Concentrations of Some Metabolites in the Aqueous Humour of Human Senile Cataractous Eyes

Laursen, A. Bruun

doi:10.1111/j.1755-3768.1975.tb01169.x

Cited by 5 publications

(3 citation statements)

References 4 publications

(5 reference statements)

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“…A number of studies evaluated nondiabetic individuals and reported the AHG level was found to decrease with increasing age. 14 Their findings may explain the results in our study. However, the patients in our study did not have a large age span.…”

Section: Discussionsupporting

confidence: 70%

“…13,14 Due to the scientific and technological level of the experiment at that time and the limited number of samples, these data results vary from paper to paper. The earlist study that evaluated diabetic patients was published by Laursen et al 14 They reported the levels of metabolites in AH of five fasting DC patients.In recent studies, an increased oxidative stress, perturbations in amino acid metabolism, and changes in the lipid profile in AH among DC patients were found. 15,16 However, glucose metabolites have been less studied.…”

Section: Discussionmentioning

confidence: 99%

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Concentrations of glucose metabolites in the aqueous humour of diabetic cataract eyes

Ren

Zhang

Yang

et al. 2023

European Journal of Ophthalmology

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Purpose Glucose metabolism underpins diabetic cataracts (DCs), but the relationship between the two remains unclear. Here, we tested the aqueous humour (AH) of patients with DCs to elucidate glucose metabolite levels. Methods In this study, aqueous humour (AH) samples were collected preoperatively from DC eyes (n = 37) and age-related cataract eyes (n = 37) from 74 patients (74 eyes) undergoing uncomplicated cataract surgery. The content of glucose, pyruvate, L-lactate were detected by biochemical methods and advanced glycation end-products (AGEs) was detemined using enzyme-linked immunosorbent assay (ELISA) technique. Furthermore, the ratios of glucose/pyruvate and L-lactate/pyruvate in the AH were calculated. In addition, we calculated the correlation between glucose levels and AGEs in the AH. Results The concentrations of glucose, pyruvate and AGEs in the DC group were higher than those in the control group. Significantly lower levels of L-lactate in the AH were found in the DC group. We calculated the glucose/pyruvate ratio and the L-lactate/pyruvate ratio in the AH, which showed that glucose metabolism was changed in the AH from DC patients. Interestingly, we observed that AGEs in the AH were significantly correlated with increased anterior chamber glucose permeability. A stronger correlation was found in the subgroups of male patients, younger patients, and patients with poor glycaemic control status. Conclusions Comparison of the levels of glucose metabolism-related products in the AH in the DC group highlight a potential pathological mechanism for DC from a glucose metabolism perspective. The findings indicated an alteration in the metabolic pathways of energy metabolism and amino acids in the AH of DC patients.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Concentrations of glucose metabolites in the aqueous humour of diabetic cataract eyes

Ren

Zhang

Yang

et al. 2023

European Journal of Ophthalmology

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“…Accuracy and reproducibility for L-lactate determinations in aqueous humour were investigated by Bruun Laursen & Lorentzen (1975) and by Bruun Laursen (1975). Buffer pH 8.5 was used for the determinations of aqueous humour and lens L-lactate concentrations.…”

Section: A Bruun Laursenmentioning

confidence: 99%

Concentrations of Some Ribonucleotides, L‐lactate, and Pyruvate in Human Senile Cataractous Lenses With Special Reference to Anterior Capsular/Subcapsular Opacity

Laursen

1976

Acta Ophthalmologica

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The concentrations of some ribonucleoside tri- and diphosphates, adenosine-5'-monophosphate, L-lactate and pyruvate were determined in human senile cataractous lenses removed during cataract operations. Pyruvate concentrations were found to be negligible (median = 56 mumol/kg lens wet weight) in 15 human senile cataractous lenses. On the basis of correlations between the biomicroscopic appearances of the senile cataractous lenses (N = 80) and the concentrations and ratios of the metabolites in question, the following classification was found to be justified: 1. Immature cataractous lenses without anterior capsular/subcapsular opacity: high levels of ribonucleoside triphosphates (RTP), high sums of RTP, ribonucleoside diphosphates (RDP), and adenosine 5'-monophosphate (AMP) as well as high levels of L-lactate and high ratios of L-lactate in the lens/L-lactate in the aqueous. 2. Immature cataractous lenses with anterior capsular/subcapsular opacity; intermediate levels of RTP, intermediate values for the sums of RTP, RDP, and AMP, high L-lactate levels, and intermediate values of the ratios of L-lactate in the lens/L-lactate in the aqueous. 3. Totally opaque lenses, which all had extensive anterior capsular/subcapsular opacity; low values for the concentrations of lens RTP, for the sums of RTP, RDP and AMP, and for lens L-lactate. Low ratios of L-lactate in the lens/L-lactate in the aqueous.

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Non‐invasive detection of antibiotics and physiological substances in the aqueous humor by raman spectroscopy

et al. 2006

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This special illumination design gives the opportunity of avoiding the direct exposure to the laser light of basic cordial tissues of the eye, like lens and retina, although an optimum collection of scattered light is accomplished. Concentrations close to the minimum inhibitory concentration (MIC) have been detected for ceftazidime and amphotericin b; the detection of glucose has been realized at concentrations close to the early pathological levels of patients with diabetes.

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Concentrations of Some Metabolites in the Aqueous Humour of Human Senile Cataractous Eyes

Cited by 5 publications

References 4 publications

Concentrations of glucose metabolites in the aqueous humour of diabetic cataract eyes

Concentrations of glucose metabolites in the aqueous humour of diabetic cataract eyes

Concentrations of Some Ribonucleotides, L‐lactate, and Pyruvate in Human Senile Cataractous Lenses With Special Reference to Anterior Capsular/Subcapsular Opacity

Non‐invasive detection of antibiotics and physiological substances in the aqueous humor by raman spectroscopy

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