Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease

McMichael, Maureen; Ruaux, Craig G.; Baltzer, Wendy I.; Kerwin, Sharon C.; Hosgood, Giselle; Steiner, Jörg M.; Williams, David A.

doi:10.2460/ajvr.67.7.1226

Cited by 25 publications

(33 citation statements)

References 48 publications

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“…The results of this study cannot be compared to other studies, however, mildly increased lipid peroxidation following the intense, short-duration exercise of agility competition occurred in the dogs reported here and was not affected by the speed or training of the dogs. Further research, preferably using GC/NICI-MS for urinary F 2 isoprostane determination, is required to determine the extent of oxidant stress induced by this type of exercise in relation to long duration exercise in sled dogs and to disease states including lung injury [26], neurologic disease [16] and heart disease [27]. Future research is warranted to determine the effect of agility exercise on other parameters of oxidant stress including glutathione, glutathione-peroxidase, catalase, superoxide dismutase.…”

Section: Discussionmentioning

confidence: 99%

“…Intra-assay coefficient of variation ranged from 19.9: at 2 pg/ml to 12.6% at 500 pg/ml and inter-assay coefficient of variation ranged from 9.6% at 2 pg/ml dose to 10.5% at 500 pg/ml. In-house validation was performed with urine samples previously collected from healthy dogs as part of unrelated, previously published research in dogs [16]. Our previous validation of the assay included the assessment of dilutional parallelism of canine urine samples (5 samples at 3 dilutions in which we determined the correct dilution for samples such that they would be within the working range of the assay) and determination of interassay variability (5 samples assayed on 5 separate days) [16].…”

Section: Methodsmentioning

confidence: 99%

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The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs

Baltzer

Firshman²,

Stang

et al. 2012

BMC Vet Res

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BackgroundThe objective was to determine the effects of agility exercise on dogs of different skill levels with respect to urinary eicosanoids, urinary 15F2t-isoprostane (lipid peroxidation marker) and hematological/biochemical changes in plasma. Fifteen adult dogs had blood and urine samples obtained prior to, immediately and 4-hours following an agility exercise.ResultsHematocrit, red blood cells (RBC), albumin, and hemoglobin increased following exercise, with greatest increases correlating to increased skill group (novice, intermediate, masters); at 4-hours post-exercise, hematocrit, RBC, and hemoglobin were decreased. Phosphorus increased following exercise with the greatest increase in novice and intermediates. Plasma lactate increased 3.6-fold in masters, 3.2-fold in intermediates, and 1.2-fold in novice dogs. Urine thromboxane B2 (TXB2) more than tripled 4-hours post-exercise while 6-keto prostaglandin F1α (PGF1α, prostacyclin metabolite), prostaglandin E2 metabolites (13,14-dihydro-15-keto-prostaglandin A2 and 13,14-dihydro-15-keto-prostaglandin E2), and 13,14-dihydro-15-keto prostaglandin F2α were unaffected as determined by a competitive enzyme immunoassay and standardized by division with urine creatinine. Urine 15F2t-isoprostane increased insignificantly.ConclusionsAlterations in the plasma post-exercise were likely due to hemoconcentration from insensible water loss, splenic contraction and sympathetic stimulation while 4-hours later autohemodilution reduced RBC parameters. Elevations in plasma lactate and urinary TXB2 correlated with advanced skill level/speed of the dogs.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs

Baltzer

Firshman²,

Stang

et al. 2012

BMC Vet Res

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“…However, based on the findings of the current study, it would appear that there are also dramatic changes within the thoracic limbs. Most locomotor scoring systems used for assessment of spinal cord injury in dogs assess only the pelvic limbs . The gait score used in this study was adapted from a previous study on Doberman Pinschers with CSM with the goal of also taking into account the thoracic limbs.…”

Section: Discussionmentioning

confidence: 99%

Three‐Dimensional Kinematic Gait Analysis of Doberman Pinschers with and without Cervical Spondylomyelopathy

Foss

Costa

Moore

2012

Veterinary Internal Medicne

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Background The optimal treatment of cervical spondylomyelopathy (CSM) is controversial, with the owner’s and clinician’s perception of gait improvement often being used as outcome measures. These methods are subjective and suffer from observer bias. Objectives To establish kinematic gait parameters utilizing digital motion capture in normal Doberman Pinschers and compare them with CSM-affected Dobermans. Animals Nineteen Doberman Pinschers; 10 clinically normal and 9 with CSM. Methods All dogs were enrolled prospectively and fitted with a Lycra® body suit, and motion capture was performed and used to reconstruct a 3-D stick diagram representation of each dog based on 32 reflective markers, from which several parameters were measured. These included stride duration, length, and height; maximal and minimal spinal angles; elbow and stifle flexion and extension; and maximum and minimum distances between the thoracic and pelvic limbs. A random-effects linear regression model was used to compare parameters between groups. Results Significant differences between groups included smaller minimum (mean = 116 mm; P = .024) and maximum (mean = 184 mm; P = .001) distance between the thoracic limbs in CSM-affected dogs. Additionally, thoracic limb stride duration was also smaller (P = .009) in CSM-affected dogs (mean = 0.7 seconds) when compared with normal dogs (mean = 0.8 seconds). In the pelvic limbs, the average stifle flexion (mean = 100°; P = .048) and extension (mean = 136°; P = .009), as well as number of strides (mean = 2.7 strides; P = .033) were different between groups. Conclusions and Clinical Importance Our findings suggest that computerized gait analysis reveals more consistent kinematic differences in the thoracic limbs, which can be used as future objective outcome measures.

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“…A study of dogs with intervertebral disk disease (IVDD) evaluated the urine concentration of 8-isoprostane F2α before and after decompression surgery [18]. The concentrations of total isoprostane in dogs with similar focal SCIs were approximately three times higher than the focal SCIs in our study.…”

Section: Oxidative Stress Diagnostic Indicators For Myelomalaciamentioning

confidence: 94%

Increase in oxidative stress biomarkers in dogs with ascending–descending myelomalacia following spinal cord injury

Marquis¹,

Packer

Borgens³

et al. 2015

Journal of the Neurological Sciences

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Concentrations of 15F2t isoprostane in urine of dogs with intervertebral disk disease

Abstract: Urinary isoprostane-to-creatinine ratios were higher in dogs with IVDD before and after surgery. Analysis of these data suggests that dogs with IVDD are in a state of oxidative stress and that preemptive treatment with antioxidants warrants further investigation.

Cited by 25 publications

References 48 publications

The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs

The effect of agility exercise on eicosanoid excretion, oxidant status, and plasma lactate in dogs

Three‐Dimensional Kinematic Gait Analysis of Doberman Pinschers with and without Cervical Spondylomyelopathy

Increase in oxidative stress biomarkers in dogs with ascending–descending myelomalacia following spinal cord injury

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