This study was aimed to analyze the problem solving ability of high school students in biology learning. The method used was descriptive-quantitative. Data collection techniques used was problem solving essay test. The subjects were high school students grade XI in Surakarta which are divided into A category (high class) and B category (low grade). Based on the analysis, it can be known that the achievement of problem solving abilities in grade XI, A category includes: define the problem (41.67%), examine the problem (40%), plan the solutions (18.39%), implement plans that has been made (34.76 %) and evaluate (42.20%); while B category: define the problem (46.35%), examine the problem (33.89%), plan the solutions (15.78%), implement plans that have been made (20%) and evaluate (33.33%). The lowest percentage in both categories is found in planning solutions and evaluating. Based on the results, the average problem solving ability in A category is 35.40%, and B category is 29.87%. Based on the data, it can be concluded that students' problem solving abilities in A category and B category were still low. This ability can be improved by doing learning oriented to problem solving and familiarizing the students to solve problems in accordance with the stages of problem solving.