Concentrations of carotenoids, retinol and alpha-tocopherol in plasma and follicular fluid of women undergoing IVF

Schweigert, Florian J.; Steinhagen, Beate; Raila, Jens; Siemann, Anette; Peet, David; Büscher, Ulrich

doi:10.1093/humrep/deg249

Cited by 83 publications

(70 citation statements)

References 28 publications

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“…These results suggest that antioxidant FF HDL particle components may impact embryo quality during IVF. The presence of both oxidative stress markers [42] and antioxidant molecules and enzymes, especially lipophilic micronutrients [43,44], has been reported in developing human ovarian follicles, implying a dynamic biochemical environment. In our recent pilot work, we measured lipophilic micronutrients in FF and demonstrated their associations with embryo quality indicators [22], supporting a potential antioxidant role in the microenvironment of developing oocytes during IVF.…”

Section: Geometric Mean Concentration (µMol/l) Hdl-c Subfractions (Nm)mentioning

confidence: 99%

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Kim

Bloom

Browne

et al. 2016

J Assist Reprod Genet

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Purpose Follicular redox balance is likely to be important for embryo quality during in vitro fertilization (IVF), and the anti-oxidative high desity lipoprotein (HDL) particle is the sole lipoprotein measured in follicular fluid (FF). Therefore, we investigated FF HDL particle components as predictors of embryo quality during IVF. Methods Two research follicles collected from each participant were individually tracked, and 103 women having at least one developed embryo were included in the analysis. Concentrations of 15 non-cholesterol HDL particle components and 26 HDL-cholesterol (HDL-C) particle size subfractions were determined. Embryo quality was assessed for embryo cell number, embryo fragmentation, and embryo symmetry. Multivariable Poisson regression with a sandwich variance estimator was used to evaluate associations between HDL particle components and embryo quality, adjusted for covariates. Results Higher γ-tocopherol concentration was associated with less embryo fragmentation (relative risk [RR] = 4.43; 95 % confidence interval [CI] 1.78, 11.06), and higher apolipoprotein A-1 concentration was associated with full embryo symmetry (RR = 3.92; 95 % CI 1.56, 9.90). Higher concentrations of HDL-C subfractions in the large and medium particle size ranges were associated with poorer embryo quality. Conclusions FF HDL lipophilic micronutrients and protein components, as well as HDL-C particle size, may be important predictors of embryo quality during IVF.

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Section: Geometric Mean Concentration (µMol/l) Hdl-c Subfractions (Nm)mentioning

confidence: 99%

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Kim

Bloom

Browne

et al. 2016

J Assist Reprod Genet

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“…The supernatants were removed, pooled, and evaporated under nitrogen and reconstituted in 200 Ìl isopropanol and injected into the HPLC system (Waters, Eschborn, Germany). For separation and quantification, a gradient reversed-phase HPLC system was used as described [17]. Accuracy and precision of the analyses were verified using a standard reference material (SMR 968a fat-soluble vitamins in human serum; National Institute of Standards and Technology (NIST), Gaithersburg, Md., USA).…”

Section: Determination Of Vitamin a Vitamin E And Carotenoidsmentioning

confidence: 99%

Accumulation of Selected Carotenoids, α-Tocopherol and Retinol in Human Ovarian Carcinoma Ascitic Fluid

Schweigert

Raila²,

Sehouli³

et al. 2004

Ann Nutr Metab

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Background: Patients with severe forms of cancer are reported to have reduced concentrations of micronutrients in plasma due to the chronic reduction of food intake and an increased metabolism of these components. The purpose of this study was to evaluate if an accumulation of carotenoids, α-tocopherol and retinol in malignant ascitic fluid in women with ovarian cancer might contribute to a loss of these components from plasma. Methods: Blood and ascitic fluid samples obtained from 21 women with ovarian carcinomas and 17 healthy controls were analyzed for retinol, retinol-binding protein (RBP), α-tocopherol and carotenoids. Results: Plasma concentrations of all micronutrients were lower in cancer patients compared to controls. Ascitic fluid concentration of all investigated components was comparable (73–110%) to plasma. While the mean concentration of retinol in malignant ascites represented 73% of that in plasma, the concentration of RBP was less than 10% resulting in an increased mean molar ratio of retinol to RBP from 1.18 to 10.5. Conclusions: The results suggest that lower plasma concentrations of micronutrients in women suffering from ovarian carcinoma are not only caused by a cachexia-induced decrease of food intake and a higher rate of metabolic utilization, but also by a substantial yet not considered transfer from plasma into ascitic fluid possibly associated with plasma lipoproteins. This raises questions with regard to the protective function of these plasma components in ascitic fluid, the consequences of paracentesis on an additional supplementation and finally the possibility to use one or a combination of these components as an additional marker to discriminate between benign and malignant ascites.

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“…Serum was obtained by centrifuging the blood (3,000 g; 10 min), and it was stored at -80°C and analyzed within 3 months. The concentrations of retinol were determined by reversed-phase HPLC (RP-HPLC) as described previously, using a serum volume of 100 µl [18]. RBP4 concentrations were measured via an enzyme-linked immunosorbent assay (ELISA), which requires 10 µl serum for quantification [18].…”

Section: Methodsmentioning

confidence: 99%

The Relative Dose Response Test Based on Retinol-Binding Protein 4 Is Not Suitable to Assess Vitamin A Status in Very Low Birth Weight Infants

et al. 2013

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Background: The relative dose response (RDR) test, which quantifies the increase in serum retinol after vitamin A administration, is a qualitative measure of liver vitamin A stores. Particularly in preterm infants, the feasibility of the RDR test involving blood is critically dependent on small sample volumes. Objectives: This study aimed to assess whether the RDR calculated with retinol-binding protein 4 (RBP4) might be a substitute for the classical retinol-based RDR test for assessing vitamin A status in very preterm infants. Methods: This study included preterm infants with a birth weight below 1,500 g (n = 63, median birth weight 985 g, median gestational age 27.4 weeks) who were treated with 5,000 IU retinyl palmitate intramuscularly 3 times a week for 4 weeks. On day 3 (first vitamin A injection) and day 28 of life (last vitamin A injection), the RDR was calculated and compared using serum retinol and RBP4 concentrations. Results: The concentrations of retinol (p < 0.001) and RBP4 (p < 0.01) increased significantly from day 3 to day 28. On day 3, the median (IQR) retinol-RDR was 27% (8.4-42.5) and the median RBP4-RDR was 8.4% (-3.4 to 27.9), compared to 7.5% (-10.6 to 20.8) and -0.61% (-19.7 to 15.3) on day 28. The results for retinol-RDR and RBP4-RDR revealed no significant correlation. The agreement between retinol-RDR and RBP4-RDR was poor (day 3: Cohen's κ = 0.12; day 28: Cohen's κ = 0.18). Conclusion: The RDR test based on circulating RBP4 is unlikely to reflect the hepatic vitamin A status in preterm infants.

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Concentrations of carotenoids, retinol and alpha-tocopherol in plasma and follicular fluid of women undergoing IVF

Abstract: It remains to be elucidated, if this is indicative of a disturbed sieving effect of the blood-follicle barrier with possibly negative consequences for oocyte maturation.

Cited by 83 publications

References 28 publications

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Associations between follicular fluid high density lipoprotein particle components and embryo quality among in vitro fertilization patients

Accumulation of Selected Carotenoids, α-Tocopherol and Retinol in Human Ovarian Carcinoma Ascitic Fluid

The Relative Dose Response Test Based on Retinol-Binding Protein 4 Is Not Suitable to Assess Vitamin A Status in Very Low Birth Weight Infants

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