиций свободновисящих фильтров с высоким коэффициентом пропускания в диапазоне длин волн "водного окна" (2.3-4.4 nm)

Барышева¹,

Гарахин²,

Лопатин³

et al. 2022

Журнал Технической Физики

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Some variants of the composition of multilayer absorption film filters with a high transmittance in the spectral region of the “water window” (2.3–4.4 nm) have been considered. Having created an ultimate pressure difference between the sides of free-standing films at which they are damaged, we compared the strength of 100 nm thick Ti-based multilayer filters with Al, Be, C interlayers and 100 nm thick V-based multilayer filters with Al interlayers. Sc and Cr was also considered as interlayers. Among the tested periodic multilayer structures, the best strength characteristics were demonstrated by Ti/Be (with a fraction of Ti in a period of about 0.6) and V/Al (with a fraction of V in a period of about 0.4) multilayer filters. Despite the fact that Ti/Be and V/Al filters are inferior in strength to Ti and V monolayer filters of the same thickness, these multilayer filters may be of interest, since they have either a higher transmittance in the “water window” (Ti/Be) or more high level of blocking of visible radiation (V/Al).

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“…In this case, a wet sample is placed between two films, which reduces the evaporation rate of the liquid, but due to the passage of radiation through two films, losses at working wavelengths increase. Most often, in microscopy in the spectral range " of the water window" Si 3 N 4 -membranes with a thickness of 100 nm and a transmittance of 50−70% in one pass [15] are used for this purpose. As will be shown below, by choosing a more transparent material with the same film thickness, a transmittance of up to 85% can be achieved.…”

Section: Introductionmentioning

confidence: 99%

Search for high-strength multilayer free-standing film filters with high transmittance in the wavelength range of the "water window" (2.3-4.4 nm)

et al. 2022

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Some variants of the composition of multilayer absorption film filters with a high transmittance in the spectral region of the "water window" (2.3-4.4 nm) have been considered. Having created an ultimate pressure difference between the sides of free-standing films at which they are damaged, we compared the strength of 100 nm thick Ti-based multilayer filters with Al, Be, C interlayers and 100 nm thick V-based multilayer filters with Al interlayers. Sc and Cr was also considered as interlayers. Among the tested periodic multilayer structures, the best strength characteristics were demonstrated by Ti/Be (with a fraction of Ti in a period of about 0.6) and V/Al (with a fraction of V in a period of about 0.4) multilayer filters. Despite the fact that Ti/Be and V/Al filters are inferior in strength to Ti and V monolayer filters of the same thickness, these multilayer filters may be of interest, since they have either a higher transmittance in the "water window" (Ti/Be) or more high level of blocking of visible radiation (V/Al). Keywords: spectral region of the "water window" multilayer free-standing filters, ultimate pressure difference, film filter strength, transmittance in the soft x-ray, visible light blocking level.

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Conceptual Design of Soft X-ray Microscopy for Live Biological Samples

Cited by 12 publications

References 3 publications

Development of laser plasma-based soft x-ray microscopy

Development of laser plasma-based soft x-ray microscopy

Поиск прочных многослойных композиций свободновисящих фильтров с высоким коэффициентом пропускания в диапазоне длин волн "водного окна" (2.3-4.4 nm)

Search for high-strength multilayer free-standing film filters with high transmittance in the wavelength range of the "water window" (2.3-4.4 nm)

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Conceptual Design of Soft X-ray Microscopy for Live Biological Samples

Cited by 12 publications

References 3 publications

Development of laser plasma-based soft x-ray microscopy

Development of laser plasma-based soft x-ray microscopy

Поиск прочных многослойных композиций свободновисящих фильтров с высоким коэффициентом пропускания в диапазоне длин волн "водного окна" (2.3-4.4 nm)

Search for high-strength multilayer free-standing film filters with high transmittance in the wavelength range of the &quot;water window&quot; (2.3-4.4 nm)

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Product

Resources

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Search for high-strength multilayer free-standing film filters with high transmittance in the wavelength range of the "water window" (2.3-4.4 nm)